Gene:
Gene Symbol:
ERBB4
Gene Name:
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
Original Clone ID:
FLH231814.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617986
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
AATTGTCAGCACGGGATCTGAGACTTCCAAAAAATGAAGCCGGCGACAGGACTTTGGGTC
TGGGTGAGCCTTCTCGTGGCGGCGGGGACCGTCCAGCCCAGCGATTCTCAGTCAGTGTGT
GCAGGAACGGAGAATAAACTGAGCTCTCTCTCTGACCTGGAACAGCAGTACCGAGCCTTG
CGCAAGTACTATGAAAACTGTGAGGTTGTCATGGGCAACCTGGAGATAACCAGCATTGAG
CACAACCGGGACCTCTCCTTCCTGCGGTCTGTTCGAGAAGTCACAGGCTACGTGTTAGTG
GCTCTTAATCAGTTTCGTTACCTGCCTCTGGAGAATTTACGCATTATTCGTGGGACAAAA
CTTTATGAGGATCGATATGCCTTGGCAATATTTTTAAACTACAGAAAAGATGGAAACTTT
GGACTTCAAGAACTTGGATTAAAGAACTTGACAGAAATCCTAAATGGTGGAGTCTATGTA
GACCAGAACAAATTCCTTTGTTATGCAGACACCATTCATTGGCAAGATATTGTTCGGAAC
CCATGGCCTTCCAACTTGACTCTTGTGTCAACAAATGGTAGTTCAGGATGTGGACGTTGC
CATAAGTCCTGTACTGGCCGTTGCTGGGGACCCACAGAAAATCATTGCCAGACTTTGACA
AGGACGGTGTGTGCAGAACAATGTGACGGCAGATGCTACGGACCTTACGTCAGTGACTGC
TGCCATCGAGAATGTGCTGGAGGCTGCTCAGGACCTAAGGACACAGACTGCTTTGCCTGC
ATGAATTTCAATGACAGTGGAGCATGTGTTACTCAGTGTCCCCAAACCTTTGTCTACAAT
CCAACCACCTTTCAACTGGAGCACAATTTCAATGCAAAGTACACATATGGAGCATTCTGT
GTCAAGAAATGTCCACATAACTTTGTGGTAGATTCCAGTTCTTGTGTGCGTGCCTGCCCT
AGTTCCAAGATGGAAGTAGAAGAAAATGGGATTAAAATGTGTAAACCTTGCACTGACATT
TGCCCAAAAGCTTGTGATGGCATTGGCACAGGATCATTGATGTCAGCTCAGACTGTGGAT
TCCAGTAACATTGACAAATTCATAAACTGTACCAAGATCAATGGGAATTTGATCTTTCTA
GTCACTGGTATTCATGGGGACCCTTACAATGCAATTGAAGCCATAGACCCAGAGAAACTG
AACGTCTTTCGGACAGTCAGAGAGATAACAGGTTTCCTGAACATACAGTCATGGCCACCA
AACATGACTGACTTCAGTGTTTTTTCTAACCTGGTGACCATTGGTGGAAGAGTACTCTAT
AGTGGCCTGTCCTTGCTTATCCTCAAGCAACAGGGCATCACCTCTCTACAGTTCCAGTCC
CTGAAGGAAATCAGCGCAGGAAACATCTATATTACTGACAACAGCAACCTGTGTTATTAT
CATACCATTAACTGGACAACACTCTTCAGCACAATCAACCAGAGAATAGTAATCCGGGAC
AACAGAAAAGCTGAAAATTGTACTGCTGAAGGAATGGTGTGCAACCATCTGTGTTCCAGT
GATGGCTGTTGGGGACCTGGGCCAGACCAATGTCTGTCGTGTCGCCGCTTCAGTAGAGGA
AGGATCTGCATAGAGTCTTGTAACCTCTATGATGGTGAATTTCGGGAGTTTGAGAATGGC
TCCATCTGTGTGGAGTGTGACCCCCAGTGTGAGAAGATGGAAGATGGCCTCCTCACATGC
CATGGACCGGGTCCTGACAACTGTACAAAGTGCTCTCATTTTAAAGATGGCCCAAACTGT
GTGGAAAAATGTCCAGATGGCTTACAGGGGGCAAACAGTTTCATTTTCAAGTATGCTGAT
CCAGATCGGGAGTGCCACCCATGCCATCCAAACTGCACCCAAGGGTGTAACGGTCCCACT
AGTCATGACTGCATTTACTACCCATGGACGGGCCATTCCACTTTACCACAACATGCTAGA
ACTCCCCTGATTGCAGCTGGAGTAATTGGTGGGCTCTTCATTCTGGTCATTGTGGGTCTG
ACATTTGCTGTTTATGTTAGAAGGAAGAGCATCAAAAAGAAAAGAGCCTTGAGAAGATTC
TTGGAAACAGAGTTGGTGGAACCATTAACTCCCAGTGGCACAGCACCCAATCAAGCTCAA
CTTCGTATTTTGAAAGAAACTGAGCTGAAGAGGGTAAAAGTCCTTGGCTCAGGTGCTTTT
GGAACGGTTTATAAAGGTATTTGGGTACCTGAAGGAGAAACTGTGAAGATTCCTGTGGCT
ATTAAGATTCTTAATGAGACAACTGGTCCCAAGGCAAATGTGGAGTTCATGGATGAAGCT
CTGATCATGGCAAGTATGGATCATCCACACCTAGTCCGGTTGCTGGGTGTGTGTCTGAGC
CCAACCATCCAGCTGGTTACTCAACTTATGCCCCATGGCTGCCTGTTGGAGTATGTCCAC
GAGCACAAGGATAACATTGGATCACAACTGCTGCTTAACTGGTGTGTCCAGATAGCTAAG
GGAATGATGTACCTGGAAGAAAGACGACTCGTTCATCGGGATTTGGCAGCCCGTAATGTC
TTAGTGAAATCTCCAAACCATGTGAAAATCACAGATTTTGGGCTAGCCAGACTCTTGGAA
GGAGATGAAAAAGAGTACAATGCTGATGGAGGAAAGATGCCAATTAAATGGATGGCTCTG
GAGTGTATACATTACAGGAAATTCACCCATCAGAGTGACGTTTGGAGCTATGGAGTTACT
ATATGGGAACTGATGACCTTTGGAGGAAAACCCTATGATGGAATTCCAACGCGAGAAATC
CCTGATTTATTAGAGAAAGGAGAACGTTTGCCTCAGCCTCCCATCTGCACTATTGACGTT
TACATGGTCATGGTCAAATGTTGGATGATTGATGCTGACAGTAGACCTAAATTTAAGGAA
CTGGCTGCTGAGTTTTCAAGGATGGCTCGAGACCCTCAAAGATACCTAGTTATTCAGGGT
GATGATCGTATGAAGCTTCCCAGTCCAAATGACAGCAAGTTCTTTCAGAATCTCTTGGAT
GAAGAGGATTTGGAAGATATGATGGATGCTGAGGAGTACTTGGTCCCTCAGGCTTTCAAC
ATCCCACCTCCCATCTATACTTCCAGAGCAAGAATTGACTCGAATAGGAGTGAAATTGGA
CACAGCCCTCCTCCTGCCTACACCCCCATGTCAGGAAACCAGTTTGTATACCGAGATGGA
GGTTTTGCTGCTGAACAAGGAGTGTCTGTGCCCTACAGAGCCCCAACTAGCACAATTCCA
GAAGCTCCTGTGGCACAGGGTGCTACTGCTGAGATTTTTGATGACTCCTGCTGTAATGGC
ACCCTACGCAAGCCAGTGGCACCCCATGTCCAAGAGGACAGTAGCACCCAGAGGTACAGT
GCTGACCCCACCGTGTTTGCCCCAGAACGGAGCCCACGAGGAGAGCTGGATGAGGAAGGT
TACATGACTCCTATGCGAGACAAACCCAAACAAGAATACCTGAATCCAGTGGAGGAGAAC
CCTTTTGTTTCTCGGAGAAAAAATGGAGACCTTCAAGCATTGGATAATCCCGAATATCAC
AATGCATCCAATGGTCCACCCAAGGCCGAGGATGAGTATGTGAATGAGCCACTGTACCTC
AACACCTTTGCCAACACCTTGGGAAAAGCTGAGTACCTGAAGAACAACATACTGTCAATG
CCAGAGAAGGCCAAGAAAGCGTTTGACAACCCTGACTACTGGAACCACAGCCTGCCACCT
CGGAGCACCCTTCAGCACCCAGACTACCTGCAGGAGTACAGCACAAAATATTTTTATAAA
CAGAATGGGCGGATCCGGCCTATTGTGGCAGAGAATCCTGAATACCTCTCTGAGTTCTCC
CTGAAGCCAGGCACTGTGCTGCCGCCTCCACCTTACAGACACCGGAATACTGTGGTGTAA
GCTCAGTTGTGGTTTTTTAGGTGGAGAGACACACCTGCTCCAATTTCCCCACCCCCCTCT
CTTTCTCTGGTGGTCTTCCTTCTACCCCAAGGCCAGTAGTTTTGACACTTCCCAGTGGAA
GATACAGAGATGCAATGATAGTTATGTGCTTACCTAACTTGAACATTAGAGGGAAAGACT
GAAAGAGAAAGATAGGAGGAACCACAATGTTTCTTCATTTCTCTGCATGGGTTGGTCAGG
AGAATGAAACAGCTAGAGAAGGACCAGAAAATGTAAGGCAATGCTGCCTACTATCAAACT
AGCTGTCACTTTTTTTCTTTTTCTTTTTCTTTCTTTGTTTCTTTCTTCCTCTTCTTTTTT
TTTTTTTTTTTAAAGCAGATGGTTGAAACACCCATGCTATCTGTTCCTATCTGCAGGAAC
TGATGTGTGCATATTTAGCATCCCTGGAAATCATAATAAAGTTTCCATTAGAACAAAAGA
ATAACATTTTCTATAACATATGATAGTGTCTGAAATTGAGAATCCAGTTTCTTTCCCCAG
CAGTTTCTGTCCTAGCAAGTAAGAATGGCCAACTCAACTTTCATAATTTAAAAATCTCCA
TTAAAGTTATAACTAGTAATTATGTTTTCAACACTTTTTGGTTTTTTTCATTTTGTTTTG
CTCTGACCGATTCCTTTATATTTGCTCCCCTATTTTTGGCTTTAATTTCTAATTGCAAAG
ATGTTTACATCAAAGCTTCTTCACAGAATTTAAGCAAGAAATATTTTAATATAGTGAAAT
GGCCACTACTTTAAGTATACAATCTTTAAAATAAGAAAGGGAGGCTAATATTTTTCATGC
TATCAAATTATCTTCACCCTCATCCTTTACATTTTTCAACATTTTTTTTTCTCCATAAAT
GACACTACTTGATAGGCCGTTGGTTGTCTGAAGAGTAGAAGGGAAACTAAGAGACAGTTC
TCTGTGGTTCAGGAAAACTACTGATACTTTCAGGGGTGGCCCAATGAGGGAATCCATTGA
ACTGGAAGAAACACACTGGATTGGGTATGTCTACCTGGCAGATACTCAGAAATGTAGTTT
GCACTTAAGCTGTAATTTTATTTGTTCTTTTTCTGAACTCCATTTTGGATTTTGAATCAA
GCAATATGGAAGCAACCAGCAAATTAACTAATTTAAGTACATTTTTAAAAAAAGAGCTAA
GATAAAGACTGTGGAAATGCCAAACCAAGCAAATTAGGAACCTTGCAACGGTATCCAGGG
ACTATGATGAGAGGCCAGCACATTATCTTCATATGTCACCTTTGCTACGCAAGGAAATTT
GTTCAGTTCGTATACTTCGTAAGAAGGAATGCGAGTAAGGATTGGCTTGAATTCCATGGA
ATTTCTAGTATGAGACTATTTATATGAAGTAGAAGGTAACTCTTTGCACATAAATTGGTA
TAATAAAAAGAAAAACACAAACATTCAAAGCTTAGGGATAGGTCCTTGGGTCAAAAGTTG
TAAATAAATGTGAAACATCTTCTC
Protein sequence predicted based on nucleotide sequence
MKPATGLWVWVSLLVAAGTVQPSDSQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVM GNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIF LNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVST NGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSG PKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVD SSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCT KINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNL VTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFST INQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYD GEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGA NSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHARTPLIAAGVIGG LFILVIVGLTFAVYVRRKSIKKKRALRRFLETELVEPLTPSGTAPNQAQLRILKETELKR VKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEALIMASMDHPHL VRLLGVCLSPTIQLVTQLMPHGCLLEYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLV HRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQ SDVWSYGVTIWELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYMVMVKCWMID ADSRPKFKELAAEFSRMARDPQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAE EYLVPQAFNIPPPIYTSRARIDSNRSEIGHSPPPAYTPMSGNQFVYRDGGFAAEQGVSVP YRAPTSTIPEAPVAQGATAEIFDDSCCNGTLRKPVAPHVQEDSSTQRYSADPTVFAPERS PRGELDEEGYMTPMRDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHNASNGPPKAED EYVNEPLYLNTFANTLGKAEYLKNNILSMPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQ EYSTKYFYKQNGRIRPIVAENPEYLSEFSLKPGTVLPPPPYRHRNTVV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
