Gene:
Gene Symbol:
PKN3
Gene Name:
protein kinase N3
Original Clone ID:
HsCD00301070
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618262
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2670nts
Open reading frame: 16 to 2685
GGCCGGAGCGGCGCCATGGAGGAGGGGGCGCCGCGGCAGCCTGGGCCGAGCCAGTGGCCC
CCAGAGGATGAGAAGGAGGTGATCCGCCGGGCCATCCAGAAAGAGCTGAAGATCAAGGAG
GGGGTGGAGAACCTGCGGCGCGTGGCCACAGACCGCCGCCACTTGGGCCATGTGCAGCAG
CTGCTGCGGTCCTCCAACCGCCGCCTGGAGCAGCTGCATGGCGAGCTGCGGGAGCTGCAC
GCCCGAATCCTGCTGCCCGGCCCTGGGCCTGGCCCAGCTGAGCCTGTGGCCTCAGGACCC
CGGCCGTGGGCAGAGCAGCTCAGGGCTCGGCACCTAGAGGCTCTCCGGAGGCAGCTGCAT
GTGGAGCTGAAGGTGAAGCAGGGGGCTGAGAACATGACCCACACGTGCGCCAGTGGCACC
CCCAAGGAGAGGAAGCTCCTGGCAGCTGCCCAGCAGATGCTGCGGGACAGCCAGCTGAAG
GTGGCCCTGCTGCGGATGAAGATCAGCAGCCTGGAGGCCAGTGGGTCCCCGGAGCCAGGG
CCTGAGCTGCTGGCGGAGGAGCTACAGCATCGACTGCACGTTGAGGCAGCTGTGGCTGAG
GGCGCCAAGAACGTGGTGAAACTGCTTAGTAGCCGGAGAACACAGGACCGCAAGGCACTG
GCTGAGGCCCAGGCCCAGCTACAGGAGTCCTCTCAGAAACTGGACCTCCTGCGCCTGGCC
TTGGAGCAGCTGCTGGAGCAACTGCCTCCTGCCCACCCTTTGCGCAGCAGAGTGACCCGA
GAGTTGCGGGCTGCGGTGCCTGGATACCCCCAGCCTTCAGGGACACCTGTGAAGCCCACC
GCCCTAACAGGGACACTGCAGGTCCGCCTCCTGGGCTGTGAACAGTTGCTGACAGCCGTG
CCTGGGCGCTCCCCAGCGGCCGCACTGGCCAGCAGCCCCTCCGAGGGCTGGCTTCGGACC
AAGGCCAAGCACCAGCGTGGCCGAGGCGAGCTTGCCAGCGAGGTGCTGGCTGTGCTAAAG
GTGGACAACCGTGTTGTGGGGCAGACGGGCTGGGGGCAGGTGGCCGAACAGTCCTGGGAC
CAGACCTTTGTCATCCCACTGGAGCGAGCCCGTGAGCTGGAGATTGGGGTACACTGGCGG
GACTGGCGGCAGCTATGTGGCGTGGCCTTCCTGAGACTTGAGGACTTCCTGGACAATGCC
TGTCACCAACTGTCCCTCAGCCTGGTACCGCAGGGACTGCTTTTTGCCCAGGTGACCTTC
TGCGATCCTGTCATTGAGAGGCGGCCCCGGCTGCAGAGGCAGGAACGCATCTTCTCTAAA
CGCAGAGGCCAGGACTTCCTGAGGGCTTCGCAGATGAACCTCGGCATGGCGGCCTGGGGG
CGCCTCGTCATGAACCTGCTGCCCCCCTGCAGCTCCCCGAGCACAATCAGCCCCCCTAAA
GGATGCCCTCGGACCCCAACAACACTGCGAGAGGCCTCTGACCCTGCCACTCCCAGTAAT
TTCCTGCCCAAGAAGACCCCCTTGGGTGAAGAGATGACACCCCCACCCAAGCCCCCACGC
CTCTACCTCCCCCAGGAGCCAACATCCGAGGAGACTCCGCGCACCAAACGTCCCCATATG
GAGCCTAGGACTCGACGTGGGCCATCTCCACCAGCCTCCCCCACCAGGAAACCCCCTCGG
CTTCAGGACTTCCGCTGCTTAGCTGTGCTGGGCCGGGGACACTTTGGGAAGGTCCTCCTG
GTCCAGTTCAAGGGGACAGGGAAATACTACGCCATCAAAGCACTGAAGAAGCAGGAGGTG
CTCAGCCGGGACGAGATAGAGAGCCTGTACTGCGAGAAGCGGATCCTGGAGGCTGTGGGC
TGCACAGGGCACCCTTTCCTGCTCTCCCTCCTTGCCTGCTTCCAGACCTCCAGCCATGCC
TGCTTTGTGACTGAGTTTGTGCCTGGTGGTGACCTCATGATGCAGATCCACGAGGATGTC
TTCCCCGAGCCCCAGGCCCGCTTCTACGTGGCTTGTGTTGTCCTGGGGCTGCAGTTCTTA
CACGAGAAGAAGATCATTTACAGGGACCTGAAGTTGGATAACCTTCTGCTGGATGCCCAG
GGATTCCTGAAGATCGCAGACTTTGGACTCTGCAAGGAAGGGATCGGCTTCGGGGACCGG
ACTAGCACCTTCTGTGGCACCCCGGAGTTCCTGGCTCCCGAGGTGCTGACCCAGGAGGCA
TACACACGGGCTGTGGACTGGTGGGGGCTGGGTGTGCTGCTCTACGAGATGCTGGTGGGT
GAGTGCCCGTTCCCAGGGGACACAGAGGAAGAGGTGTTTGACTGCATCGTCAACATGGAC
GCCCCCTACCCCGGCTTTCTGTCGGTGCAAGGGCTTGAGTTCATTCAGAAGCTCCTCCAG
AAGTGCCCGGAGAAGCGCCTCGGGGCAGGTGAGCAGGATGCCGAGGAGATCAAGGTCCAG
CCATTCTTCAGGACCACCAACTGGCAAGCCCTGCTCGCCCGCACCATCCAGCCCCCCTTC
GTGCCTACCCTGTGTGGCCCTGCGGACCTGCGCTACTTTGAGGGCGAGTTCACAGGGCTG
CCGCCTGCCCTGACCCCACCTGCACCCCACAGCCTCCTCACTGCCCGCCAACAGGCCGCC
TTCCGGGACTTCGACTTTGTGTCAGAGCGATTCCTGGAACCCTGAGGGCATCTCCTGGCA
CCTCTGTCCCCTTCCCCCACAGACTGTTAGAGCCTCTGCTCGTTCACCCGTGCGCCCTGC
CTGGAGGTCCAGGCCTTGCTGGGTACTTCTGAGCCCTTGGGATTCAAAGTGGCAGCCATG
GGGCCACTGTTGTGGGCTTTGCTCAGTGTCACTGGGCAAAGTGTGTCCCTTCCCCCTCCA
GCTCGCCCTCTTCTACCTCCCAGCGAGACCTGGCCCAGAAAGGGTGCCGCAGCAAGGAGT
GATATGGTTTGTCTTTTTAAGACTGGACTTGCTTTATATTAAATTTGTAAAAAAAAAAAA
AAAAAA
Protein sequence predicted based on nucleotide sequence
MEEGAPRQPGPSQWPPEDEKEVIRRAIQKELKIKEGVENLRRVATDRRHLGHVQQLLRSS NRRLEQLHGELRELHARILLPGPGPGPAEPVASGPRPWAEQLRARHLEALRRQLHVELKV KQGAENMTHTCASGTPKERKLLAAAQQMLRDSQLKVALLRMKISSLEASGSPEPGPELLA EELQHRLHVEAAVAEGAKNVVKLLSSRRTQDRKALAEAQAQLQESSQKLDLLRLALEQLL EQLPPAHPLRSRVTRELRAAVPGYPQPSGTPVKPTALTGTLQVRLLGCEQLLTAVPGRSP AAALASSPSEGWLRTKAKHQRGRGELASEVLAVLKVDNRVVGQTGWGQVAEQSWDQTFVI PLERARELEIGVHWRDWRQLCGVAFLRLEDFLDNACHQLSLSLVPQGLLFAQVTFCDPVI ERRPRLQRQERIFSKRRGQDFLRASQMNLGMAAWGRLVMNLLPPCSSPSTISPPKGCPRT PTTLREASDPATPSNFLPKKTPLGEEMTPPPKPPRLYLPQEPTSEETPRTKRPHMEPRTR RGPSPPASPTRKPPRLQDFRCLAVLGRGHFGKVLLVQFKGTGKYYAIKALKKQEVLSRDE IESLYCEKRILEAVGCTGHPFLLSLLACFQTSSHACFVTEFVPGGDLMMQIHEDVFPEPQ ARFYVACVVLGLQFLHEKKIIYRDLKLDNLLLDAQGFLKIADFGLCKEGIGFGDRTSTFC GTPEFLAPEVLTQEAYTRAVDWWGLGVLLYEMLVGECPFPGDTEEEVFDCIVNMDAPYPG FLSVQGLEFIQKLLQKCPEKRLGAGEQDAEEIKVQPFFRTTNWQALLARTIQPPFVPTLC GPADLRYFEGEFTGLPPALTPPAPHSLLTARQQAAFRDFDFVSERFLEP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
