metF (P. aeruginosa ) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  metF
Gene Name:  5,10-methylenetetrahydrofolate reductase
Original Clone ID:
FLH041784.01F
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
PaCD00006182
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 873nts
Open reading frame: 1 to 873

ATGGTCGCGTCCAAGGAACCGATCATGAGTCAGAGCGAACGCCGTTTCAGCTTCGAGTTC
TTCCCGGCGAAGACCGAGGCCGGCCATGAAAAGCTGTTGGCCGCCGCCCGCAACCTGGCG
GGCTACAAGCCCGACTTCTTCTCCTGCACCTACGGCGCCGGCGGATCCACCCGCGACCGC
ACGTTGAGTACCGTGCTGCAACTGGACGGCGAGGTGAAGGTGCCGACCGCGCCGCACCTG
TCCTGTGTCGGCGACTCGAAAGCCGAGTTGCGCGAACTGCTCGGCCGCTACCGCGAGGCC
GGCATCCGCCGCATCGTCGCCCTGCGCGGCGACCTGCCGTCGGGCATGGGCATGGCCAGC
GGCGAACTGCGCTACGCCAACGAACTGGTGGACTTCATCCGCACCGAGACCGGCGACCAC
TTCCACATCGAGGTCGCCGCCTATCCGGAGGTCCACCCCCAGGCGCGCAGCTTCGAGGAT
GACCTGGCGAACTTCGTGCGCAAGGTGAAGGCCGGCGCCAGCAGCGCCATCACCCAGTAC
TTCTTCAACGCCGATGCCTATTTCTACTTCGTCGAGCGGGTCGCCAAGCTCGGCGTGGAC
ATCCCGGTGGTCCCCGGCATCATGCCGATCACCAACTACTCCAAGCTGGCGCGCTTCTCC
GACGCCTGCGGCGCCGAACTGCCGCGCTGGATCCGCAAGCAACTGGAAGCCTACGGCGAC
GACAGCCGCAGCATCCAGGCCTTCGGCGAGCAGGTCATCAGCGAGATGTGCGAACGCCTG
CTGGAGGGCGGCGCACCGGGACTGCATTTCTATACTTTGAACCAGGCCGATCCGAGCCTG
GCGATCTGGAAGAATCTCCAGCTGCCACGCGGA

Protein sequence predicted based on nucleotide sequence

MVASKEPIMSQSERRFSFEFFPAKTEAGHEKLLAAARNLAGYKPDFFSCTYGAGGSTRDR
TLSTVLQLDGEVKVPTAPHLSCVGDSKAELRELLGRYREAGIRRIVALRGDLPSGMGMAS
GELRYANELVDFIRTETGDHFHIEVAAYPEVHPQARSFEDDLANFVRKVKAGASSAITQY
FFNADAYFYFVERVAKLGVDIPVVPGIMPITNYSKLARFSDACGAELPRWIRKQLEAYGD
DSRSIQAFGEQVISEMCERLLEGGAPGLHFYTLNQADPSLAIWKNLQLPRG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 873
Mutations:
None
Discrepancy:
a103g,T35A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable