Gene:
Gene Symbol:
MAP3K11
Gene Name:
mitogen-activated protein kinase kinase kinase 11
Original Clone ID:
FLH231723.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617941
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2544nts
Open reading frame: 494 to 3037
CGGACGCGTGGGGGAAGAAGGGAGCGGGGTCGGAGCCGTCGGGGCCAAAGGAGACGGGGC
CAGGAACAGGCAGTCTCGGCCCAACTGCGGACGCTCCCTCCACCCCCTGCGCAAAAAGAC
CCAACCGGAGTTGAGGCGCTGCCCCTGAAGGCCCCACCTTACACTTGGCGGGGGCCGGAG
CCAGGCTCCCAGGACTGCTCCAGAACCGAGGGAAGCTCGGGTCCCTCCAAGCTAGCCATG
GTGAGGCGCCGGAGGCCCCGGGGCCCCACCCCCCCGGCCTGACCACACTGCCCTGGGTGC
CCTCCTCCAGAAGCCCGAGATGCGGGGGGCCGGGAGACAACACTCCTGGCTCCCCAGAGA
GGCGTGGGTCTGGGGCTGAGGGCCAGGGCCCGGATGCCCAGGTTCCGGGACTAGGGCCTT
GGCAGCCAGCGGGGGTGGGGACCACGGGCACCCAGAGAAGGTCCTCCACACATCCCAGCG
CCGGCTCCCGGCCATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATG
GAATGGCAGTGGCAGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCC
AAAGGCAGCGGGTTATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGG
GCAGGATGAGCTGGCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGC
CATCTCAGGAGACGAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCC
GTCCAACTATGTGTCTCGGGGTGGTGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGA
GCTGCGGCTGGAGGAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTG
GCGAGGTGAGCTGGTGGCTGTGAAGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGT
GACAGCCGAGAGCGTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACAT
CATTGCCCTCAAGGCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGC
AGCCGGTGGGCCCCTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGT
CAACTGGGCTGTGCAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCCTGGTGCC
CGTCATCCACCGTGATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGA
CGACATGGAGCACAAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAA
AACCACACAAATGAGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGC
CTCCACCTTCTCTAAGGGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCT
GACCGGGGAGGTGCCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCGTAGCTGT
TAACAAGCTCACACTGCCCATCCCATCCACCTGCCCCGAGCCCTTCGCACAGCTTATGGC
CGACTGCTGGGCGCAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGCAGCAGTT
GGAGGCGCTGGAGGCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCA
GGAAGGCTGGAAGCGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGA
ACTACTGAGCCGCGAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGA
GCAGCTGCGGCGGCGCGAGCACCTGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGA
GCTGACGCTGCTGCTGCAGCAGGTGGACCGCGAGCGACCGCACGTGCGCCGCCGCCGCGG
GACATTCAAGCGCAGCAAGCTCCGGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACT
CGACTTCAAGCACCGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGT
CTTCGAGGTCGGGCCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGA
GCCTGCAGAGCCAGGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAG
CAATGGAGAGCGGCGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGC
CCAGAATGGGAGGAGAAGGTCCCGCATGGACGAAGCCACATGGTACCTGGATTCAGATGA
CTCATCCCCCTTAGGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCC
TAGCCTGGAGCCCGAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCAGCTCTGG
GACGCCCAAGCTGATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCTCGCTGGG
CCTTGGCCGCGACCTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGAC
AACACCCCCCACGCCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCAT
CTGCTTCTCGCTCAAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCT
GGGTATCCCTGTGGGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGG
AGGCACTGTCTCACCCCCACCGGGGACATCACGCTCTGCTCCTGGCACCCCAGGCACCCC
ACGTTCACCACCCCTGGGCCTCATCAGCCGACCTCGGCCCTCGCCCCTTCGCAGCCGCAT
TGATCCCTGGAGCTTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCC
TGCACCCCGCCGAGCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCC
ACCTGCCAACCCCTTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGG
TGCCCAGGCCCCGTGGGTGCCGGAAGCGGGGCCTTGAGTGGGCCAGGCCACTCCCCCGAG
CTCCAGCTGCCTTAGGAGGAGTCACAGCATACACTGGAACAGGAGCTGGGTCAGCCTCTG
CAGCTGCCTCAGTTTCCCCAGGGACCCCACCCCCCTTTGGGGGTCAGGAACACTACACTG
CACAGGAAGCCTTCACACTGGAAGGGGGACCTGCGCCCCCACATCTGAAACCTGTAGGTC
CCCCCAGCTCACCTGCCCTACTGGGGCCCAACACTGTACCCAGCTGGTTGGGAGGACCAG
AGCCTGTCTCAGGGAATTGCCTGCTGGGGTGATGCAGGGAGGAGGGGAGGTGCAGGGAAG
AGGGGCCGGCCTCAGCTGTCACCAGCACTTTTGACCAAGTCCTGCTACTGCGGCCCCTGC
CCTAGGGCTTAGAGCATGGACCTCCTGCCCTGGGGGTCATCTGGGGCCAGGGCTCTCTGG
ATGCCTTCCTGCTGCCCCAGCCAGGGTTGGAGTCTTAGCCTCGGGATCCAGTGAAGCCAG
AAGCCAAATAAACTCAAAAGCTGTCTCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA
Protein sequence predicted based on nucleotide sequence
MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDEL ALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLE EVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALK AVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHR DLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFS KGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWA QDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEGWKREIQGLFDELRAKEKELLSR EEELTRAAREQRSQAEQLRRREHLLAQWELEVFERELTLLLQQVDRERPHVRRRRGTFKR SKLRARDGGERISMPLDFKHRITVQASPGLDRRRNVFEVGPGDSPTFPRFRAIQLEPAEP GQAWGRQSPRRLEDSSNGERRACWAWGPSSPKPGEAQNGRRRSRMDEATWYLDSDDSSPL GSPSTPPALNGNPPRPSLEPEEPKRPVPAERGSSSGTPKLIQRALLRGTALLASLGLGRD LQPPGGPGRERGESPTTPPTPTPAPCPTEPPPSPLICFSLKTPDSPPTPAPLLLDLGIPV GQRSAKSPRREEEPRGGTVSPPPGTSRSAPGTPGTPRSPPLGLISRPRPSPLRSRIDPWS FVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFQGGPQDCRAQTKDMGAQAP WVPEAGP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
