Gene:
Gene Symbol:
ITK
Gene Name:
IL2-inducible T-cell kinase
Original Clone ID:
FLH232232.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618020
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1863nts
Open reading frame: 83 to 1945
TGCATTCTTTGCCCCAAAACTCTTTCCTTTGGTTGTGCTAAGAGGTGATGCCCAAGGTGC
ACCACCTTTCAAGAACTGGATCATGAACAACTTTATCCTCCTGGAAGAACAGCTCATCAA
GAAATCCCAACAAAAGAGAAGAACTTCTCCCTCGAACTTTAAAGTCCGCTTCTTCGTGTT
AACCAAAGCCAGCCTGGCATACTTTGAAGATCGTCATGGGAAGAAGCGCACGCTGAAGGG
GTCCATTGAGCTCTCCCGAATCAAATGTGTTGAGATTGTGAAAAGTGACATCAGCATCCC
ATGCCACTATAAATACCCGTTTCAGGTGGTGCATGACAACTACCTCCTATATGTGTTTGC
TCCAGATCGTGAGAGCCGGCAGCGCTGGGTGCTGGCCCTTAAAGAAGAAACGAGGAATAA
TAACAGTTTGGTGCCTAAATATCATCCTAATTTCTGGATGGATGGGAAGTGGAGGTGCTG
TTCTCAGCTGGAGAAGCTTGCAACAGGCTGTGCCCAATATGATCCAACCAAGAATGCTTC
AAAGAAGCCTCTTCCTCCTACTCCTGAAGACAACAGGCGACCACTTTGGGAACCTGAAGA
AACTGTGGTCATTGCCTTATATGACTACCAAACCAATGATCCTCAGGAACTCGCACTGCG
GCGCAACGAAGAGTACTGCCTGCTGGACAGTTCTGAGATTCACTGGTGGAGAGTCCAGGA
CAGGAATGGGCATGAAGGATATGTACCAAGCAGTTATCTGGTGGAAAAATCTCCAAATAA
TCTGGAAACCTATGAGTGGTACAATAAGAGTATCAGCCGAGACAAAGCTGAAAAACTTCT
TTTGGACACAGGCAAAGAAGGAGCCTTCATGGTAAGGGATTCCAGGACTGCAGGAACATA
CACCGTGTCTGTTTTCACCAAGGCTGTTGTAAGTGAGAACAATCCCTGTATAAAGCATTA
TCACATCAAGGAAACAAATGACAATCCTAAGCGATACTATGTGGCTGAAAAGTATGTGTT
CGATTCCATCCCTCTTCTCATCAACTATCACCAACATAATGGAGGAGGCCTGGTGACTCG
ACTCCGGTATCCAGTTTGTTTTGGGAGGCAGAAAGCCCCAGTTACAGCAGGGCTGAGATA
CGGGAAATGGGTGATCGACCCCTCAGAGCTCACTTTTGTGCAAGAGATTGGCAGTGGGCA
ATTTGGGTTGGTGCATCTGGGCTACTGGCTCAACAAGGACAAGGTGGCTATCAAAACCAT
TCGGGAAGGGGCTATGTCAGAAGAGGACTTCATAGAGGAGGCTGAAGTAATGATGAAACT
CTCTCATCCCAAACTGGTGCAGCTGTATGGGGTGTGCCTGGAGCAGGCCCCCATCTGCCT
GGTGTTTGAGTTCATGGAGCACGGCTGCCTGTCAGATTATCTACGCACCCAGCGGGGACT
TTTTGCTGCAGAGACCCTGCTGGGCATGTGTCTGGATGTGTGTGAGGGCATGGCCTACCT
GGAAGAGGCATGTGTCATCCACAGAGACTTGGCTGCCAGAAATTGTTTGGTGGGAGAAAA
CCAAGTCATCAAGGTGTCTGACTTTGGGATGACAAGGTTCGTTCTGGATGATCAGTACAC
CAGTTCCACAGGCACCAAATTCCCGGTGAAGTGGGCATCCCCAGAGGTTTTCTCTTTCAG
TCGCTATAGCAGCAAGTCCGATGTGTGGTCATTTGGTGTGCTGATGTGGGAAGTTTTCAG
TGAAGGCAAAATCCCGTATGAAAACCGAAGCAACTCAGAGGTGGTGGAAGACATCAGTAC
CGGATTTCGGTTGTACAAGCCCCGGCTGGCCTCCACACACGTCTACCAGATTATGAATCA
CTGCTGGAAAGAGAGACCAGAAGATCGGCCAGCCTTCTCCAGACTGCTGCGTCAACTGGC
TGAAATTGCAGAATCAGGACTTTAGTAGAGACTGAGTACCAGGCCACGGGCTGCAGATCC
TGAATGGAGGAAGGATATGTCCTCATTCCATAGAGCATTAGAAGCTGCCACCAGCCCAGG
ACCCTCCAGAGGCAGCCTGGCCTGTGGCATCAGTCCCTGAGTCACCATGGAAGCAGCATC
CTGACCACAGCTGGCAGTCAAGCCACAGCTGGAGGGTCAGCCACCAAGCTGGGAGCTGAG
CCAGAACAGGAGTGATGTCTCTGCCCTTCCTCTAGCCTCTTGTCACATGTGGTGCACAAA
CCTCAACCTGACAGCTTTCAGACAGCATTCTTGCACTTCTTAGCAACAGAGAGAGACATG
AGTAAGACCCAGATTGCTATTTTTATTGTTATTTTTAACATGAATCTAAAGNTTATGGTT
CCAGGGACTTTTTATTTGACCCAACAACACAGTATCCCAGGATATGGAGGCAAGGGGAAC
AAAGAGCATGAGTCTTTTTCCAAGAAAACTGGTGAGTTAAGTAAGATTAGAGTGAGTGTG
CTCTGTTGCTGTGATGCTGTCAGCCACAGCTTCCTGCCGTAGAGAATGATAGAGCAGCTG
CTCACACAGGAGGCCGGATATTCTGAGAAGCAGCTTTATGAGGTTTTACAGAGTATGCTG
CTACCTCTCTCCTTGAAGGGAGCATGGCGAGACCCATTGGATGGATTGGGGTGAACAGTT
CAGGTCCCATGCTTGGAGCATTGGGTATCTGATGTCTGCACCAGAACAAGAGAACCTCTG
ACGGTGGAGAACCATGTGGTGCAAGAAGAGATCTTAGGTCTCTTCTTTTATACCAAGCTC
ATCTTTTATACCAAGCTGTGCAGGTGACTATGCCTCCTCTTCTGCACAGAATGCTTCCAC
CAGCATCCTGAGAAGAAATGATTACTTCTGAAAAACATCCTTTTTTCCAGCCTCTGGGAA
TCAGCCCCCCCTCTCTGCACTATCCGATCCTCATCAACAGAGGGCAGCATTGTGTTGGTC
AATGTTCCCTTGGCGAGCAATTGAAACTTGTTTAGGCCCTAGGGTTGAGCAATTTTAAGG
TTGAGACTCCAAGTCTCCTAAAATTCTAGGAGAGAAATAAAGAGTCTGTTTTTGCTCAAA
CCATCAGGATGGAAACAGTCAGGCACTGACTGGGGTGCTTCCAAGAGGCATGAGAGTGCC
TACTCTGGCTTGAGCACTTCTATATGCAAGGTGAATATGTACTGAGCTAGGAGACTTCCC
TGCAAAATCTCTGTTCACCCTGGGTTCACATCCCCATGAGGTAATATTATTATTCCCATT
TTACAAATAATGTAACTGAGGCTTTAAAAAGCCAAGACATCTGCCCAAAGTGATGGAACT
AGAAAGTCTAGAGCTGGTATTCTAGCCCAAATCTGTCTGACCGCAATACACAGATTCTTT
ATTCCTATTCGACACTGGCTTCTACTGAAAATGAAACGGATTGCAGAGGGAATAAATACA
AAGATGGAAAGCCAGTAAAGAAGTCAGTATAGAACCACTAGCGAATAGTGTTGCTCTGGC
ACAGACCACTGTGGTTGATGGCATGGCCCTCCAACTTGGAATAGGATTTTCCTTTTCCTA
TTCTGTATCCTTACCTTGGTCATGTTAATGACTTTGGAGTTATTCAGTTAATGACCCTTT
AATTCTCACAACCAACCAGTCATGTTGCTTGAAGCCATTTATAGACGAGCTTCAAAGCAA
CTTTAAAAGATTCTTCTGTAGAAGTATGAGTTCTTCCTTTAATTATCATTCCAACTTTCA
GCTGTAGTCTTCTTGAACACTTCATGAGGAGGGACATTCCCTGATATAAGAGAGGATGGT
GTTGCAATTGGCTCTTTCTAAATCATGTGACGTTTTGACTGGCTTGAGATTCAGATGCAT
AATTTTTAATTATAATTATTGTGAAGTGGAGAGCCTCAAGATAAAACTCTGTCATTCAGA
AGATGATTTTACTCAGCTTATCCAAAATTATCTCTGTTTACTTTTTAGAATTTTGTACAT
TATCTTTTGGGATCCTTAATTAGAGATGATTTCTGGAACATTCAGTCTAGAAAGAAAACA
TTGGAATTGACTGATCTCTGTGGTTTGGTTTAGAAAATTCCCCTGTGCATGGTATTACCT
TTTTCAAGCTCAGATTCATCTAATCCTCAACTGTACATGTGTACATTCTTCACCTCCTGG
TGCCCTATCCCGCAAAATGGGCTTCCTGCCTGGTTTTTCTCTTCTCACATTTTTTAAATG
GTCCCCTGTGTTTGTAGAGAACTCCCTTATACAGAGTTTTGGTTCTAGTTTTATTTCGTA
GATTTTGCATTTTGTACCTTTTGAGACTATGTATTTATATTTGGATCAGATGCATATTTA
TTAATGTACAGTCACTGCTAGTGTTCAAAATAAAAATGTTACAAAT
Protein sequence predicted based on nucleotide sequence
MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRI KCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKY HPNFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPEETVVIALY DYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWY NKSISRDKAEKLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETND NPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDP SELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQ LYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIH RDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSD VWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPE DRPAFSRLLRQLAEIAESGL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
