Gene:
Gene Symbol:
ACVR1
Gene Name:
activin A receptor, type I
Original Clone ID:
HsCD00299129
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618096
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1530nts
Open reading frame: 341 to 1870
GAAGCGAATAGCGTTTTCAGAGATATTGGGCGGCTCAAGGGTCTTACTCTGTCGCCCAGT
CTGTAATGCAGTGCTGTGACCATAGCCCACTGCAGCCTCCACCTCCCAGGCTCAAGCAGT
CCTTCCCCCCTCGCCCTCATGAATAGCTGGGACTACAGCCTGGAGCATTGGTAAGCGTCA
CACTGCCAAAGTGAGAGCTGCTGGAGAACTCATAATCCCAGGAACGCCTCTTCTACTCTC
CGAGTACCCCAGTGACCAGAGTGAGAGAAGCTCTGAACGAGGGCACGCGGCTTGAAGGAC
TGTGGGCAGATGTGACCAAGAGCCTGCATTAAGTTGTACAATGGTAGATGGAGTGATGAT
TCTTCCTGTGCTTATCATGATTGCTCTCCCCTCCCCTAGTATGGAAGATGAGAAGCCCAA
GGTCAACCCCAAACTCTACATGTGTGTGTGTGAAGGTCTCTCCTGCGGTAATGAGGACCA
CTGTGAAGGCCAGCAGTGCTTTTCCTCACTGAGCATCAACGATGGCTTCCACGTCTACCA
GAAAGGCTGCTTCCAGGTTTATGAGCAGGGAAAGATGACCTGTAAGACCCCGCCGTCCCC
TGGCCAAGCTGTGGAGTGCTGCCAAGGGGACTGGTGTAACAGGAACATCACGGCCCAGCT
GCCCACTAAAGGAAAATCCTTCCCTGGAACACAGAATTTCCACTTGGAGGTTGGCCTCAT
TATTCTCTCTGTAGTGTTCGCAGTATGTCTTTTAGCCTGCCTGCTGGGAGTTGCTCTCCG
AAAATTTAAAAGGCGCAACCAAGAACGCCTCAATCCCCGAGACGTGGAGTATGGCACTAT
CGAAGGGCTCATCACCACCAATGTTGGAGACAGCACTTTAGCAGATTTATTGGATCATTC
GTGTACATCAGGAAGTGGCTCTGGTCTTCCTTTTCTGGTACAAAGAACAGTGGCTCGCCA
GATTACACTGTTGGAGTGTGTCGGGAAAGGCAGGTATGGTGAGGTGTGGAGGGGCAGCTG
GCAAGGGGAAAATGTTGCCGTGAAGATCTTCTCCTCCCGTGATGAGAAGTCATGGTTCAG
GGAAACGGAATTGTACAACACTGTGATGCTGAGGCATGAAAATATCTTAGGTTTCATTGC
TTCAGACATGACATCAAGACACTCCAGTACCCAGCTGTGGTTAATTACACATTATCATGA
AATGGGATCGTTGTACGACTATCTTCAGCTTACTACTCTGGATACAGTTAGCTGCCTTCG
AATAGTGCTGTCCATAGCTAGTGGTCTTGCACATTTGCACATAGAGATATTTGGGACCCA
AGGGAAACCAGCCATTGCCCATCGAGATTTAAAGAGCAAAAATATTCTGGTTAAGAAGAA
TGGACAGTGTTGCATAGCAGATTTGGGCCTGGCAGTCATGCATTCCCAGAGCACCAATCA
GCTTGATGTGGGGAACAATCCCCGTGTGGGCACCAAGCGCTACATGGCCCCCGAAGTTCT
AGATGAAACCATCCAGGTGGATTGTTTCGATTCTTATAAAAGGGTCGATATTTGGGCCTT
TGGACTTGTTTTGTGGGAAGTGGCCAGGCGGATGGTGAGCAATGGTATAGTGGAGGATTA
CAAGCCACCGTTCTACGATGTGGTTCCCAATGACCCAAGTTTTGAAGATATGAGGAAGGT
AGTCTGTGTGGATCAACAAAGGCCAAACATACCCAACAGATGGTTCTCAGACCCGACATT
AACCTCTCTGGCCAAGCTAATGAAAGAATGCTGGTATCAAAATCCATCCGCAAGACTCAC
AGCACTGCGTATCAAAAAGACTTTGACCAAAATTGATAATTCCCTCGACAAATTGAAAAC
TGACTGTTGACATTTTCATAGTGTCAAGAAGGAAGATTTGACGTTGTTGTCATTGTCCAG
CTGGGACCTAATGCTGGCCTGACTGGTTGTCAGAATGGAATCCATCTGTCTCCCTCCCCA
AATGGCTGCTTTGACAAGGCAGACGTCGTACCCAGCCATGTGTTGGGGAGACATCAAAAC
CACCCTAACCTCGCTCGATGACTGTGAACTGGGCATTTCACGAACTGTTCACACTGCAGA
GACTAATGTTGGACAGACACTGTTGCAAAGGTAGGGACTGGAGGAACACAGAGAAATCCT
AAAAGAGATCTGGGCATTAAGTCAGTGGCTTTGCATAGCTTTCACAAGTCTCCTAGACAC
TCCCCACGGGAAACTCAAGGAGGTGGTGAATTTTTAATCAGCAATATTGCCTGTGCTTCT
CTTCTTTATTGCACTAGGAATTCTTTGCATTCCTTACTTGCACTGTTACTCTTAATTTTA
AAGACCCAACTTGCCAAAATGTTGGCTGCGTACTCCACTGGTCTGTCTTTGGATAATAGG
AATTCAATTTGGCAAAACAAAATGTAATGTCAGACTTTGCTGCATTTTACACATGTGCTG
ATGTTTACAATGATGCCGAACATTAGGAATTGTTTATACACAACTTTGCAAATTATTTAT
TACTTGTGCACTTAGTAGTTTTTACAAAACTGCTTTGTGCATATGTTAAAGCTTATTTTT
ATGTGGTCTTATGATTTTATTACAGAAATGTTTTTAACACTATACTCTAAAATGGACATT
TTCTTTTATTATCAGTTAAAATCACATTTTAAGTGCTTCACATTTGTATGTGTGTAGACT
GTAACTTTTTTTCAGTTCATATGCAGAACGTATTTAGCCATTACCCACGTGACACCACCG
AATATATTATCGATTTAGAAGCAAAGATTTCAGTAGAATTTTAGTCCTGAACGCTACGGG
GAAAATGCATTTTCTTCAGAATTATCCATTACGTGCATTTAAACTCTGCCAGAAAAAAAT
AACTATTTTGTTTTAATCTACTTTTTGTATTTAGTAGTTATTTGTATAAATTAAATAAAC
TGTTTTCAAGTC
Protein sequence predicted based on nucleotide sequence
MVDGVMILPVLIMIALPSPSMEDEKPKVNPKLYMCVCEGLSCGNEDHCEGQQCFSSLSIN DGFHVYQKGCFQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNF HLEVGLIILSVVFAVCLLACLLGVALRKFKRRNQERLNPRDVEYGTIEGLITTNVGDSTL ADLLDHSCTSGSGSGLPFLVQRTVARQITLLECVGKGRYGEVWRGSWQGENVAVKIFSSR DEKSWFRETELYNTVMLRHENILGFIASDMTSRHSSTQLWLITHYHEMGSLYDYLQLTTL DTVSCLRIVLSIASGLAHLHIEIFGTQGKPAIAHRDLKSKNILVKKNGQCCIADLGLAVM HSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSYKRVDIWAFGLVLWEVARRMVS NGIVEDYKPPFYDVVPNDPSFEDMRKVVCVDQQRPNIPNRWFSDPTLTSLAKLMKECWYQ NPSARLTALRIKKTLTKIDNSLDKLKTDC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
