Gene:
Gene Symbol:
PIGN
Gene Name:
phosphatidylinositol glycan anchor biosynthesis, class N
Original Clone ID:
TPDB-0084
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pPICZa
Format:
FUSION
Source:
Center for the X-ray Structure Determination of Human Transporters
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Center for the X-ray Structure Determination of Human Transporters
Center for the X-ray Structure Determination of Human Transporters
HsCD00627282
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2790nts
Open reading frame: 1 to 2790
CTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTTGAC
ATTTATTTTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCTCCT
CCAGCGAGAAGATTAGTGTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTACGAA
TTAGATGAAAATGGAAACTCTAGAGCACCGTTTATTAGGAATATCATAATGCATGAAGGC
AGCTGGGGCATATCTCATACACGTGTGCCAACAGAATCTCGGCCAGGTCATGTAGCTCTG
ATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGATGGAAGGAAAATCCTGTA
GAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGCCCAGATATC
CTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGATGCT
AAAAGAGAGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGATAAT
GTTAAGGACTTCTTTCATCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAATGAA
GAGAAAATAGTTTTTTTCTTACATTTATTAGGAATAGATACAAACGGACATGCTCATCGA
CCATCCTCGAGAGACTACAAGGACAATATTAAAAAAGTTGATGATGGAGTTAAAGAAATC
GTGTCTATGTTTAACCATTTCTATGGAAATGATGGGAAAACAACATTTATCTTTACCTCT
GACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCTTCAGAGACTTTAACT
CCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAGCAATTT
GATGATGCATTTTTGAAAGAGTGGAGATTGGAGAATTGGAAGAGGCTAGATGTCAATCAG
GCTGATATTGCACCATTGATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCAGTG
GGAATCCTTCCTGTGGATTATCTTAACAACACTGATCTCTTCAAAGCAGAGAGCATGTTT
ACAAATGCAGTACAGATTCTTGAACAGTTCAAGGTGAAAATGACTCAGAAGAAAGAAGTT
ACTTTACCATTTTTGTTTACACCATTTAAACTGCTTTCTGATTCCAAACAGTTCAACATT
TTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTTGATGAAGTGGTCTCCCTTTGC
AAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACATATGACAGATTC
TTTTTGGGCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCTTTGTTG
ATCATCAAGTCTCATTCCAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCAAGC
CATCTCCTGCCTTGTAGTTTTGTAGCTATTGGCATTTTAGTAGCATTTTTTCTGCTGATT
CAAGCCTGTCCCTGGACATATTATGTATATGGTTTGTTGCCACTGCCAATATGGTATGCG
GTTCTAAGAGAATTTCAAGTTATTCAGGACCTTGTTGTATCAGTGTTGACCTATCCTCTG
AGCCATTTTGTTGGGTACCTGTTAGCCTTTACCCTGGGAATTGAAGTATTAGTTCTCAGT
TTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTTGCAGCTTGGCCATTTCTC
ACTCGGCTGTGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTCTCTTTGCTC
CTGGCAGTGTTCCCACTGATGCCGGTTGTAGGTCGAAAGCCAGACATCTCTCTAGTGATG
GGTGCAGGCTTGCTGGTTCTTCTGTTATCCCTGTGTGTTGTAACATCTCTCATGAAAAGA
AAAGATAGCTTTATAAAGGAAGAGCTATTGGTACATCTGTTACAGGTGCTGAGCACAGTG
CTCTCCATGTATGTTGTGTATAGCACTCAGAGTAGTCTACTCAGGAAGCAAGGACTGCCT
CTCATGAATCAAATTATTAGCTGGGCAACATTAGCCTCTTCCTTGGTTGTGCCACTACTG
AGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATACTTCTTTCATTGATGTCAACCTAC
CTACTTCTAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTGTCTTGTTTGATGTTT
GTCTGGATAAACATAGAACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAACAAAAG
CTCACCAGTATCCAGTTCTCTTATAATACTGATATAACTCAGTTTCGACAGCTATATCTG
GATGACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCTTAGTGACAGCATTTTTTGGAACT
GGAAATATAGCTTCTATTAACAGCTTTGATCTTGCCTCTGTCTATTGCTTTCTGACTGTG
TTCAGTCCTTTTATGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTTCTT
GTTATGTGTGCTTTTGAAGCAGTTCAGTTGACTACTCAGTTATCGTCAAAAAGCCTTTTT
CTCATTGTTCTCGTCATATCAGACATTATGGCTTTGCATTTTTTCTTCTTGGTCAAGGAT
TATGGCAGCTGGCTTGATATTGGGACAAGCATCAGCCACTATGTGATTGTCATGTCCATG
ACCATCTTTTTGGTGTTCCTCAATGGCCTGGCCCAGCTGCTCACAACGAAGAAACTCAGA
CTATGTGGCAAACCCAAAAGTCACTTCATG
Protein sequence predicted based on nucleotide sequence
LLFFTLGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPARRLVLFVADGLRADALYE LDENGNSRAPFIRNIIMHEGSWGISHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPV EFDSLFNESKYTWSWGSPDILPMFAKGASGDHVYTYSYDAKREDFGAQDATKLDTWVFDN VKDFFHHARNNQSLFSKINEEKIVFFLHLLGIDTNGHAHRPSSRDYKDNIKKVDDGVKEI VSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETLTPLVTWGAGIKYPQRVSAQQF DDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVGILPVDYLNNTDLFKAESMF TNAVQILEQFKVKMTQKKEVTLPFLFTPFKLLSDSKQFNILRKARSYIKHRKFDEVVSLC KELIHLALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLIIKSHSNLIKGVSKEVKKPS HLLPCSFVAIGILVAFFLLIQACPWTYYVYGLLPLPIWYAVLREFQVIQDLVVSVLTYPL SHFVGYLLAFTLGIEVLVLSFFYRYMLTAGLTAFAAWPFLTRLWTRAKMTSLSWTFFSLL LAVFPLMPVVGRKPDISLVMGAGLLVLLLSLCVVTSLMKRKDSFIKEELLVHLLQVLSTV LSMYVVYSTQSSLLRKQGLPLMNQIISWATLASSLVVPLLSSPVLFQRLFSILLSLMSTY LLLSTGYEALFPLVLSCLMFVWINIEQETLQQSGVCCKQKLTSIQFSYNTDITQFRQLYL DDIRRAFFLVFFLVTAFFGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFVL VMCAFEAVQLTTQLSSKSLFLIVLVISDIMALHFFFLVKDYGSWLDIGTSISHYVIVMSM TIFLVFLNGLAQLLTTKKLRLCGKPKSHFM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
Pichia
Marker:
zeocin
Host Type: bacterial Marker: zeocin
Host Type: bacterial Marker: zeocin
Bacterial Selection Condition:
50 ug/ml zeocin
Growth Condition:
Growth in low salt LB with 50ug/ml zeocin at 37 degrees C
Comments:
None
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinPurified: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in TPDB-0084
Recommended expression in:
Not Applicable
Vector Name:
pPICZa
Synonyms:
''
Sequencing Primer:
Forward:
AOX1
Reverse:
pAOX1 Reverse
Description:
with tag/fusion/marker assay, multiple cloning site clone, expression in Pichia pastoris, yeast expression expression with AOX1 and MCS and c-myc and His and TEF1 and EM7 and CYC1 and pUC ori and ZeoR; zeocin resistance in bacterial, zeocin resistance in Pichia;
Comments:
None
Size (bp):
3329
Parent Vector:
None
Properties:
expression in Pichia pastoris, multiple cloning site, with tag/fusion/marker, yeast expression
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
932
1011
bacterial origin
pUC ori
pUC origin
2602
3275
promoter
AOX1
AOX1 promoter
1
941
promoter
TEF1
fragment containing TEF1 promoter
1419
1830
promoter
EM7
EM7 promoter
1831
1898
selectable marker
ZeoR
Zeocin resistance
1899
2273
tag
c-myc
N-terminal C-myc tag
1012
1044
tag
His
C-terminal 6xHis tag
1057
1077
trxn termination sequence
CYC1
CYC1 transcription termination region
2274
2591
