metK (P. aeruginosa ) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  metK
Gene Name:  S-adenosylmethionine synthetase
Original Clone ID:
FLH038472.01F
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
PaCD00006236
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1191nts
Open reading frame: 1 to 1191

ATGAGCGAATATTCCGTTTTCACCTCCGAGTCCGTGTCCGAAGGCCATCCGGACAAGATC
GCGGACCAGATCTCCGACGCGGTGCTGGACGCCATCATCGCCAAGGACAAGTACGCTCGC
GTGGCATGCGAAACCCTGGTCAAGACCGGCGTGGCGATCATCGCCGGTGAAGTCACCACC
TCCGCCTGGGTCGACCTGGAAGAGCTGGTGCGCAAGGTCATCATCGACATCGGCTACGAC
AGCTCCGACGTCGGCTTCGATGGTGCCACCTGCGGCGTGCTGAACATCATCGGCAAGCAG
TCGGTGGACATCAACCAGGGCGTCGACCGGGCCAAGCCGGAAGACCAGGGCGCCGGCGAC
CAGGGCCTGATGTTCGGCTACGCCAGCAACGAGACCGACGTGCTGATGCCGGCGCCGATC
TGTTTCTCCCACCGCCTGGTCGAGCGCCAGGCCGAGGCGCGCAAGTCCGGCCTGCTGCCA
TGGCTGCGCCCGGACGCCAAGTCCCAGGTGACCTGCCGCTACGAAGGCGGCAAGGTGGTC
GGCATCGATGCGGTGGTGCTGTCCACCCAGCACAACCCGGAAGTCTCCTATAACGACCTG
CGCGACGGCGTGATGGAGCTGATCATCAAGCAGGTGCTGCCGGCCGAGCTGCTGCACAAG
GACACCCAGTTCCACATCAACCCGGCCGGCAACTTCGTGATCGGCGGCCCGGTCGGCGAC
TGCGGCCTGACCGGACGCAAGATCATCGTCGACTCCTACGGCGGCATGGCCCGCCACGGC
GGCGGCGCGTTCTCCGGCAGGGACCCGTCCAAGGTCGACCGCTCCGCCGCCTACGCCGGC
CGCTACGTGGCGAAGAACATCGTCGCCGCCGGCCTCGCCGAGCGTTGCGAGATCCAGGTT
TCCTACGCCATCGGCGTGGCCCAGCCGACCTCGATCTCGATCAACACCTTCGGCACCGGC
AAGGTCAGCGACGAGAAGATCGTGCAACTGGTCCGCGAGCACTTCGACCTGCGCCCGTAC
GCGATCACCAAGATGCTCGACCTGCTCCACCCGATGTACCAGCCGACCGCGGCCTACGGC
CACTTCGGTCGTCATCCGTTCGAGCTGACCGTGGACGGCGATACCTTCACCGCGTTCACC
TGGGAGAAGACCGACAAGGCCGCTCTCCTGCGCGACGCCGCCGGCCTCGGA

Protein sequence predicted based on nucleotide sequence

MSEYSVFTSESVSEGHPDKIADQISDAVLDAIIAKDKYARVACETLVKTGVAIIAGEVTT
SAWVDLEELVRKVIIDIGYDSSDVGFDGATCGVLNIIGKQSVDINQGVDRAKPEDQGAGD
QGLMFGYASNETDVLMPAPICFSHRLVERQAEARKSGLLPWLRPDAKSQVTCRYEGGKVV
GIDAVVLSTQHNPEVSYNDLRDGVMELIIKQVLPAELLHKDTQFHINPAGNFVIGGPVGD
CGLTGRKIIVDSYGGMARHGGGAFSGRDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
SYAIGVAQPTSISINTFGTGKVSDEKIVQLVREHFDLRPYAITKMLDLLHPMYQPTAAYG
HFGRHPFELTVDGDTFTAFTWEKTDKAALLRDAAGLG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1191
Mutations:
None
Discrepancy:
a685g,T229A; a800g,K267R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable