Gene:
Gene Symbol:
PIP5K1A
Gene Name:
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Original Clone ID:
FLH231903.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617991
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1503nts
Open reading frame: 454 to 1956
GGCACGAGGATTTTTTGCTTGGCTACCCGGAGTGAAGCGGCCGGGTTGGGCGATTAACAG
GCCGTGGTTAGGAAGGACGGAGAAGGGGCGTTCGCTCCTTTGGGACTTTTCATGCCTCGT
TTTTTTTTCAGATGTGGCTTGGTCTGGGCGCAAGGTCCCAGCAGCCAGCTTAAGCTTACT
CTTCTGTGAAAGGGGAAAGTATCCCCTGTGGAAAGCGGTTAAACTTGTGGAGGGGGTGCG
GGACGTGAGTTCTTCCCCATGCCAGGCGAATGGTGTGGCCTTGAGCTGGTCCAGGAGCCG
GCTCGACGTGTCTGAGGGAGGCCCCGGAGGGGGCGGGGAGGTGGCCCACAGAACGCGGGT
TCTGTAAAGAGACGTTGGGAAGATTCGATTCCGAGAAGAGGAAGAACCGGATTGAAAGAG
AGCCAGGCCGCTGAGGGGGAGGGGGCTGCTAAGATGGCGTCGGCCTCCTCCGGGCCGTCG
TCTTCGGTCGGTTTTTCATCCTTTGATCCCGCGGTCCCTTCCTGTACCTTGTCCTCAGCA
TCTGGAATCAAGAGACCCATGGCATCTGAGGTGCCTTATGCCTCTGGCATGCCCATCAAG
AAAATAGGCCATAGAAGTGTTGATTCCTCAGGAGAGACAACATATAAAAAGACAACCTCA
TCAGCCTTGAAAGGTGCCATCCAGTTAGGCATTACCCACACTGTGGGGAGCCTGAGTACC
AAACCAGAGCGTGATGTCCTCATGCAAGATTTCTACGTGGTTGAGAGTATCTTCTTTCCC
AGTGAAGGGAGCAACCTGACCCCTGCTCATCACTACAATGACTTTCGTTTCAAGACCTAT
GCACCTGTTGCCTTCCGCTACTTCCGGGAGCTATTTGGTATCCGGCCCGATGATTACTTG
TATTCCCTCTGCAGTGAGCCGCTGATTGAACTCTGTAGCTCTGGAGCTAGTGGTTCCCTA
TTCTATGTGTCCAGCGACGATGAGTTCATTATTAAGACAGTCCAACATAAAGAGGCGGAA
TTTCTGCAGAAGCTGCTTCCAGGATACTACATGAACCTCAACCAGAACCCTCGGACTTTG
CTGCCTAAATTCTATGGACTGTACTGTGTGCAGGCAGGTGGCAAGAACATTCGGATTGTG
GTGATGAACAATCTTTTACCAAGATCGGTAAAAATGCATATCAAATATGACCTCAAAGGC
TCAACCTACAAACGGCGGGCTTCCCAGAAAGAGCGAGAGAAGCCTCTTCCCACATTTAAA
GACCTAGACTTCTTACAAGACATCCCTGATGGTCTTTTTTTGGATGCTGACATGTACAAC
GCTCTCTGTAAGACCCTGCAGCGTGACTGTTTGGTGCTGCAGAGCTTCAAGATAATGGAT
TATAGCCTCTTGATGTCAATCCATAATATAGATCATGCACAACGAGAGCCCTTAAGCAGT
GAAACACAGTACTCAGTTGATACTCGAAGACCGGCCCCCCAAAAGGCTCTGTATTCCACA
GCCATGGAATCCATCCAGGGAGAGGCTCGACGGGGTGGTACCATGGAGACTGATGACCAT
ATGGGTGGCATCCCTGCCCGGAATAGTAAAGGGGAAAGGCTTCTGCTTTATATTGGCATC
ATTGACATTCTACAGTCTTACAGGTTTGTTAAGAAGTTGGAGCACTCTTGGAAAGCCCTG
GTACATGACGGAGACACTGTCTCAGTGCATCGCCCAGGCTTCTACGCTGAACGGTTCCAG
CGCTTCATGTGCAACACAGTATTTAAGAAGATTCCCTGCGTTCACCTTGGTCGTCCTGAT
GTTTTACCTCAGACTCCACCTTTGGAGGAAATCAGTGAGGGCTCGCCTATTCCTGACCCC
AGTTTCTCACCTCTAGTTGGAGAGACTTTGCAAATGCTAACTACAAGTACAACCTTGGAA
AAGCTTGAAGTTGCAGAGTCAGAGTTCACCCATTAAGCGCAAAGCCTCAGAAGACCTGGA
ACAAGATTCTGCCATCTCTGTGATCCCAAGATGTCAGCCCTTGCCCCAGCAATGCTGAAT
TTTCTTCTACTTGGTCATCAAAAAAGGAGTGTAATAGAAGTGAGGGGAGCTGCTCCTCCA
TCTTCTTCCTGAAGAAGAACCTTCTCTCCTTCCTCTTCCTCATGAATGGGCCTTAGTGCC
TCAGAGAGTTGAGGACCGCAGCATCCCCTCCACTCCAGAGTTGGGTGGTACGGATTTTCA
ACTGGCCAACCCTTTGCCTCCACTATTGAATTTTTTTCAGACCCCCATTCTTCATGCTGG
AAATGGGATTGCTGGACTTGGCAGCTTTCTTTCCCCTCGTCTTTGACTAGGAACCGGACT
CTTAATTTCCTCAGGACAGACTAGCTGGCACATTATCCCTACCTTAGTTCTTTCTCTCTG
ACTCCTGGAAGAATACTCCTGTAATCTCTGTAAAGGTTTTTGGGGGATAAGGGTGTTTAA
CCACCTCCCAGCTTTCTTCTTCTTTTTTTTTTTCTGAAAAAAGGAAAACACACAGCACAC
AATTTCAAGCCATTTTCAGATCAGAACTCCAGAAGTGTTGACAAGATGCCTATTCGTAGA
GTTCCCTCAGAAGAGCCATGGTGTTTATGAAGAGAAGAGTAGTGATTGCTCTGCCAGAAG
CAGCTCCTCTTTAAACTCCTCCTCTCTTGATGAATTTCTTAAGGCTGAAGGAATGAAGAG
AGTGGGACATGGGGTAATCTTTATCCCTTTTGTTAAAACAGGAGGCAGCCATGGGCTGGG
AGATCATAGCCCTTCCTAGGCAGAATCCTGTTCACTGCCAGGCTATAGTAATTATTACTA
TTTTGCAATTTGAAATATATTCTGGTTGTTTTTCTAAATGTGAAGACTTACCAAATGAAT
TTTAGATCATTCTCCAGAGGAGATTTTTTTTGCTCTTCTCATCTTTTCCAACAGTGTTCT
CCTGTTTGTGGAGCTAAGGTAAAGAGGGGACACTTCTGTCTGTTTAACAGACAGTCCATA
TCTGTGAGGCCAGCAAATATTTTCTTAAACTCATGGGGAGACAGCAGATTCTTGCCTTGG
TGAGGTCATTGCTGTGCCATATGTCCTACCCCCCTGTCTTCATGCAGGGAAGTTGGAAAT
GGGGGCTACATATGCCCTCTCCTCCCCGTCTACAAGAGTTGTGGTTTTCCATCTGATCCT
TCCACTCTTGTCAGGGGAAGAAGGGGGCCTGGTATCTCAGGCAGATTGTTGAATTCCTGT
TCTATCCCTTCTCTATCCCACCCTGCCTTGATAATATGTTAGCCCATACCCCAAATAACT
GTCTATATTAGACACCCCCAGCCAGTTTCTGGCTGCCTGTCTTTGCTGCCATGTTTTTTA
CAAGAAGGAAAGAATTCTTGCTATTTTTTTTTCATAATTTACTATTTATGATGTATTTAA
GTGTTTTATTAAGGACAGAGTTCTGTTAGGGGTGGGAGGGAATATTTGAGGGAGGGCTGG
GTCTTAGGGAAAGGAATGGGGAAGCAACATTTTTATTAAGTGTTACTATTTGCCTCTACT
TTGTATTGTTCAGAAATGGCAAATACAATATAAAAGTGATATATGGTTTTAATGTAATAA
ACTTTAATGAGTTATTTAGAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MASASSGPSSSVGFSSFDPAVPSCTLSSASGIKRPMASEVPYASGMPIKKIGHRSVDSSG ETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHH YNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYVSSDDEFII KTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVK MHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCL VLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARR GGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHR PGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQ MLTTSTTLEKLEVAESEFTH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
