Gene:
Gene Symbol:
BLK
Gene Name:
B lymphoid tyrosine kinase
Original Clone ID:
FLH231821.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618094
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1518nts
Open reading frame: 223 to 1740
GGGAGGCTCTGATCGCAGACCGGGGGTGCTGCCACCTCTGTCTGCTGCCGGCAGAAAGCC
ACAAGCCATGAAAACTGATTGAGATGAGAAGAATTCATCTGGGACTGGCTTTTGCTTTAG
GATGGTGTTGGAAGTTGCTCGTTGTCGCTAGGAGCCTGCTCCACTGTAAGGGTGTCGGGA
TCTGAAGAGCTATGGTGAAACACCACTGAAGCATTGCCAAGGATGGGGCTGGTAAGTAGC
AAAAAGCCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGCCCCCTG
AAGGTCAGCGCCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCAC
CTTACTCCTCCACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTG
TATGACTACACCGCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAG
GTCCTGAAGGGAACTGGAGACTGGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGC
TATGTGCCCAGCAACTTTGTGGCCCGAGTGGAGAGCCTGGAAATGGAAAGGTGGTTCTTT
AGATCACAGGGTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCTCCAATCAACAAGGCCGGC
TCCTTTCTTATCAGAGAGAGTGAAACCAACAAAGGTGCCTTCTCCCTGTCTGTGAAGGAT
GTCACCACCCAGGGGGAGCTGATCAAGCACTATAAGATCCGCTGCCTGGATGAAGGGGGC
TACTACATCTCCCCCCGGATCACCTTCCCCTCGCTCCAGGCCCTGGTGCAGCACTATTCT
AAGAAGGGGGATGGTCTATGCCAGAGGCTGACCCTGCCCTGTGTGCGCCCGGCCCCGCAG
AATCCCTGGGCCCAGGATGAATGGGAGATCCCCCGGCAGTCTCTCAGGCTGGTCAGGAAA
CTCGGGTCTGGACAATTCGGCGAAGTCTGGATGGGTTACTACAAAAACAACATGAAGGTG
GCCATTAAGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCCTTCCTGGGTGAGGCCAAC
ATGATGAAGGCTCTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCACCAAGGAG
CCCATCTACATTGTCACCGAGTACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACA
GATGAAGGGAGCAGATTGTCACTCCCAAGGCTGATTGACATGTCGGCGCAGATTGCTGAA
GGGATGGCATACATTGAGCGCATGAATTCCATCCACCGCGACCTGCGGGCGGCCAACATC
CTGGTGTCTGAGGCCTTGTGCTGCAAAATTGCTGATTTTGGCTTGGCTCGAATCATCGAC
AGTGAATACACGGCCCAAGAGGGGGCCAAGTTCCCCATCAAGTGGACAGCCCCGGAAGCC
ATCCACTTCGGGGTCTTCACCATCAAAGCAGACGTGTGGTCGTTTGGAGTCCTCCTGATG
GAAGTTGTCACTTATGGGCGGGTGCCATACCCAGGGATGAGCAACCCCGAGGTCATCCGC
AACCTGGAGCGCGGCTACCGCATGCCGCGCCCCGACACCTGCCCGCCCGAGCTGTACCGC
GGCGTCATCGCCGAGTGCTGGCGCAGCCGGCCCGAGGAGCGGCCCACCTTCGAGTTCCTG
CAGTCGGTGCTGGAGGACTTCTACACGGCCACCGAGCGGCAGTACGAGCTGCAGCCCTAG
CCGGCCGCGCCCGCCTGCGCCCCGTGCCCACCTCTGCGCGGACGACCCCGACTTCCGTGC
CATCCCAGACGGGCCGCGAAGGCGGGGTGTCGCCTGTGCCCTTTTCTCAGACCCGGAATC
CAGTGGGCAGAGGCAGCTTCGCAGGGGGTCCCCGGACGGACTCCTTCACCGACTGCACCC
CCGGGCGAGTTACGCGGCCTCTCTGTGCCGCTTCATTTGTAGAGGGCTGTAACAGTGACC
TCGCACGGTCATCCGGAGTACTAAGCCCCAGTAAGGTGTTCAGGACTGGTAAGCGACTGT
CATCAAGTAAGGCCCCCGTGCTGGGCACCCCCCGTGCTGGCCGCGTCCCCGCCTCTGCGC
CCTGCGTGGACCCCGCCCTGCCCCGCTACAGAAGCCAGACTGGGTCCCGCGGACGCCAGC
AGGGGCAACCCCAGCCTAGGCTGCGCTCCAGCACTGCGGGGCTTTTCTGCAATAAAGTCA
CGAGCGTTCGAAAAA
Protein sequence predicted based on nucleotide sequence
MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDK HFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLE MERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIR CLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQS LRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANMMKALQHERLVRLY AVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRD LRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWS FGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEER PTFEFLQSVLEDFYTATERQYELQP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
