Gene:
Gene Symbol:
EPHB2
Gene Name:
EPH receptor B2
Original Clone ID:
HsCD00299206
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618088
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2964nts
Open reading frame: 19 to 2982
GCCCCGGGAAGCGCAGCCATGGCTCTGCGGAGGCTGGGGGCCGCGCTGCTGCTGCTGCCG
CTGCTCGCCGCCGTGGAAGAAACGCTAATGGACTCCACTACAGCGACTGCTGAGCTGGGC
TGGATGGTGCATCCTCCATCAGGGTGGGAAGAGGTGAGTGGCTACGATGAGAACATGAAC
ACGATCCGCACGTACCAGGTGTGCAACGTGTTTGAGTCAAGCCAGAACAACTGGCTACGG
ACCAAGTTTATCCGGCGCCGTGGCGCCCACCGCATCCACGTGGAGATGAAGTTTTCGGTG
CGTGACTGCAGCAGCATCCCCAGCGTGCCTGGCTCCTGCAAGGAGACCTTCAACCTCTAT
TACTATGAGGCTGACTTTGACTCGGCCACCAAGACCTTCCCCAACTGGATGGAGAATCCA
TGGGTGAAGGTGGATACCATTGCAGCCGACGAGAGCTTCTCCCAGGTGGACCTGGGTGGC
CGCGTCATGAAAATCAACACCGAGGTGCGGAGCTTCGGACCTGTGTCCCGCAGCGGCTTC
TACCTGGCCTTCCAGGACTATGGCGGCTGCATGTCCCTCATCGCCGTGCGTGTCTTCTAC
CGCAAGTGCCCCCGCATCATCCAGAATGGCGCCATCTTCCAGGAAACCCTGTCGGGGGCT
GAGAGCACATCGCTGGTGGCTGCCCGGGGCAGCTGCATCGCCAATGCGGAAGAGGTGGAT
GTACCCATCAAGCTCTACTGTAACGGGGACGGCGAGTGGCTGGTGCCCATCGGGCGCTGC
ATGTGCAAAGCAGGCTTCGAGGCCGTTGAGAATGGCACCGTCTGCCGAGGTTGTCCATCT
GGGACTTTCAAGGCCAACCAAGGGGATGAGGCCTGTACCCACTGTCCCATCAACAGCCGG
ACCACTTCTGAAGGGGCCACCAACTGTGTCTGCCGCAATGGCTACTACAGAGCAGACCTG
GACCCCCTGGACATGCCCTGCACAACCATCCCCTCCGCGCCCCAGGCTGTGATTTCCAGT
GTCAATGAGACCTCCCTCATGCTGGAGTGGACCCCTCCCCGCGACTCCGGAGGCCGAGAG
GACCTCGTCTACAACATCATCTGCAAGAGCTGTGGCTCGGGCCGGGGTGCCTGCACCCGC
TGCGGGGACAATGTACAGTACGCACCACGCCAGCTAGGCCTGACCGAGCCACGCATTTAC
ATCAGTGACCTGCTGGCCCACACCCAGTACACCTTCGAGATCCAGGCTGTGAACGGCGTT
ACTGACCAGAGCCCCTTCTCGCCTCAGTTCGCCTCTGTGAACATCACCACCAACCAGGCA
GCTCCATCGGCAGTGTCCATCATGCATCAGGTGAGCCGCACCGTGGACAGCATTACCCTG
TCGTGGTCCCAGCCGGACCAGCCCAATGGCGTGATCCTGGACTATGAGCTGCAGTACTAT
GAGAAGGAGCTCAGTGAGTACAACGCCACAGCCATAAAAAGCCCCACCAACACGGTCACC
GTGCAGGGCCTCAAAGCCGGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCGTGGCA
GGCTACGGGCGCTACAGCGGCAAGATGTACTTCCAGACCATGACAGAAGCCGAGTACCAG
ACAAGCATCCAGGAGAAGTTGCCACTCATCATCGGCTCCTCGGCCGCTGGCCTGGTCTTC
CTCATTGCTGTGGTTGTCATCGCCATCGTGTGTAACAGAAGACGGGGGTTTGAGCGTGCT
GACTCGGAGTACACGGACAAGCTGCAACACTACACCAGTGGCCACATGACCCCAGGCATG
AAGATCTACATCGATCCTTTCACCTACGAGGACCCCAACGAGGCAGTGCGGGAGTTTGCC
AAGGAAATTGACATCTCCTGTGTCAAAATTGAGCAGGTGATCGGAGCAGGGGAGTTTGGC
GAGGTCTGCAGTGGCCACCTGAAGCTGCCAGGCAAGAGAGAGATCTTTGTGGCCATCAAG
ACGCTCAAGTCGGGCTACACGGAGAAGCAGCGCCGGGACTTCCTGAGCGAAGCCTCCATC
ATGGGCCAGTTCGACCATCCCAACGTCATCCACCTGGAGGGTGTCGTGACCAAGAGCACA
CCTGTGATGATCATCACCGAGTTCATGGAGAATGGCTCCCTGGACTCCTTTCTCCGGCAA
AACGATGGGCAGTTCACAGTCATCCAGCTGGTGGGCATGCTTCGGGGCATCGCAGCTGGC
ATGAAGTACCTGGCAGACATGAACTATGTTCACCGTGACCTGGCTGCCCGCAACATCCTC
GTCAACAGCAACCTGGTCTGCAAGGTGTCGGACTTTGGGCTCTCACGCTTTCTAGAGGAC
GATACCTCAGACCCCACCTACACCAGTGCCCTGGGCGGAAAGATCCCCATCCGCTGGACA
GCCCCGGAAGCCATCCAGTACCGGAAGTTCACCTCGGCCAGTGATGTGTGGAGCTACGGC
ATTGTCATGTGGGAGGTGATGTCCTATGGGGAGCGGCCCTACTGGGACATGACCAACCAG
GATGTAATCAATGCCATTGAGCAGGACTATCGGCTGCCACCGCCCATGGACTGCCCGAGC
GCCCTGCACCAACTCATGCTGGACTGTTGGCAGAAGGACCGCAACCACCGGCCCAAGTTC
GGCCAAATTGTCAACACGCTAGACAAGATGATCCGCAATCCCAACAGCCTCAAAGCCATG
GCGCCCCTCTCCTCTGGCATCAACCTGCCGCTGCTGGACCGCACGATCCCCGACTACACC
AGCTTTAACACGGTGGACGAGTGGCTGGAGGCCATCAAGATGGGGCAGTACAAGGAGAGC
TTCGCCAATGCCGGCTTCACCTCCTTTGACGTCGTGTCTCAGATGATGATGGAGGACATT
CTCCGGGTTGGGGTCACTTTGGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTG
ATGCGGGCGCAGATGAACCAGATTCAGTCTGTGGAGGTTTGACATTCACCTGCCTCGGCT
CACCTCTTCCTCCAAGCCCCGCCCCCTCTGCCCCACGTGCCGGCCCTCCTGGTGCTCTAT
CCACTGCAGGGCCAGCCACTCGCCAGGAGGCCACGGGCCACGGGAAGAACCAAGCGGTGC
CAGCCACGAGACGTCACCAAGAAAACATGCAACTCAAACGACGGAAAAAAAAAGGGAATG
GGAAAAAAGAAAACAGATCCTGGGAGGGGGCGGGAAATACAAGGAATATTTTTTAAAGAG
GATTCTCATAAGGAAAGCAATGACTGTTCTTGCGGGGGATAAAAAAGGGCTTGGGAGATT
CATGCGATGTGTCCAATCGGAGACAAAAGCAGTTTCTCTCCAACTCCCTCTGGGAAGGTG
ACCTGGCCAGAGCCAAGAAACACTTTCAGAAAAACAAATGTGAAGGGGAGAGACAGGGGC
CGCCCTTGGCTCCTGTCCCTGCTGCTCCTCTAGGCCTCACTCAACAACCAAGCGCCTGGA
GGACGGGACAGATGGACAGACAGCCACCCTGAGAACCCCTCTGGGAAAATCTATTCCTGC
CACCACTGGGCAAACAGAAGAATTTTTCTGTCTTTGGAGAGTATTTTAGAAACTCCAATG
AAAGACACTGTTTCTCCTGTTGGCTCACAGGGCTGAAAGGGGCTTTTGTCCTCCTGGGTC
AGGGAGAACGCGGGGACCCCAGAAAGGTCAGCCTTCCTGAGGATGGGCAACCCCCAGGTC
TGCAGCTCCAGGTACATATCACGCGCACAGCCTGGCAGCCTGGCCCTCCTGGTGCCCACT
CCCGCCAGCCCCTGCCTCGAGGACTGATACTGCAGTGACTGCCGTCAGCTCCGACTGCCG
CTGAGAAGGGTTGATCCTGCATCTGGGTTTGTTTACAGCAATTCCTGGACTCGGGGGTAT
TTTGGTCACAGGGTGGTTTTGGTTTAGGGGGTTTGTTTGTTGGGTTGTTTTTTGTTTTTT
GGTTTTTTTTAATGACAATGAAGTGACACTTTGACATTTCCTACCTTTTGAGGACTTGAT
CCTTCTCCAGGAAGAAGGTGCTTTCTGCTTACTGACTTAGGCAATACACCAAGGGCGAGA
TTTTATATGCACATTTCTGGATTTTTTTATACGGTTTTCATTGACACTCTTCCCTCCTCC
CACCTGCCACCAGGCCTCACCAAAGCCCACTGCCATGGGGCCATCTGGGCCATTCAGAGA
CTGGAGTGAGATTTGGGTGTGGAGGGGGAGGCGCCAAGGTGGAGGAGCTTCCCACTCCAG
GACTGTTGATGAAAGGGACAGATTGAGGAGGAAGTGGGCTCTGAGGCTGCAGGGCTGGAA
GTCCTTGCCCACTTCCCACTCTCCTGCCCCAATCTATCTAGTACTTCCCAGGCAAATAGG
CCCCTTTGAGGCTCCTGAGTGCCCTCAGATGGTCAAAACCCAGTTTTCCCTCTGGGAGCC
TAAACCAGGCTGCATCGGAGGCCAGGACCCGGATCATTCACTGTGATACCCTGCCCTCCA
GAGGGTGCGCTCAGAGACACGGGCAAGCATGCCTCTTCCCTTCCCTGGAGAGAAAGTGTG
TGATTTCTCTCCCACCTCCTTCCCCCCACCAGACCTTTGCTGGGCCTAAAGGTCTTGGCC
ATGGGGACGCCCTCAGTCTAGGGATCTGGCCACAGACTCCCTCCTGTGAACCAACACAGA
CACCCAAGCAGAGCAATCAGTTAGTGAATTG
Protein sequence predicted based on nucleotide sequence
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQ VCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADF DSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLY CNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGA TNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPF SPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSE YNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEK LPLIIGSSAAGLVFLIAVVVIAIVCNRRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDP FTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGY TEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFT VIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPT YTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAI EQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSG INLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVT LAGHQKKILNSIQVMRAQMNQIQSVEV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
