Gene:
Gene Symbol:
PKN2
Gene Name:
protein kinase N2
Original Clone ID:
HsCD00298527
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618001
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GGAGCGCAAATGGCGTCCAACCCCGAACGGGGGGAGATTCTGCTCACGGAACTGCAGGGG
GATTCCCGAAGTCTTCCGTTTTCTGAGAATGTGAGTGCTGTTCAAAAATTAGACTTTTCA
GATACAATGGTGCAGCAGAAATTGGATGATATCAAGGATCGAATTAAGAGAGAAATAAGG
AAAGAACTGAAAATCAAAGAAGGAGCTGAAAATCTGAGGAAAGTCACAACAGATAAAAAA
AGTTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAGAAGAACTACAT
CACAAGCTGCAGGAATTAAATGCACATATTGTTGTATCAGATCCAGAAGATATTACAGAT
TGCCCAAGGACTCCAGATACTCCAAATAATGACCCTCGTTGTTCTACTAGCAACAATAGA
TTGAAGGCCTTACAAAAACAATTGGATATAGAACTTAAAGTAAAACAAGGTGCAGAGAAT
ATGATACAGATGTATTCAAATGGATCTTCAAAGGATCGGAAACTCCATGGTACAGCTCAG
CAACTGCTCCAGGACAGCAAGACAAAAATAGAAGTCATACGAATGCAGATTCTTCAGGCA
GTCCAGACTAATGAATTGGCTTTTGATAATGCAAAACCTGTGATAAGTCCTCTTGAACTT
CGGATGGAAGAATTAAGGCATCATTTTAGGATAGAGTTTGCAGTAGCAGAAGGTGCAAAG
AATGTAATGAAATTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCACTTTCAGAAGCT
CAAGCAAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCATTAGAGCAA
AGATTAAACGAAGTCCCCAAGAATCATCCCAAAAGCAGGATTATTATTGAAGAACTTTCA
CTTGTTGCTGCATCACCAACACTAAGTCCACGTCAAAGTATGATATCTACGCAAAATCAA
TATAGTACACTATCCAAACCAGCAGCACTAACAGGTACTTTGGAAGTTCGTCTTATGGGC
TGCCAAGATATCCTAGAGAATGTCCCTGGACGGTCAAAAGCAACATCAGTTGCACTGCCT
GGTTGGAGTCCAAGTGAAACCAGATCATCTTTCATGAGCAGAACGAGTAAAAGTAAAAGC
GGAAGTAGTCGAAATCTTCTAAAAACCGATGACTTGTCCAATGATGTCTGTGCTGTTTTG
AAGCTCGATAATACTGTGGTTGGCCAAACTAGCTGGAAACCCATTTCCAATCAGTCATGG
GACCAGAAGTTTACACTGGAACTGGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGG
CGTGATTGGCGGTCTCTGTGTGCTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAAC
CAACGGCATGGCATGTGTCTCTATTTGGAACCACAGGGTACTTTATTTGCAGAGGTTACC
TTTTTTAATCCAGTTATTGAAAGAAGACCAAAACTTCAAAGACAAAAGAAAATTTTTTCA
AAGCAACAAGGCAAAACATTTCTCAGAGCTCCTCAAATGAATATTAATATTGCCACTTGG
GGAAGGCTAGTAAGAAGAGCTATTCCTACAGTAAATCATTCTGGCACCTTCAGCCCTCAA
GCTCCTGTGCCTACTACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTCCA
GCTAGTGATTCTACAGTAACCAAATTGGACTTTGATCTTGAGCCTGAACCTCCTCCAGCC
CCACCACGAGCTTCTTCTCTTGGAGAAATAGATGAATCTTCTGAATTAAGAGTTTTGGAT
ATACCAGGACAGGATTCAGAGACTGTTTTTGATATTCAGAATGACAGAAATAGTATACTT
CCAAAATCTCAATCTGAATACAAGCCTGATACTCCTCAGTCAGGCCTAGAATATAGTGGT
ATTCAAGAACTTGAGGACAGAAGATCTCAGCAAAGGTTTCAGTTTAATCTACAAGATTTC
AGGTGTTGTGCTGTCTTGGGAAGAGGACATTTTGGAAAGGTGCTTTTAGCTGAATATAAA
AACACAAATGAGATGTTTGCTATAAAAGCCTTAAAGAAAGGAGATATTGTGGCTCGAGAT
GAAGTAGACAGCCTGATGTGTGAAAAAAGAATTTTTGAAACTGTGAATAGTGTAAGGCAT
CCCTTTTTGGTGAACCTTTTTGCATGTTTCCAAACCAAAGAGCATGTTTGCTTTGTAATG
GAATATGCTGCCGGTGGGGACCTAATGATGCACATTCATACTGATGTCTTTTCTGAACCA
AGAGCTGTATTTTATGCTGCTTGTGTAGTTCTTGGGTTGCAGTATTTACATGAACACAAA
ATTGTTTATAGAGATTTGAAATTGGATAACTTATTGCTAGATACAGAGGGCTTTGTGAAA
ATTGCTGATTTTGGTCTTTGCAAAGAAGGAATGGGATATGGAGATAGAACAAGCACATTT
TGTGGCACTCCTGAATTTCTTGCCCCAGAAGTATTAACAGAAACTTCTTATACAAGGGCT
GTAGATTGGTGGGGCCTTGGCGTGCTTATATATGAAATGCTTGTTGGTGAGTCTCCCTTT
CCTGGTGATGATGAAGAGGAAGTTTTTGACAGTATTGTAAATGATGAAGTAAGGTATCCA
AGGTTCTTATCTACAGAAGCCATTTCTATAATGAGAAGGCTGTTAAGAAGAAATCCTGAA
CGGCGCCTTGGGGCTAGCGAGAAAGATGCAGAGGATGTAAAAAAGCACCCATTTTTCCGG
CTAATTGATTGGAGCGCTCTGATGGACAAAAAAGTAAAGCCACCATTTATACCTACCATA
AGAGGACGAGAAGATGTTAGTAATTTTGATGATGAATTTACCTCAGAAGCACCTATTCTG
ACTCCACCTCGAGAACCAAGGATACTTTCGGAAGAGGAGCAGGAAATGTTCAGAGATTTT
GACTACATTGCTGATTGGTGTTAAGTTGCTAGACACTGCGAAACCAAGCTGACTCACAAG
AAGACCTCTTAAAAATAGCAACCCTTCATTTGCTCTCTGTGCCACCAATAGCTTCTGAGT
TTTTTGTTGTTGTTGTTTTTATTGAAACACGTGAAGATTTGTTTAAAAGTACCATTCTAA
TACTTCTTCAAAAGTGGCTCCTCATTGTACTTCAGCGTAAATATGAGCACTGGAAACAGT
TTCATGGAGTTTAAGTTGAGTGAACATCGGCCATGAAAATCCATCACGAATACTTTTGGA
TCAATAGTCTATTTT
Protein sequence predicted based on nucleotide sequence
MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKEL KIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPR TPDTPNNDPRCSTSNNRLKALQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLL QDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFRIEFAVAEGAKNVM KLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNEVPKNHPKSRIIIEELSLVA ASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWS PSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQK FTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFN PVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPV PTTVPVVDVRIPQLAPPASDSTVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPG QDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCC AVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFL VNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVY RDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDW WGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRL GASEKDAEDVKKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPP REPRILSEEEQEMFRDFDYIADWC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
