Gene:
Gene Symbol:
PFKFB3
Gene Name:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Original Clone ID:
HsCD00299201
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618085
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1563nts
Open reading frame: 330 to 1892
AGCCAAGCCGGAGAGGAGGCGAGCGGCAGGGCCTGGTGGCGAGAGCGCGGCTGTCACTGC
GCCCGAGCATCCCAGAGCTTTCCGAGCGGACGAGCCGGCCGTGCCGGGCATCCCCAGCCT
CGCTACCCTCGCAGCACACGTCGAGCCCCGCACAGGCGAGGGTCCGGAACTTAGCCCAAA
GCACGTTTCCCCTGGCAGCGCAGGAAACGCCCGGCCGCGCGCCGGCGCACGCCCCCCTCT
CCTCCTTTGTTCCGGGGGTCGGCGGCCGCTCTCCTGCCAGCGTCGGGATCTCGGCCCCGG
GAGGCGGGCCGTCGGGCGCAGCCGCGAAGATGCCGTTGGAACTGACGCAGAGCCGAGTGC
AGAAGATCTGGGTGCCCGTGGACCACAGGCCCTCGTTGCCCAGATCCTGTGGGCCAAAGC
TGACCAACTCCCCCACCGTCATCGTCATGGTGGGCCTCCCCGCCCGGGGCAAGACCTACA
TCTCCAAGAAGCTGACTCGCTACCTCAACTGGATTGGCGTCCCCACAAAAGTGTTCAACG
TCGGGGAGTATCGCCGGGAGGCTGTGAAGCAGTACAGCTCCTACAACTTCTTCCGCCCCG
ACAATGAGGAAGCCATGAAAGTCCGGAAGCAATGTGCCTTAGCTGCCTTGAGAGATGTCA
AAAGCTACCTGGCGAAAGAAGGGGGACAAATTGCGGTTTTCGATGCCACCAATACTACTA
GAGAGAGGAGACACATGATCCTTCATTTTGCCAAAGAAAATGACTTTAAGGCGTTTTTCA
TCGAGTCGGTGTGCGACGACCCTACAGTTGTGGCCTCCAATATCATGGAAGTTAAAATCT
CCAGCCCGGATTACAAAGACTGCAACTCGGCAGAAGCCATGGACGACTTCATGAAGAGGA
TCAGTTGCTATGAAGCCAGCTACCAGCCCCTCGACCCCGACAAATGCGACAGGGACTTGT
CGCTGATCAAGGTGATTGACGTGGGCCGGAGGTTCCTGGTGAACCGGGTGCAGGACCACA
TCCAGAGCCGCATCGTGTACTACCTGATGAACATCCACGTGCAGCCGCGTACCATCTACC
TGTGCCGGCACGGCGAGAACGAGCACAACCTCCAGGGCCGCATCGGGGGCGACTCAGGCC
TGTCCAGCCGGGGCAAGAAGTTTGCCAGTGCTCTGAGCAAGTTCGTGGAGGAGCAGAACC
TGAAGGACCTGCGCGTGTGGACCAGCCAGCTGAAGAGCACCATCCAGACGGCCGAGGCGC
TGCGGCTGCCCTACGAGCAGTGGAAGGCGCTCAATGAGATCGACGCGGGCGTCTGTGAGG
AGCTGACCTACGAGGAGATCAGGGACACCTACCCTGAGGAGTATGCGCTGCGGGAGCAGG
ACAAGTACTATTACCGCTACCCCACCGGGGAGTCCTACCAGGACCTGGTCCAGCGCTTGG
AGCCAGTGATCATGGAGCTGGAGCGGCAGGAGAATGTGCTGGTCATCTGCCACCAGGCCG
TCCTGCGCTGCCTGCTTGCCTACTTCCTGGATAAGAGTGCAGAGGAGATGCCCTACCTGA
AATGCCCTCTTCACACCGTCCTGAAACTGACGCCTGTCGCTTATGGCTGCCGTGTGGAAT
CCATCTACCTGAACGTGGAGTCCGTCTGCACACACCGGGAGAGGTCAGAGGATGCAAAGA
AGGGACCTAACCCGCTCATGAGACGCAATAGTGTCACCCCGCTAGCCAGCCCCGAACCCA
CCAAAAAGCCTCGCATCAACAGCTTTGAGGAGCATGTGGCCTCCACCTCGGCCGCCCTGC
CCAGCTGCCTGCCCCCGGAGGTGCCCACGCAGCTGCCTGGACAAAACATGAAAGGCTCCC
GGAGCAGCGCTGACTCCTCCAGGAAACACTGAGGCAGACGTGTCGGTTCCATTCCATTTC
CATTTCTGCAGCTTAGCTTGTGTCCTGCCCTCCGCCCGAGGCAAAACGTATCCTGAGGAC
TTCTTCCGGAGAGGGTGGGGTGGAGCAGCGGGGGAGCCTTGGCCGAAGAGAACCATGCTT
GGCACCGTCTGTGTCCCCTCGGCCGCTGGACACCAGAAAGCCACGTGGGTCCCTGGCGCC
CTGCCTTTAGCCGTGGGGCCCCCACCTCCACCCTCTGGGTTTCCTAGGAATGTCCAGCCT
CGGAGACCTTCACAAAGCCTTGGGAGGGTGATGAGTGCTGGTCCTGACAAGAGGCCGCTG
GGGACACTGTGCTGTTTTGTTTCGTTTCTGTGATCTCCCGGCACGTTTGGAGCTGGGAAG
ACCACACTGGTGGCAGAATCCTAAAATTAAAGGAGGCAGGCTCCTAGTTGCTGAAAGTTA
AGGAATGTGTAAAACCTCCACGTGACTGTTTGGTGCATCTTGACCTGGGAAGACGCCTCA
TGGGAACGAACTTGGACAGGTGTTGGGTTGAGGCCTCTTCTGCAGGAAGTCCCTGAGCTG
AGACGCAAGTTGGCTGGGTGGTCCACACCCTGGCTCTCCTGCAGGTCCACACACCTTCCA
GGCCTGTGGCCTGCCTCCAAAGATGTGCAAGGGCAGGCTGGCTGCACGGGGAGAGGGAAG
TATTTTGCCGAAATATGAGAACTGGGGCCTCCTGCTCCCAGGGAGCTCCAGGGCCCCTCT
CTCCTCCCACCTGGACTTGGGGGGAACTGAGAAACACTTTCCTGGAGCTGCTGGCTTTTG
CACTTTTTTGATGGCAGAAGTGTGACCTGAGAGTCACACCTTCTCTTCAGGAACGTAGAT
GTTGGGGTGTCTTGCCCTGGGGGGCTTGGAACCTCTGAAGGTGGGGAGCGGAACACCTGG
CATCCTTCCCCAGCACTTGCATTACCGTCCCTGCTCTTCCCAGGTGGGGACAGTGGCCCA
AGCAAGGCCTCACTCGCAGCCACTTCTTCAAGAGCTGCCTGCACACTGTCTTGGAGCATC
TGCCTTGTGCCTGGCACTCTGCCGGTGCCTTGGGAAGGTCGGAAGAGTGGACTTTGTCCT
GGCCTTCCCTTCATGGCGTCTATGACACTTTTGTGGTGATGGAAAGCATGGGACCTGTCG
TCTCAGCCTGTTGGTTTCTCCTCATTGCCTCAAACCCTGGGGTAGGTGGGACGGGGGGTC
TCGTGCCCAGATGAAACCATTTGGAAACTCGGCAGCAGAGTTTGTCCAAATGACCCTTTT
CAGGATGTCTCAAAGCTTGTGCCAAAGGTCACTTTTCTTTCCTGCCTTCTGCTGTGAGCC
CTGAGATCCTCCTCCCAGCTCAAGGGACAGGTCCTGGGTGAGGGTGGGAGATTTAGACAC
CTGAAACTGGGCGTGGAGAGAAGAGCCGTTGCTGTTTGTTTTTTGGGAAGAGCTTTTAAA
GAATGCATGTTTTTTTCCTGGTTGGAATTGAGTAGGAACTGAGGCTGTGCTTCAGGTATG
GTACAATCAAGTGGGGGATTTTCATGCTGAACCATTCAAGCCCTCCCCGCCCGTTGCACC
CACTTTGGCTGGCGTCTGCTGGAGAGGATGTCTCTGTCCGCATTCCCGTGCAGCTCCAGG
CTCGCGCAGTTTTCTCTCTCTCCCTGGATGTTGAGTCTCATCAGAATATGTGGGTAGGGG
GTGGACGTGCACGGGTGCATGATTGTGCTTAACTTGGTTGTATTTTTCGATTTGACATGG
AAGGCCTGTTGCTTTGCTCTTGAGAATAGTTTCTCGTGTCCCCCTCGCAGGCCTCATTCT
TTGAACATCAACTCTGAAGTTTGATACAGATAGGGGCTTGATAGCTGTGGTCCCCTCTCC
CCTCTGACTACCTAAAATCAATACCTAAATACAGAAGCCTTGGTCTAACACGGGACTTTT
AGTTTGCGAAGGGCCTAGATAGGGAGAGAGGTAACATGAATCTGGACAGGGAGGGAGATA
CTATAGAAAGGAGAACACTGCCTACTTTGCAAGCCAGTGACCTGCCTTTTGAGGGGACAT
TGGACGGGGGCCGGGGGCGGGGGTTGGGTTTGAGCTACAGTCATGAACTTTTGGCGTCTA
CTGATTCCTCCAACTCTCCACCCCACAAAATAACGGGGACCAATATTTTTAACTTTGCCT
ATTTGTTTTTGGGTGAGTTTCCCCCCTCCTTATTCTGTCCTGAGACCACGGGCAAAGCTC
TTCATTTTGAGAGAGAAGAAAAACTGTTTGGAACCACACCAATGATATTTTTCTTTGTAA
TACTTGAAATTTATTTTTTTATTATTTTGATAGCAGATGTGCTATTTATTTATTTAATAT
GTATAAGGAGCCTAAACAATAGAAAGCTGTAGAGATTGGGTTTCATTGTTAATTGGTTTG
GGAGCCTCCTATGTGTGACTTATGACTTCTCTGTGTTCTGTGTATTTGTCTGAATTAATG
ACCTGGGATATAAAGCTATGCTAGCTTTCAAACAGGAGATGCCTTTCAGAAATTTGTATA
TTTTGCAGTTGCCAGACCAATAAAATACCTGGTTGAAATACAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLN WIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQ IAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNS AEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLM NIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQ LKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTG ESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKL TPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFE EHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
