Slc12a3 (Mus musculus) in pPICZa

Explanation of Terms

Gene:
Gene Symbol:  Slc12a3
Gene Name:  solute carrier family 12, member 3
Original Clone ID:
TPDB-0108
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pPICZa
Format: 
FUSION
Source:
Center for the X-ray Structure Determination of Human Transporters
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Center for the X-ray Structure Determination of Human Transporters
MmCD00627268
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 3000nts
Open reading frame: 1 to 3000

GCAGAGCTCCCCGTGACAGAGCTGCCTGGCGATGCTCTGTGCAGTGGGCGCTTCACCATC
AGCACACTGATGGGAGGCGATGAGCCCCCACCAGCTGCCTGTGACAGCAGCCAGCCCAGC
CACTTAACACACGGCAGCACCTTATACATGCGCACCTTCGGTTACAACACCATAGACGTG
GTGCCGGCCTACGAACACTATGCTAACAGCGCTCTGCCCGGTGAGCCCCGGAAGGTGCGG
CCCACCCTGGCTGACCTGCATTCATTCCTCAAGGAAGGTAGCCACCTGCATGCCCTGGCC
TTTGATGGACGGCAAGGTCGTGAGCTGACTGATGGACTTGTGGAAGATGAGACAGGCACC
AACAGTGAGAAGAGCCCTGGAGAACCTGTTCGCTTCGGCTGGGTCAAGGGGGTGATGATC
CGTTGCATGCTCAACATCTGGGGAGTGATCCTCTACCTGCGACTCCCCTGGATTACGGCG
CAGGCGGGCATCGTGCTGACTTGGCTCATCATCCTGCTGTCGGTCATGGTGACATCCATC
ACCGGCCTCTCCATCTCCGCCATCTCTACCAATGGCAAGGTCAAGTCGGGTGGCACCTAT
TTCCTTATTTCCCGGAGTCTGGGACCAGAGCTGGGAGGCTCCATTGGCCTCATCTTCGCC
TTTGCCAATGCTGTGGGTGTGGCCATGCACACTGTGGGCTTTGCAGAAACCGTAAGGGAC
CTACTGCAGGAGTATGGCACACCCATCGTAGACCCCATCAATGACATCCGCATCATCGGT
GTGGTCACGGTCACTGTGCTGCTGGCCATCTCGCTGGCTGGGATGGAGTGGGAGTCCAAG
GCCCAGGTGCTATTCTTTCTTGTCATCATGGTCTCCTTTGCCAACTACCTGGTGGGCACA
CTGATCCCAGCATCTGAAGACAAAGCCTCTAAAGGCTTCTACAGCTACCATGGGGACATT
TTTGTTCAGAACCTGGTACCCGACTGGAGAGGCATTGATGGTAGCTTCTTTGGCATGTTC
TCCATCTTCTTCCCTTCGGCCACTGGCATTCTGGCAGGGGCCAACATCTCCGGGGACCTC
AAGGATCCTGCTGTAGCCATCCCCAAGGGAACACTCATGGCCATTTTCTGGACCACCATC
TCCTACCTTGCCATCTCAGCTACCATCGGCTCCTGTGTGGTGAGAGATGCCTCTGGGGAT
GTGAACGACACCATGACTCCTGGTCCAGGCCCCTGCGAAGGGCTAGCCTGTGGCTATGGC
TGGAACTTCACAGAATGCTCCCAGCAGCGCAGTTGCCGCTATGGCCTCATCAACTACTAC
CAGACCATGAGCATGGTGTCTGCCTTCGCACCCCTGATCACAGCCGGCATCTTTGGCGCC
ACCCTGTCTTCTGCCTTGGCCTGTCTTGTCTCGGCTGCTAAGGTCTTCCAGTGCCTGTGT
GAAGACCAGCTGTACCCTCTGATTGGCTTTTTTGGCAAAGGCTATGGCAAGAACAGGGAG
CCTGTGCGTGGCTACCTGCTGGCCTATGCCATTGCTGTGGCCTTCATCATCATTGCTGAA
CTTAACACCATCGCCCCCATCATTTCCAACTTCTTCCTCTGCTCCTACGCCCTCATCAAT
TTCAGCTGCTTCCATGCCTCCATCACCAACTCACCTGGATGGAGACCCTCCTTCCGATAC
TACAGCAAGTGGGCGGCGCTGTTTGGGGCAGTGATCTCGGTGGTCATCATGTTCCTGCTT
ACCTGGTGGGCTGCTCTCATTGCCATCGGAGTCGTCCTCTTCCTCCTGCTCTATGTGATC
TACAAGAAGCCAGAGGTGAACTGGGGTTCCTCAGTACAGGCTGGCTCCTACAATCTGGCC
CTGAGCTACTCCGTGGGCCTCAACGAGGTGGAAGACCACATCAAGAACTACCGCCCCCAG
TGCCTGGTGCTTACAGGGCCCCCCAACTTCCGCCCAGCCCTTGTGGACTTTGTGAGCACA
TTTACCCAGAACCTAAGCCTAATGATTTGTGGTCATGTGCTCATTGGACCTGGCAAGCAG
AGAGTGCCCGAGCTCCGGCTCATCGCCAGTGGACACACCAAGTGGCTGAACAAGAGGAAG
ATCAAGGCCTTCTACTCTGACGTCATCGCTGAGGACTTGCGCAGTGGCGTCCAGATTCTC
ATGCAGGCCTCAGGGCTTGGGAGAATGAAGCCGAACATTCTGGTGGTTGGATTCAAGAGG
AACTGGCAGTCTGCCCACCCAGCCACAGTGGAGGACTACATTGGTGTCCTGCATGATGCC
TTTGACTTCAACTACGGGGTTTGTGTCATGAGGATGCGCGAGGGGCTCAATGTCTCCGAG
GCTCTGCAGACCCACACAACCCCAGAGGCATTGATACAAGAGGAGCAGGCTAGCACCATC
TTCCAGTCGGAGCAAGGCAAGAAGACCATTGACATCTACTGGCTGTTCGATGATGGAGGT
CTCACCCTCCTCATCCCCTATCTCCTGCACCGCAAGAAGAGATGGGGCAAATGCAAGATC
CGAGTGTTTGTTGGGGGCCAGATCAACAGGATGGACGAAGAGAGAAAGGCGATCATCTCT
CTGTTGAGCAAGTTCAGACTGGGATTCCATGAAGTTCACGTCCTTCCCGACATCAACCAG
AAGCCCCAGGCCGAGCACACCAAGCGGTTTGAGGACATGATTGCACCCTTCCGCCTCAAT
GATGGCTTCAAGGACGAGGCCACCGTCACTGAGATGAGGAGGGACTGTCCCTGGAAGATC
TCAGATGAAGAGATTAACAAGAACAGAATTAAGTCCCTTCGGCAGGTGAGACTGAGTGAG
ATACTGCTGGATTACTCTCGGGATGCTGCTCTGATCATCCTTACCCTGCCCATTGGAAGG
AAGGGGAAGTGCCCCAGTTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTC
AGACCTCCCGTCCTCCTCATCCGAGGCAACCAGGAGAATGTGCTCACCTTTTACTGCCAG

Protein sequence predicted based on nucleotide sequence

AELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDV
VPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDGRQGRELTDGLVEDETGT
NSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSI
TGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRD
LLQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGT
LIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDL
KDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYG
WNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLC
EDQLYPLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALIN
FSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVI
YKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVST
FTQNLSLMICGHVLIGPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQIL
MQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVCVMRMREGLNVSE
ALQTHTTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKI
RVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAEHTKRFEDMIAPFRLN
DGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGR
KGKCPSSLYMAWLETLSQDLRPPVLLIRGNQENVLTFYCQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  Pichia    Marker:  zeocin
Host Type:  bacterial    Marker:  zeocin
Bacterial Selection Condition: 50 ug/ml zeocin
Growth Condition: Growth in low salt LB with 50ug/ml zeocin at 37 degrees C
Comments: None
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinPurified: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in TPDB-0108
Recommended expression in:
Not Applicable