SPHK1 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SPHK1
Gene Name:  sphingosine kinase 1
Original Clone ID:
FLH232507.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617988
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1155nts
Open reading frame: 339 to 1493

GGCACGAGGGAGAGGCCGGGGAGTCCGCTCCAGCGGGGCGCTCCAGTCCCTCAGACGTGG
GCTGAGCTTGGGACGAGCTGCGTTCCGCCCCAGGCCACTGTAGGGAACGGCGGTGGCGCC
TCCCCAGCAAACCGGACCGACTGGGTCCAGCCGCCGCAGGGAATGACGCCGGTGCTCCTA
CAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCGGCCCTGCGACGCCCGCCTGG
GCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCCCCCACAGCGC
CAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCCCC
GGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGG
GCAAGGCCTTGCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCT
CCTTCACGCTGATGCTCACTGAGCGGCGGAACCACGCGCGGGAGCTGGTGCGGTCGGAGG
AGCTGGGCCGCTGGGACGCTCTGGTGGTCATGTCTGGAGACGGGCTGATGCACGAGGTGG
TGAACGGGCTCATGGAGCGGCCTGACTGGGAGACCGCCATCCAGAAGCCCCTGTGTAGCC
TCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCTGGCTATGAGC
AGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGT
CACCCATGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGCCTCTTCTCTGTGCTCA
GCCTGGCCTGGGGCTTCATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGG
GGGAGATGCGCTTCACTCTGGGCACCTTCCTGCTTCTGGCAGCCCTGCGCACCTACCGCG
GCCGACTGGCCTACCTCCCTGTAGGAAGAGTGGGTTCCAAGACACCTGCCTCCCCCGTTG
TGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACTGGAGGAGCCAGTGCCCTCTC
ACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCACTGCTGCACTCGCACC
TGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGT
TCTACGTGCGGGCGGGAGTGTCTCGTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGA
AGGGCAGGCATATGGAGTATGAATGCCCCTACTTGGTATACGTGCCCGTGGTCGCCTTCC
GCTTGGAGCCCAAGGATGGGAAAGGTGTGTTTGCAGTGGATGGGGAATTGATGGTTAGCG
AGGCCGTGCAGGGCCAGGTGCACCCAAACTACTTCTGGATGGTCAGCGGTTGCGTGGAGC
CCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGAAGAGCCCTTATGACCCCTGG
GCCGCGCTGTGCCTTAGTGTCTACTTGCAGGACCCTTCCTCCTTCCCTAGGGCTGCAGGG
CCTGTCCACAGCTCCTGTGGGGGTGGAGGAGACTCCTCTGGAGAAGGGTGAGAAGGTGGA
GGCTATGCTTTGGGGGGACAGGCCAGAATGAAGTCCTGGGTCAGGAGCCCAGCTGGCTGG
GCCCAGCTGCCTATGTAAGGCCTTCTAGTTTGTTCTGAGACCCCCCCCCCACGAACCAAA
TCCAAATAAAGTGACATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHA
RELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASL
NHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLE
SEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVP
LEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLL
RLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFW
MVSGCVEPPPSWKPQQMPPPEEPL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable