PRKG1 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PRKG1
Gene Name:  protein kinase, cGMP-dependent, type I
Original Clone ID:
HsCD00616911
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617999
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2061nts
Open reading frame: 59 to 2119

AGGAAGCCTCAAGACGCGGAGCAGCGGCAGGAAGGAGCCCCCGGCAGCCCGGAGGAGCAT
GGGCACCTTGCGGGATTTACAGTACGCGCTCCAGGAGAAGATCGAGGAGCTGAGGCAGCG
GGATGCTCTCATCGACGAGCTGGAGCTGGAGTTGGATCAGAAGGACGAACTGATCCAGAA
GCTGCAGAACGAGCTGGACAAGTACCGCTCGGTGATCCGACCAGCCACCCAGCAGGCGCA
GAAGCAGAGCGCGAGCACCTTGCAGGGCGAGCCGCGCACCAAGCGGCAGGCGATCTCCGC
CGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCATGTGACCCTGCCCTTCTACCCCAA
GAGCCCACAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGACTTTATGAAGAA
CTTGGAGCTGTCGCAGATCCAGGAGATTGTGGATTGTATGTACCCGGTGGAGTATGGCAA
GGACAGTTGCATCATCAAAGAAGGAGACGTGGGGTCACTGGTGTATGTCATGGAAGATGG
TAAGGTTGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCCAGGAAAAGTGTT
TGGGGAATTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAA
TGTAAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATAATGATGAGGACAGGACT
CATCAAGCATACCGAGTATATGGAATTTTTAAAAAGCGTTCCAACATTCCAGAGCCTTCC
TGAAGAGATCCTCAGCAAGCTTGCTGATGTCCTTGAAGAGACCCACTATGAAAATGGAGA
ATATATTATCAGGCAAGGTGCAAGAGGGGACACCTTCTTTATCATCAGCAAAGGAACGGT
AAATGTCACTCGTGAAGACTCACCGAGTGAAGACCCAGTCTTTCTTAGAACTTTAGGAAA
AGGAGACTGGTTTGGAGAGAAAGCCTTGCAGGGGGAAGATGTGAGAACAGCAAACGTAAT
TGCTGCAGAAGCTGTAACCTGCCTTGTGATTGACAGAGACTCTTTTAAACATTTGATTGG
AGGGCTGGATGATGTTTCTAATAAAGCATATGAAGATGCAGAAGCTAAAGCAAAATATGA
AGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCTGTCTGATTTCAACATCATTGATACCCT
TGGAGTTGGAGGTTTCGGACGAGTAGAACTGGTCCAGTTGAAAAGTGAAGAATCCAAAAC
GTTTGCAATGAAGATTCTCAAGAAACGTCACATTGTGGACACAAGACAGCAGGAGCACAT
CCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTCATAGTGAGACTGTACAG
AACATTTAAGGACAGCAAATATTTGTATATGTTGATGGAAGCTTGTCTAGGTGGAGAGCT
CTGGACCATTCTCAGGGATAGAGGTTCGTTTGAAGATTCTACAACCAGATTTTACACAGC
ATGTGTGGTAGAAGCTTTTGCCTATCTGCATTCCAAAGGAATCATTTACAGGGACCTCAA
GCCAGAAAATCTCATCCTAGATCACCGAGGTTATGCCAAACTGGTTGATTTTGGCTTTGC
AAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCCAGAGTATGTAGC
CCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCCGACTACTGGTCACTGGGAAT
CCTAATGTATGAACTCCTGACTGGCAGCCCACCTTTCTCAGGCCCAGATCCTATGAAAAC
CTATAACATCATATTGAGGGGGATTGACATGATAGAATTTCCAAAGAAGATTGCCAAAAA
TGCTGCTAATTTAATTAAAAAACTATGCAGGGACAATCCATCAGAAAGATTAGGGAATTT
GAAAAATGGAGTAAAAGACATTCAAAAGCACAAATGGTTTGAGGGCTTTAACTGGGAAGG
CTTAAGAAAAGGTACCTTGACACCTCCTATAATACCAAGTGTTGCATCACCCACAGACAC
AAGTAATTTTGACAGTTTCCCTGAGGACAACGATGAACCACCACCTGATGACAACTCAGG
ATGGGATATAGACTTCTAATGTATTTCTCTTACCTGCTTCTGCCTTGCTGAAGACAGCTT
TTTCTGAGACACAGCTGCCAGCAAACCTGAGGGAAAGAGAGAAGATTAGTGCTCGGGGTC
ACCATGATGCCTTTGATCGATGCTGCTCCAGTAACTACAGTGGCATTAGGACTTATCGCT
TAGATGACAATAGTGCTCTTTACATGTTTTCTGTTTGAACCTAAAATAGCAGTTGACATG
GTGGTCCTGAAGCAAAGCCTTTCACCAGTAAAGAGATGTTTTCTATTGTTGCAATGACCT
TGCTTTGCTCTGATTATAATTTGAAAGACTGTAGGAAACACTTCAATGTAGTATAAGAGT
CTGTACCTTGCTGGAATATTCAAGAAGATGAAAGAATAATATATTGGGTACAATAGATTA
CTATGGTACAGAAACTGGGCTATTCCCTTTCTTCAAGTGAAGGCTGTGGGATCTATTACA
GCTGCAGGCCGGTGTATATACCATACAAAAGAGGACCACACATCTGTTGGTCACAGAGTT
CATGTCACACCAGTGCTAGAAGTTTCATGATTTTATTTCCCAGCAGTGCTGATGACAAGA
CTGAATGTTACCTTTTCTTTCTGACAGATTTTAAAAATTGATATGATAAAAGCACAACTG
CTATAGATTCTGCTGAGACCTCTCATAGTAGGTATATATGAGTTTTCACAGAAGACTGAA
AAATAATGCATGATATTTGTTTGTTTTTTTTGATAAATTGGCATGACAGAGTGGGGAAAA
AAAGCAATTCACAAAACCATTTCATATTTTTTAAAATATTGTGCTTAAAGATGGTCCTGG
AAGTAAATGACTAGCAGCCAATTGGTTTTACTTAACATACCCTCAAACTGAGGCTTAAAG
TATTCCCTTTTATAAAAATAAATGCTTGGGGTAGGGTGGAGTGGGGAGGGATTAAAACCC
ATCCAAAAAATAAATAAAAACTATATAGGTGCTATGTATATCTTTCATCTGTAAATGTCA
GTGTCTGAACAGCAACACAAATTCAAATCATTATACGTGTAGCCAGAAACTCAAGCATTT
TCACTAAAGTTATTAAACCAAACTCCTGTCCAATTTGACTTATACAACATAGTCAGTCTA
GAGTTGAGAGACAAAGGTAATTATAAACCTATTTGAACTAGCTTCTTGTCTTAGGCCTGA
ACCAAAAAACAACAAACAAACAAAAAACAAGAATGAAAAACAGAAATAAAAGAAGTAGAA
AAGACAAAGAAAGAAAGCCCAAAGTCAAAGTTGTTAATATTTACAGGTTTACCAGATCTG
GAACATTACTTATTTGAGGTCAGAGAACAAAACAAGAACCTGGCCAGGTGTTGATTACCT
TTTAGTGAATAAGCTGAGTCCATATACTTGTCTAACTAAGAAAGCAGTACAGAGGAAAAC
AGGAACCTGATTTTTTTAAAATAAATTTTAAATAAAATAGAATTACTACAATTCTGCAAT
TTCATACTACCTAAAAAAGACTAGATTTGAAAATGTCAAGCTGATTTACTTTATTCACAT
GGAGAAAAGAATCCACAAATTAAACTGAGTCCTTCACTGGCATGCCAGTTGACTATTATT
AGCTGTCATAAGTAACCCCG

Protein sequence predicted based on nucleotide sequence

MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQA
QKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMK
NLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKV
FGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSL
PEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLG
KGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKY
EAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEH
IRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYT
ACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYV
APEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAK
NAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTD
TSNFDSFPEDNDEPPPDDNSGWDIDF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable