Gene:
Gene Symbol:
MARK4
Gene Name:
MAP/microtubule affinity-regulating kinase 4
Original Clone ID:
FLH231544.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618259
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2067nts
Open reading frame: 6 to 2072
AGAAGATGTCTTCGCGGACGGTGCTGGCCCCGGGCAACGATCGGAACTCGGACACGCATG
GCACCTTGGGCAGTGGCCGCTCCTCGGACAAAGGCCCGTCCTGGTCCAGCCGCTCACTGG
GTGCCCGTTGCCGGAACTCCATCGCCTCCTGTCCCGAGGAGCAGCCCCACGTGGGCAACT
ACCGCCTGCTGAGGACCATTGGGAAGGGCAACTTTGCCAAAGTCAAGCTGGCTCGGCACA
TCCTCACTGGTCGGGAGGTTGCCATCAAGATTATCGACAAAACCCAGCTGAATCCCAGCA
GCCTGCAGAAGCTGTTCCGAGAAGTCCGCATCATGAAGGGCCTAAACCACCCCAACATCG
TGAAGCTCTTTGAGGTGATTGAGACTGAGAAGACGCTGTACCTGGTGATGGAGTACGCAA
GTGCTGGAGAAGTGTTTGACTACCTCGTGTCGCATGGCCGCATGAAGGAGAAGGAAGCTC
GAGCCAAGTTCCGACAGATTGTTTCGGCTGTGCACTATTGTCACCAGAAAAATATTGTAC
ACAGGGACCTGAAGGCTGAGAACCTCTTGCTGGATGCCGAGGCCAACATCAAGATTGCTG
ACTTTGGCTTCAGCAACGAGTTCACGCTGGGATCGAAGCTGGACACGTTCTGCGGGAGCC
CCCCATATGCCGCCCCGGAGCTGTTTCAGGGCAAGAAGTACGACGGGCCGGAGGTGGACA
TCTGGAGCCTGGGAGTCATCCTGTACACCCTCGTCAGCGGCTCCCTGCCCTTCGACGGGC
ACAACCTCAAGGAGCTGCGGGAGCGAGTACTCAGAGGGAAGTACCGGGTCCCTTTCTACA
TGTCAACAGACTGTGAGAGCATCCTGCGGAGATTTTTGGTGCTGAACCCAGCTAAACGCT
GTACTCTCGAGCAAATCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGT
TGAAGCCATACACAGAGCCCGAGGAGGACTTCGGGGACACCAAGAGAATTGAGGTGATGG
TGGGTATGGGCTACACACGGGAAGAAATCAAAGAGTCCTTGACCAGCCAGAAGTACAACG
AAGTGACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGGGTGGGGACCGGGGCG
CCCCAGGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGAACAAGTT
CCAGCAAAGGCACCAGCCACAGCAAAGGGCAGCGGAGTTCCTCTTCCACCTACCACCGCC
AGCGCAGGCATAGCGATTTCTGTGGCCCATCCCCTGCACCCCTGCACCCCAAACGCAGCC
CGACGAGCACGGGGGAGGCGGAGCTGAAGGAGGAGCGGCTGCCAGGCCGGAAGGCGAGCT
GCAGCACCGCGGGGAGTGGGAGTCGAGGGCTGCCCCCCTCCAGCCCCATGGTCAGCAGCG
CCCACAACCCCAACAAGGCAGAGATCCCAGAGCGGCGGAAGGACAGCACGAGCACCCCCA
ACAACCTCCCTCCTAGCATGATGACCCGCAGAAACACCTACGTTTGCACAGAACGCCCGG
GGGCTGAGCGCCCGTCACTGTTGCCAAATGGGAAAGAAAACAGCTCAGGCACCCCACGGG
TGCCCCCTGCCTCCCCCTCCAGTCACAGCCTGGCACCCCCATCAGGGGAGCGGAGCCGCC
TGGCACGCGGTTCCACCATCCGCAGCACCTTCCATGGTGGCCAGGTCCGGGACCGGCGGG
CAGGGGGTGGGGGTGGTGGGGGTGTGCAGAATGGGCCCCCTGCCTCTCCCACACTGGCCC
ATGAGGCTGCACCCCTGCCCGCCGGGCGGCCCCGCCCCACCACCAACCTCTTCACCAAGC
TGACCTCCAAACTGACCCGAAGGGTTACCCTCGATCCCTCTAAACGGCAGAACTCTAACC
GCTGTGTTTCGGGCGCCTCTCTGCCCCAGGGATCCAAGATCAGGTCGCAGACGAACCTGA
GAGAATCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATCAAACGGAAACCGC
CCCCCGGCTGCTCCGATTCCCCTGGAGTGTGAAGCTGACCAGCTCGCGCCCTCCTGAGGC
CCTGATGGCAGCTCTGCGCCAGGCCACAGCAGCCGCCCGCTGCCGCTGCCGCCAGCCACA
GCCGTTCCTGCTGGCCTGCCTGCACGGGGGTGCGGGCGGGCCCGAGCCCCTGTCCCACTT
CGAAGTGGAGGTCTGCCAGCTGCCCCGGCCAGGCTTGCGGGGAGTTCTCTTCCGCCGTGT
GGCGGGCACCGCCCTGGCCTTCCGCACCCTCGTCACCCGCATCTCCAACGACCTCGAGCT
CTGAGCCACCACGGTCCCAGGGCCCTTACTCTTCCTCTCCCTTGTCGCCTTCACTTCTAC
AGGAGGGGAAGGGGCCAGGGAGGGGATTCTCCCTTTATCATCACCTCAGTTTCCCTGAAT
TATATTTGGGGGCAAAGATTGTCCCCTCTGCTGTTCTCTGGGGCCGCTCAGCACAGAAGA
AGGATGAGGGGGCTCAGCGGGGGGAGCTGGCACCTTCCTGGAGCCTCCAGCCAGTCCTGT
CCTCCCTCGCCCTACCAAGAGGGCACCTGAGGAGACTTTGGGGACAGGGCAGGGGCAGGG
AGGGAAACTGAGGAAATCTTCCATTCCTCCCAACAGCTCAAAATTAGGCCTTGGGCAGGG
GCAGGGAGAGCTGCTGAGCCTAAAGACTGGAGAATCTGGGGGACTGGGAGTGGGGGTCAG
AGAGGCAGATTCCTTCCCCTCCCGTCCCCTCACGCTCAAACCCCCACTTCCTGCCCCAGG
CTGGCGCGGGGCACTTTGTACAAATCCTTGTAAATACCCCACACCCTCCCCTCTGCAAAG
GTCTCTTGAGGAGCTGCCGCTGTCACCTACGGTTTTTAAGTTATTACACCCCGACCCTCC
TCCTGTCAGCCCCCTCACCTGCAGCCTGTTGCCCAATAAATTTAAGAGAGTCCCCCCCTC
CCCAATGCTGACCCTAGGATTTTCCTTCCCTGCCCTCACCTGCAAATGAGTTAAAGAAGA
GGCGTGGGAATCCAGGCAGTGGTTTTTCCTTTCGGAGCCTCGGTTTTCTCATCTGCAGAA
TGGGAGCGGTGGGGGTGGGAAGGTAAGGATGGTCGTGGAAGAAGGCAGGATGGAACTCGG
CCTCATCCCCGAGGCCCCAGTTCCTATATCGGGCCCCCCATTCATCCACTCACACTCCCA
GCCACCATGTTACACTGGACTCTAAGCCACTTCTTACTCCAGTAGTAAATTTATTCAATA
AACAATCATTGACCCATGCCTACTCCATGCCAGGCCCAGTGCTGGACACAGAGACATGAA
GCTCTGTCTGTGGGAGACAGGGATTCTGACACAGACACCGGACAAACCATTGTCTTGGGG
AGCCCAGAAGAGAAAGTGGGCAGGGTGGGGTCATTGGGGAAGATGCTCTAGAGGAATTAA
TGCTGGAATGGGGTGTTGAAGGATGAGTAGGAGTTAGTTAGGCATTGAGTTTGCCCTGGG
CAAAAGCCCAGAAGTGGGAGTATGTGGTATATCTTCAGAGAACTGGGTAATTTCAGTGTG
GCTGCTGTGTTGGGCATGGATGGAGAATCAGCAAGAGAAATGCTGTATTAGGACTAATAA
TCCATCTACGCTGCTTAAGCAAAAAGGTATTTGTTGGTTTATGTTACTTAATAGTCCAGG
GGCACCTGGCTTCAGGTAGGTTTGATCCAGGCATCAGGCCATTGCATCTATTTTTTCAGT
GTAAGTTGAATTCTAGTAATTTTTATCAAGTAAGGGCTCCTTTCCTGGTGGCACAGATGA
CTTCAGCAGTTAGAAGTTTCTATCCCTCCAGCTTTCTGCAGCAGAAAGACCCTCATTGTC
AGTTTCCCAGCAAAAGTCCCAGGGCAGACTCTCATTGGCCCAAATGGGCCATGTGATTTT
CTCTAAACCAATCACTGTGACTCTAGAGTGGCCAGACTCAGAGCTGCACTTAGTAGGGGT
TCCTCAAAGGAAGGTCAAGTGTCATGAGCAGGAGAAAAGGCATGGGAGCTGGACAGATTA
TAGTGGTTGAAGTCTGTGCAGTACAGAAGGGCGGAGCTTATTCACACAGCACCTTTGGGG
CCAAAATGAATAAGCTGGACTTTCTCCCCATGGCACTGGGGAACCATGGAAGTTCAGGGA
ACTTCAGGGAAGAGGCTTGGTCAATTCCTGAGAGCATCCTCTGTGCTGGGGACACAGTGG
TAATCAAGACAGCCCCAACACTGCCCTCATAGAGCTCACAGTCCAATGGAGGAGGCAGAT
GTGTCCTCAGGCAGCGACTGGGCAGGGCTGGTATAGGGGAGTCCAGAGGTGATGCCTGCC
TCAGCCAGGGAGGGCTTCCTGGAGGAGAAGGAGCCAGCTAGACATGGATAGGAGTGCGTT
TTAGGCACAGCAAATGGCACATACAAGGGCCAGGGAGCAAGAGAGAGGACAGGTCCTCAA
CAAATGGCATGTGACTTTGTAAGTGTAGAATTGCTGTGAGGTATGGGGCTAGGGGCGTCA
GTAGGGCCTTGAAGGTTATGGACAGGGGCCTGGGCTTTCTTCCAAGGGCACTGGGGGAGC
CATGGCAAGGTTGTAGGTAGGGTAGAGATGGGCGGGTTTGTGCTATGTGCAGGGTGGAAG
GGAGGGAAGTTGACAGGTCAGAAGATCAGGAAAGAGGTCGGGGCTGGACAGATGGGGAGA
GCGCAGATAGATTTAAGAGAGTCCTGTGAGGCAAAGTGGGCAGGACCTGGTAACAGGTGT
CTGGACTGTGGCTTTGGCTGGCTCAGAAGGTCCCCACTGGCGTGTGTGGTCTATGTAGCC
TCTGGGTGTGGAGCTGGGATCTTCAACTGGGGACAGTACAGTAAAGAACATCACAGC
Protein sequence predicted based on nucleotide sequence
MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYR LLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVK LFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHR DLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIW SLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCT LEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEV TATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQR RHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAH NPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVP PASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHE AAPLPAGRPRPTTNLFTKLTSKLTRRVTLDPSKRQNSNRCVSGASLPQGSKIRSQTNLRE SGDLRSQVAIYLGIKRKPPPGCSDSPGV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
