DNASU Plasmid | PRKCE (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: PRKCE

Gene Name: protein kinase C, epsilon

Original Clone ID:

HsCD00298656

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00618083

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2214nts

Open reading frame: 198 to 2411

GAACCCGGCGAGGAAATACATGCACTGGCTGAGAATCGCCCGCGCCAGGGCGCAACGCCA
CAAGGTGTAGGGAGTGTGCGGGGTGGGGCGAAAGGGGACCCAAGAGTCCCTGTGGCTCGG
AGTGCCGGGCCGTCGGTTCTTCATTCCTGCCCTCGGGGCAGACGGAGTGACCCCGGCCCC
CACTCCCCGCCCCGACCATGGTAGTGTTCAATGGCCTTCTTAAGATCAAAATCTGCGAGG
CCGTGAGCTTGAAGCCCACAGCCTGGTCGCTGCGCCATGCGGTGGGACCCCGGCCGCAGA
CTTTCCTTCTCGACCCCTACATTGCCCTCAATGTGGACGACTCGCGCATCGGCCAAACGG
CCACCAAGCAGAAGACCAACAGCCCGGCCTGGCACGACGAGTTCGTCACCGATGTGTGCA
ACGGACGCAAGATCGAGCTGGCTGTCTTTCACGATGCCCCCATAGGCTACGACGACTTCG
TGGCCAACTGCACCATCCAGTTTGAGGAGCTGCTGCAGAACGGGAGCCGCCACTTCGAGG
ACTGGATTGATCTGGAGCCAGAAGGAAGAGTGTATGTGATCATCGATCTCTCAGGGTCGT
CGGGTGAAGCCCCTAAAGACAATGAAGAGCGTGTGTTCAGGGAACGCATGCGGCCGAGGA
AGCGGCAGGGGGCCGTCAGGCGCAGGGTCCATCAGGTCAACGGCCACAAGTTCATGGCCA
CCTATCTTCGGCAGCCCACCTACTGCTCCCATTGCAGAGACTTCATCTGGGGTGTCATAG
GAAAGCAGGGATACCAGTGTCAAGTCTGCACCTGCGTGGTCCACAAGCGGTGCCACGAGC
TCATAATCACAAAGTGTGCTGGGTTAAAGAAGCAGGAGACCCCCGACCAGGTGGGCTCCC
AGCGGTTCAGCGTCAACATGCCCCACAAGTTCGGTATCCACAACTACAAGGTCCCTACCT
TCTGCGATCACTGTGGGTCCCTGCTCTGGGGACTCTTGCGGCAGGGTTTGCAGTGTAAAG
TCTGCAAAATGAATGTTCACCGTCGATGTGAGACCAACGTGGCTCCCAACTGTGGAGTGG
ATGCCAGAGGAATCGCCAAAGTACTGGCCGACCTGGGCGTTACCCCAGACAAAATCACCA
ACAGCGGCCAGAGAAGGAAAAAGCTCATTGCTGGTGCCGAGTCCCCGCAGCCTGCTTCTG
GAAGCTCACCATCTGAGGAAGATCGATCCAAGTCAGCACCCACCTCCCCTTGTGACCAGG
AAATAAAAGAACTTGAGAACAACATTCGGAAAGCCTTGTCATTTGACAACCGAGGAGAGG
AGCACCGGGCAGCATCGTCTCCTGATGGCCAGCTGATGAGCCCCGGTGAGAATGGCGAAG
TCCGGCAAGGCCAGGCCAAGCGCCTGGGCCTGGATGAGTTCAACTTCATCAAGGTGTTGG
GCAAAGGCAGCTTTGGCAAGGTCATGTTGGCAGAACTCAAGGGCAAAGATGAAGTATATG
CTGTGAAGGTCTTAAAGAAGGACGTCATCCTTCAGGATGATGACGTGGACTGCACAATGA
CAGAGAAGAGGATTTTGGCTCTGGCACGGAAACACCCGTACCTTACCCAACTCTACTGCT
GCTTCCAGACCAAGGACCGCCTCTTTTTCGTCATGGAATATGTAAATGGTGGAGACCTCA
TGTTTCAGATTCAGCGCTCCCGAAAATTCGACGAGCCTCGTTCACGGTTCTATGCTGCAG
AGGTCACATCGGCCCTCATGTTCCTCCACCAGCATGGAGTCATCTACAGGGATTTGAAAC
TGGACAACATCCTTCTGGATGCAGAAGGTCACTGCAAGCTGGCTGACTTCGGGATGTGCA
AGGAAGGGATTCTGAATGGTGTGACGACCACCACGTTCTGTGGGACTCCTGACTACATAG
CTCCTGAGATCCTGCAGGAGTTGGAGTATGGCCCCTCCGTGGACTGGTGGGCCCTGGGGG
TGCTGATGTACGAGATGATGGCTGGACAGCCTCCCTTTGAGGCCGACAATGAGGACGACC
TATTTGAGTCCATCCTCCATGACGACGTGCTGTACCCAGTCTGGCTCAGCAAGGAGGCTG
TCAGCATCTTGAAAGCTTTCATGACGAAGAATCCCCACAAGCGCCTGGGCTGTGTGGCAT
CGCAGAATGGCGAGGACGCCATCAAGCAGCACCCATTCTTCAAAGAGATTGACTGGGTGC
TCCTGGAGCAGAAGAAGATCAAGCCACCCTTCAAACCACGCATTAAAACCAAAAGAGACG
TCAATAATTTTGACCAAGACTTTACCCGGGAAGAGCCGGTACTCACCCTTGTGGACGAAG
CAATTGTAAAGCAGATCAACCAGGAGGAATTCAAAGGTTTCTCCTACTTTGGTGAAGACC
TGATGCCCTGAGAGCCCACTGCAGTTGGACTTTGCCGATGCTGCAAGAAGGGGTGCAGAG
AAGACTCCTGTGTTGGAGACACTCAGCAGGTCTTGAACTACTTCTCCTCCTCGGAGCCCC
AGTCCCATGTCCACTGTCTATTTATTGCATTCCCTTGCCCCAGGCCACCTCCTCCCCCTC
CCACCTGGTGACCAGAAGGCGCTCTCGGTTCTTGTCTCACCAGTAATGCAGACTCATTGG
GTCAGCAATTAGCTGTATACACTGCCGTGTTTGGACCATTGGCAAGCCTGGTTCCACTCC
TCAGGGGCTCCTGGCAGTGAAGCAACTTCAGTTCTTTTACTGCAAAGAACAGAAAAAAGA
AAGAAAGCAAACAAGAAGACTCCGGCTCTGCTATCGGACACAGATCCTGATCCCTCTTGC
TTCTTTTCCCTCCTGCACCGCAGCTTGCCATCCCTGCCCTTCTGTCCTGGAGAAGAGACT
GGTGCTTCTCCGCACACACGAGGGAGGGCGCCCTTGAGGCATGCCCTCTGAGGGAGGGAG
ACCAGAGATGCAGGGATTGGCCAGCTGGGTTGGTTTGCTCTGGAATGGCTAACTCTTGCC
TGCTTTGGTTTTAGCTTTTCAGCATGCCAAAGTCATGTAAGTTTGTGTCTTGTGGAAGAA
ATCCTCTTTGTGGAAAAAGAAACAGGGTTTTGAACTCTGTTAACATTTGAAAAATATATT
TTCAAATTCACTTTCTAATTGGCCAAAAGAGATGAGTTCCAGTCTGAATACAGGTAGATA
TTAAAGGGCTAATAAAAAATGAGAAACCGGTCGTCCAAGGTGGATGCTGTCAATGCCCGA
GTGACACATGAGAGCTGTATGAATTGAGAGAAAAGGCAACAAGTAGCATTCTTCATCATT
CAAGTTCTACCTGGACACAAAGGCGAGGACCCTGGGGTTCCAACAAAGCTCAGCTCCCAG
ATTCTCTTTCCAGTTTCATCCTAAGTTCCTAGCATAAACACTATTTATTTTCTGCAGCAG
TGTGTTATTTTTGCGCACTTATACAAAATGGTAGTACTACTGTGTTGTGGTTTTTAAACA
TTAAACATGTAAAGTTATATACGAAATATCTGCTTTTGGAATAAGCAGAATGAGGCTAAA
CATGGGTTATACAAAGGGTATCTGGAAACTGAAGAGCAACTTGTTAGAAAACTGACAATG
TCGCAAGATGTACTCAGTTTTGTTTCTGTGTGACATGCAATGGCAACTCATGTGGACACT
ATTGAAGGGATGTGACATTACCTCCTGTAGATATGCTAACAGTGTTATTCTTTCATTTCC
AAGGGTTCTCTGTGGCTTTGTGTATATGTTTCCCAGAGGTCATTTGATTACCTAATTTAC
TGAACTGATTTAGCAGGGAATGGAATCCATTCCAACTATTGCACGTGGATTTCCCAGCTG
CCCCTAAATATATATACTTGTGAGTGGCAAAGTGGCACTAATGAAGCTTTTGCCTTTTGT
ACATTTGAGATTTTTGTATATAGTGTTTGCTGCAAGGCCTGTGGAATTAATTCGTTGCAT
ATAGAGGTATCAACTGCTGCATGTTCAGGCATATTATAAAACTTTAGTCTATGAAAGAAT
AATTATAATAATGTCCAGGTGCAATACTCTGTAAGTCTATTGGTTCAAGTTACCGAGAGA
TAGGTGTGTTCCTTTATGGGGGATGGGGGGGTGTGTTGGGGATTCTTTGTATTGTTTATT
TCATTTTGGTTTATTTTAAAAGATGTAAACATATATTAAGCTATATTAAATCTCACATAC
AGTTCTTCTGTGCTCTATTATACCCTGATAGAGATGGGGGAGAGAAAGGAATGTTTTTGA
TGGTGGTTTCAAAGCTCGGACAGTAACTATCTTGAGCCCATTAGAGAGTCTGTGTCCATA
TTTGCATCTGGCTGGTCATAGCCTTTGTTACTAATGATGACATTCAGTTCTCTTTTGTTT
TTATTTTTTAAAAACTCAGGTGTAATTATTATCTGTTCTTAAGATAATTGCAAATATTAA
ATATTATGATATATCAATTCATGTGTTTGGCATACCAGTGAATGATGAAGAACATGAGAT
TAATTTAATTTATCTTCGGTAACTTGACATTCTGGAGAGAGACTATCTTCTGGAGTTGAG
TACAAGCACAGAAACATCTTTACGGTGGCATCATCTCATTTTTTAGGAAGACATGATAAT
ACTGCCCATCATATTCATGTGTAACTACTGTTCTTTCTTCTGCTTTCTTCACCATAATAA
ACTTTGGACAACCAAGCAAGCTCTAACCGCAATGCCAGATGGCCTTGTCCGAGGGCCTAG
TGTTTGCACGGCAGTGGGAACTGGGCCTTTCCTACAGGACAACTGGCAAGTTTGCTGGGA
AGTCAAATAATACATTCCACCTGGCAGCTGAAGGCAGCCAGTCAGTCTGTCCCAGAAAGG
GCCCTTTTCAGCACCCAAAGCTGGGCTGGCTGGGATGCCTCTGGCTGGTGAAGTTCTCAC
ATAGGCTGATTTAAATCCAGCAAAGGTCTATAGAAAAAGGCTTGCGTGTTCGTTGAGTAA
TCATTGTTTCATTTTCATTTTTACGAGAGTTTGAAAATAGACACACTGTTAACACTTCTG
CCAGTTTTTTCTGATCTTTCCAGCCCCACCCCCTTTCTCTTTCTCTCTCTCTCTCAAAGA
AAAAAAAAATGGGAGTGCAAAAAAAACAAAGCCAAAAAATATATGAAGGATAGCTGTTCT
TCTGTGTTCTCTCATTATGGACTTTGTGAAGTAGAAACATAATTTTTTTTCCTCCAAAGG
TGAAAAAACAATGCATTCTTGCTTTAAAAAAAAAAAAGAAGGCTAAAAAATTACCTCTTT
TTAAATTATGTGCAAAATAATTCTGGCTAACTGTAAAATGTATTCAATTTTAGGATTTTT
TTTTTTTGTATTGTGATGCTTTATTTGTACATTTTTTTCCTTTCTGGATGTAATTTTAAT
CTCTTGCCATTCATTAGTGTTATTTCATTGTAAACGTTATTGTGCCAAATGTACTGTATT
CAAAAGGATGTGAATGTGTATTGTTTCAGAACCTAATAAATACAATGACGTTAAGTCTTA
AAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKT
NSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLE
PEGRVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQP
TYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDQVGSQRFSVN
MPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIA
KVLADLGVTPDKITNSGQRRKKLIAGAESPQPASGSSPSEEDRSKSAPTSPCDQEIKELE
NNIRKALSFDNRGEEHRAASSPDGQLMSPGENGEVRQGQAKRLGLDEFNFIKVLGKGSFG
KVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKD
RLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILL
DAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEM
MAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVASQNGED
AIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQI
NQEEFKGFSYFGEDLMP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

PRKCE

Symbol Nomenclature :

PRKCE

SYNONYM :

PKCE; nPKC-epsilon

Designation :

protein kinase C epsilon type

Full Nomenclature :

protein kinase C, epsilon

GENEID :

5581

HGNC :

9401

MIM :

176975

Vega :

OTTHUMG00000128817

Target GenBank:

NM_005400

Pathways

NCI :

CDC42 signaling events

NCI :

Downstream signaling in naïve CD8+ T cells

NCI :

Endothelins

NCI :

IL2-mediated signaling events

NCI :

IL8- and CXCR1-mediated signaling events

NCI :

LPA receptor mediated events

NCI :

LPA4-mediated signaling events

NCI :

PDGFR-beta signaling pathway

NCI :

Regulation of Ras family activation

NCI :

Role of Calcineurin-dependent NFAT signaling in lymphocytes

NCI :

TCR signaling in naïve CD4+ T cells

NCI :

TCR signaling in naïve CD8+ T cells

NCI :

Thromboxane A2 receptor signaling

Panther :

5HT2 type receptor mediated signaling pathway

Panther :

Alpha adrenergic receptor signaling pathway

Panther :

Alzheimer disease-amyloid secretase pathway

Panther :

Angiogenesis

Panther :

Apoptosis signaling pathway

Panther :

EGF receptor signaling pathway

Panther :

Endothelin signaling pathway

Panther :

FGF signaling pathway

Panther :

Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway

Panther :

Histamine H1 receptor mediated signaling pathway

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Panther :

Muscarinic acetylcholine receptor 1 and 3 signaling pathway

Panther :

Oxytocin receptor mediated signaling pathway

Panther :

Thyrotropin-releasing hormone receptor signaling pathway

Panther :

VEGF signaling pathway

Panther :

Wnt signaling pathway

Reactome :

DAG and IP3 signaling

Reactome :

DAP12 interactions

Reactome :

DAP12 signaling

Reactome :

Disease

Reactome :

Downstream signal transduction

Reactome :

Downstream signaling of activated FGFR

Reactome :

Effects of PIP2 hydrolysis

Reactome :

EGFR interacts with phospholipase C-gamma

Reactome :

Fcgamma receptor (FCGR) dependent phagocytosis

Reactome :

G alpha (q) signalling events

Reactome :

G alpha (z) signalling events

Reactome :

Gastrin-CREB signalling pathway via PKC and MAPK

Reactome :

GPCR downstream signaling

Reactome :

Hemostasis

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

Phospholipase C-mediated cascade

Reactome :

Platelet activation, signaling and aggregation

Reactome :

PLC-gamma1 signalling

Reactome :

PLCG1 events in ERBB2 signaling

Reactome :

Role of phospholipids in phagocytosis

Reactome :

Signal Transduction

Reactome :

Signaling by EGFR

Reactome :

Signaling by EGFR in Cancer

Reactome :

Signaling by ERBB2

Reactome :

Signaling by FGFR

Reactome :

Signaling by FGFR in disease

Reactome :

Signaling by GPCR

Reactome :

Signaling by PDGF

Reactome :

Signalling by NGF

WikiPathway :

Calcium Regulation in the Cardiac Cell

WikiPathway :

G Protein Signaling Pathways

WikiPathway :

Hypothetical Network for Drug Addiction

WikiPathway :

miRs in Muscle Cell Differentiation

WikiPathway :

Myometrial Relaxation and Contraction Pathways

WikiPathway :

Physiological and Pathological Hypertrophy of the Heart

WikiPathway :

Wnt Signaling Pathway

WikiPathway :

Wnt Signaling Pathway and Pluripotency

Protein

SMART domain :

C1 : Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

SMART domain :

C2 : Protein kinase C conserved region 2 (CalB)

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TK_X : Extension to Ser/Thr-type protein kinases

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

Q02156

HPRD :

01500

Clone

Cloning Information :

649611

HIP Master Clone ID :

148215

Original Clone ID :

FLH230794.01L

Original Clone ID :

HsCD00299172

Original Clone ID :

FLH232165.01L

Original Clone ID :

HsCD00298656

Organism

Species Specific ID:

5581

Genome

Chromosome :

Map Location :

2p21

Ensembl :

ENSG00000171132

Reagents

Labome :

PKC-epsilon-antibody

PRKCE (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

PRKCE (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)