Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pJFT7_nGST_DC-r2
Source:
Center for Personalized Diagnostics
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
None
Publications:
None
Authors:
Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
EvNO00623103
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pJFT7_nGST_DC-r2
Synonyms:
''
Sequencing Primer:
Forward:
GSTseqF
Reverse:
pCPD6268_R
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6012
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3297
3979
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2572
2781
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
GSTseqF forward sequencing primer GCAAGTATATAGCATGGCCT
1105
1124
primer
reverse
pCPD6268_R reverse sequencing primer agccaactcagcttccttt
2993
3011
promoter
T7
T7 promoter
5997
6012
protease cleavage site
TEV
TEV protease cleavage site
1209
1229
recombination site
attR1
attR1 recombination site
1245
1261
recombination site
attR2
attR1 recombination site
2932
2947
reporter gene
LacZ
LacZ alpha
5833
5901
selectable marker
AmpR
Ampicillin resistance
4407
4736
selectable marker
CmR
chloramphenicol resistance (removed in plasmids containing a gene insert)
1478
2134
ssDNA origin
f1
f1 origin
5505
5811
tag
GST
N-terminal GST tag
525
1190
trxn termination sequence
T7 term
T7 terminator
3069
3115
