DGKH (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  DGKH
Gene Name:  diacylglycerol kinase, eta
Original Clone ID:
FLH231557.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617981
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3303nts
Open reading frame: 90 to 3392

GGGGCCTTCAAGAAGAGAGTTCTCAGAGCAGACTCAGCTTTAGGTGCTGAAATGAATTTC
TCAAGATCATCACTCCATTCAGAAGGCTAATGCTGTGTGCTGAGAACAGAAAGGAGATGG
AGGATTGGATCAGCTCACTGAAGTCTGTACAGACCAGAGAACCCTACGAGGTGGCCCAGT
TTAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGACCCA
CCTTCTGTAACGTGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGCG
AAGTGTGTAAATTCAAGGCTCACAAAAGATGTGCAGTGAGAGCAACAAATAACTGTAAAT
GGACTACCCTGGCCTCCATCGGGAAGGACATTATAGAAGATGAAGATGGCGTCGCGATGC
CTCACCAGTGGCTTGAGGGCAACCTGCCTGTAAGTGCCAAGTGTGCTGTCTGCGACAAAA
CATGTGGCAGTGTTCTCCGTCTACAGGATTGGAAATGCCTTTGGTGTAAGACAATGGTAC
ACACTGCCTGCAAAGATTTATACCATCCAATATGTCCACTTGGTCAATGTAAAGTATCTA
TCATACCTCCAATTGCACTAAACAGCACCGATTCCGATGGTTTCTGTAGAGCAACATTTT
CGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATTCTAAGAGTGGAGATAATCAGGGAG
TAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGTGTTTGATTTAATGA
ATGGAGGTCCTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATTCTTG
TTTGTGGAGGCGATGGAAGTGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGA
ATAAACAGTGTCAGCTGGGAGTGTTGCCTTTGGGTACAGGAAATGACCTTGCACGAGTTC
TTGGCTGGGGAGGTTCATATGACGATGACACCCAACTTCCTCAGATCCTAGAGAAACTGG
AACGAGCCAGTACCAAAATGTTGGACAGGTGGAGTATAATGACATATGAACTCAAATTGC
CACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAGCATCTGAAGAATTTTATATGACGA
TTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTCTGATGAACATGCAG
TGGTCATATCTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAAGTAG
AAAAGACGTATGACAAAACCTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAAT
GTTCAGTCCTAAACGAGAAGCTCGAACAACTGCTGCAGGCTTTGCACACAGATTCCCAGG
CTGCGCCTGTTCTCCCTGGCCTCAGCCCTCTCATTGTGGAAGAAGATGCTGTGGAATCGT
CCAGTGAAGAGTCCCTGGGTGAAAGCAAAGAGCAGCTTGGGGATGACGTTACAAAACCTT
CCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGTTGCGGGCAAATAGTTTAAAGAAAG
CAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCCGACAGTTCACCCGT
GTGAACCAGCTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAGGAGG
AAGCTAAAGATGATGGTGCCAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAG
ATGCCCGGGCAAGTTATGGCCATTCCCAAACTGATTCTGTCCCTGGTCCAGCTGTGGCAG
CCAGCAAAGAAAACCTCCCTGTGCTCAATACCAGAATAATCTGCCCAGGTTTAAGAGCAG
GACTGGCTGCCTCAATTGCTGGGAGTTCGATTATCAACAAAATGTTACTGGCAAACATTG
ATCCTTTTGGTGCCACGCCGTTTATTGACCCGGATCTAGATTCCGTAGATGGATATTCAG
AAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGATGCAAAAATTTCATTAGAAT
TTAATAATAAAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAAAACTTGATGT
GGTATGGAGTCCTTGGAACCCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAAA
GGGTTCAACTTGAGTGTGATGGGCAGTATATTCCTCTTCCCAGCTTGCAAGGCATAGCCG
TGTTGAACATTCCCAGCTATGCTGGAGGCACTAACTTTTGGGGTGGAACTAAAGAGGATG
ATATATTTGCTGCACCATCCTTTGATGACAAGATCCTGGAAGTTGTAGCAATATTTGATA
GCATGCAAATGGCAGTTTCAAGGGTCATTAAACTGCAGCATCATCGAATAGCCCAGTGCC
GTACAGTGAAAATCACTATATTTGGTGACGAAGGAGTCCCAGTGCAAGTGGATGGTGAAG
CGTGGGTTCAGCCTCCAGGGATTATCAAAATTGTGCACAAAAACAGAGCACAAATGCTAA
CAAGGGACAGAGCCTTTGAGAGCACTCTGAAATCTTGGGAAGATAAGCAGAAGTGTGATT
CTGGTAAACCAGTTCTCCGAACCCATTTGTACATCCATCACGCCATTGACTTGGCAACAG
AAGAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTACTAGGATAT
GTGACGCAGCCACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGCTGTGAATGCCT
GCTCCCATGCCCTGAATAAAGCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACTG
CCACTGAAATAGCCATCAATGTGAAGGCGCTGTATAATGAAACAGAATCTTTGCTAGTTG
GCAGGGTTCCTTTGCAGCTGGAATCGCCACATGAAGAGCGAGTATCCAATGCCTTACACT
CTGTGGAGGTGGAATTACAGAAACTGACAGAGATTCCTTGGCTTTATTATATCTTACACC
CAAATGAGGATGAGGAACCTCCTATGGATTGCACCAAAAGGAACAACAGAAGCACCGTAT
TTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGAAGCAGAAGACAAGTTCACAGC
CTGTTCAGAAATGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCTCAATTTGGGAG
AGTACAAAGATATCTTCATCCGTCATGACATCAGAGGGGCTGAACTTTTGCATCTGGAAA
GGCGAGATCTTAAGAACACAGTAGGAGAAAAAAGAGACACCAAAGAAAATGGTAAACATA
TGGATCTGGGGATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAATTAAAGAGC
TTGGAAGGAGCACTCCACAGTCGGAGGTGTAATCATATTGGTGCTATTTCTTGGAAGAGA
AGTTATTGCCACTTAATACAAAGTCCTTGGAAGCAAGTGGCTGTTCTTGTAGTTTTCTGC
ATAGATAAGTAAGCACCACTGAAGCACCTCTGTGGCTTGATATTTTGCTGTGGGTGAAAT
TTTGATTTGAGGTATTAGAAAATATTTTTGTGCCGAACAATACATTCCACAAAGCCATTT
TCTTTTTGTGCAAACCTGACATGTTCAAATATATTCACAATGGTAATAAGGTAGGAGGAA
TCTGAGACGATTGCATTGTCTAAACAGTGGAATTGCAAAATGTTTACTTTCCATAGATCT
GGTCTGATGGAATGTTCCTACTGTGCAACTGCATAACAATATGTGGTTAAAACTTCCTAG
GTTTCATTACGGGGGGGCATTGGCCATTTTCTTAAAATCATAGGTTAGGAGACTAAAATA
TAAATTAAGTTGTGATTTGAATGTAGATTGGTATCACCTCCAACTCCTAGTGCTTAGTGG
TCAAATATGTTGAAAACCTTTCCAATACTATGAATGAATGCAAATCTTAATGCACGAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESL
SGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLP
VSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNST
DSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRL
FQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDD
TQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHL
TKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQ
LLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPRE
IMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKES
ITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSS
IINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPE
KCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGG
TNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGD
EGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHL
YIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANP
RCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLT
EIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEV
AAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKNTVGEKRDTKENGKHMDLGIPKVGH
VKRILQGIKELGRSTPQSEV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable