Gene:
Gene Symbol:
MANEA
Gene Name:
mannosidase, endo-alpha
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00630559
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1397nts
Open reading frame: 112 to 1500
GGTCGGCTCTATGTTCGCGGTCTTAACCTCTCCTCTGGCCGAGTCCTTGCAAGAAGTGAA
TTACCCGACCCTGCAAAACACTTACTATTTTGGAGTTTAAAGTATGTCATCATGGCAAAG
TTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTCTCTCTGATG
ATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTTGGACTTGAC
CTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGATTTCCAAAAG
AGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAAAAAGTGTTGAAATCACTATG
AAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTACCACCTCTGAACAATTATCTA
CATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGATGGTAAATATATACATTGG
AATCATCCAGTGTTAGGGCATTGGGACCCTAGAATAGCCAAGAATTATCCACAAGGGAGA
CACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGTTACAGTTCT
CGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCAATTGGTGTA
CTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCTACTGATAAC
TTGGTACCCACTATTTTGGATAAAGCTCATAAATATAACCTAAAGGTTACTTTTCACATA
GAACCATATAGCAATCGAGATGATCAAAACATGTACAAAAATGTCAAGTATATTATAGAC
AAATATGGAAATCATCCGGCCTTTTACAGGTACAAGACGAAGACTGGCAATGCTCTTCCT
ATGTTTTATGTCTATGATTCCTATATTACCAAGCCTGAAAAATGGGCCAATCTGTTAACC
ACCTCAGGGTCTCGGAGTATTCGCAATTCTCCTTATGATGGACTGTTTATTGCCCTTCTG
GTAGAAGAAAAACATAAGTATGATATTCTTCAAAGTGGTTTTGATGGAATTTACACATAT
TTTGCCACAAATGGCTTTACTTATGGCTCATCACATCAGAATTGGGCTAGCCTAAAATTA
TTTTGTGATAAATACAACTTAATATTTATCCCAAGTGTGGGCCCAGGATACATAGATACC
AGCATCCGTCCATGGAACACGCAAAACACTCGGAACCGAATCAATGGGAAGTATTATGAA
ATTGGTCTGAGTGCCGCACTTCAGACACGCCCCAGCTTAATTTCTATCACCTCTTTTAAT
GAGTGGCATGAAGGAACTCAGATTGAAAAAGCTGTTCCCAAAAGAACCAGTAATACAGTG
TACCTAGATTACCGTCCTCATAAACCAGGTCTTTACCTAGAACTGACTCGCAAGTGGTCT
GAAAAATACAGTAAGGAAAGAGCAACTTATGCATTAGATCGCCAGCTGCCTGTTTCTTAA
TGCATTGATTAAAGTTTAATAGTTATCAAAATCACCTAATTTTTAAAAATAGCTTTCGTT
TTGAGTTCTGGAAAGAAAACTGTCAAAATCAGTATATACTATTAGTTATATTTAAAAATA
TTTTTTTAAATTCTTTACAGATAATATTATACTTGTTACCCTTCACAATACCACATGAGA
AAATATCTGAGACAAAATGTATACAAATATATTCCTTATGGCATAATTTATTGCATTTCT
GACTGAAATCAAAATTCTGATTTGATGGCAATTGAATTTTCATTTTACAATAGATAAATG
CTTGTGTTACCTAAAGCACTTAGCACACAGTTAAATTATATTTACATCCTAGACCCAAAT
AAATAGGATTGTGTGTATATTTGGGATATCTATTGAAGAAAAAAAGAAAACCCCTTAAAG
ATAATGTACATGCTTCATGTCATGTCTTTAAAATAATTTAATCAACTTTATTGTCTTAGT
ATTTAGACTCTGGATAACTCTACAATAATGAGGAAATTCTTAAGAATAACAAAATCACTG
TACCTTCCTCTCAATTTTGCTGTGAACCTGAAATGGCTTTAAATTAATACTCTTATTTTT
TATTTAATTTAATTACATAAATTAAACCTTACCATGACCAAATTGTGTTAGGAAGGCCTG
CTATCTACAGCACAGTGTGTCATTTGCAGATTTGTGGTTACCTATACCACGCTAGGTGTT
TTGACATGTTTAGTGTTTCTGCTTTACAGTGCTGAATTCCATATTTTAGAAGCTATGAAA
GTCCTTTTATGAAAAAGTTACTGATTGCTTCTCAGTTATTAGGAAAACAGTTGTTTCACA
ATTATTATGTAGATATGATGCCCAAATATCATTTTTAGTATATCTTGTCGATCTTTAAGT
TGTTACTATTGTGTTATTCATGTCTTTAAATCAGATACCAAATATTTTTTAGGAAAGAAA
AATGTTATTACTGTCATTAGGTTGTCTTTTAATACTTTAAGTTATTTTGACGAAAAGTAA
TAGAGAAAATTTACTTAGCATTTTAGATTCTAGAGACATGGAAATGAAAATTATTTTATG
TCTAGAGTAGGTCCTGAAGTTTGGCTTTACATTAAGTTTAGCACTGTATCAGAATGAAGA
AACTAATATTTTACATAAAAACTAATACTTTCAATTTTTTATATAGTAATATCCCCATTT
TGTAAATGTTAGACTTTTATCATACCTGTAAGTTAAAATACTTGTTATCAATAACTTGTC
ATATTGTGACAAATTGATCACTTGTGTACGAAAAATAAATCTCCTTAAAAACTAAATAAA
ATGCACTGTATTCTTACAGTTAATGTTTATAACTATAGTAAAAAATTAATATATATCCTA
TTACATAAATGTTATTTCTTAGGTGTTCCATTAAGAAGAGCAATAGAATAATGCTAAAAA
ATAATGCCTATAAATCTTCAGAGTATAAAGACATCCATTCAGAAACAAAAATTAGCACTA
AATTTTTTATAAAATAGACCAGATGACAAAATTTATTTTATTTTTAAACAGTGGTTTTGA
CACAAATTATGTTATTGAAAAGCATTATTAATGTTTAATTTATTTAAAATTTTGGAATTT
GCCATTTCTCAGACAATGATCAGGCCTTAGGAAATTAATACAGTAGTAGTAATCATTTTC
TAGGGGAAAATAAAAGAATAAATCACTATACTGATATTTTGATATAAGCAAGCACTTACA
TGGTAATCACTATATAGATCCAACCTGTGGATTTTCTTCTTATGTCCATTTAACTAGAAT
ATATTATTTTAGGTATAATTTACAAATGTCACACCTAATAATCTTTTATAATATACCATA
TTTCATTAAAGTTTTGTTAGAGAAGTATCTACCACAGAGGAGTTTTTGTCATTGTGTACG
TTGTGTATTTGAACCCACCATGACAGAAAGTAAATTTTAGGAAATAGTTATGAGATTAAG
GGAAAATCTATAAAAACAAGGTTAGCATATTCTCAACACAGATACCACCACTTTCTTTTT
CCCATTATAGACATGGTGAATCCACACAGCATACTTCATCTCTGAGCTTTGTTGTGATTC
CTCAACACATTACCCTAACCAGCCAGCAGTAACAGATTTCAGAGTAAGATAAAGCAGATT
CTGTCTTCATTGCAAAAAGTTATTCTCAATGGAAGAATGGCATCTGATCTCATAATTACT
AGTTTATATTAATATAGTTTTTTTCTCCCTTTTTAATAAAATAATTACAGTCATCCCTCA
GTGTCTGTGGGGGATTGGTTCCAGTTACCCCTATAGATACCAAAATCTGCAGATGCTCAA
GTCCCTGATATAAACTGGCATAGTAGTTGCATATAATCTATGCACATCCTCCTGTATACA
TTAAGTCATCTCTAGATTATTTATAACACTTAATACAATGTAAATGCTATGTAGTTGTTA
TACCATACTGGTTAGTGAATAATTACATGAAAAAAAAGAGTCTGTACATCTTCAGAGTTT
CAGTCGGCAATTTCTTGGCCATGGATGTAGAACCTACAGATAAGGTGAGCCAACTGCATT
AGGAAATAACTCTAATAATTCTGTTAATTCTTAGAGAGGAAAACTTTCAAAATCTTCCTC
AGGTATTTATTACAACTGCCTTTACCATTTTAGTTGTAACACAGTTTAAATTGTTATGAT
AACAAGTAAATAAGAGCAAAGAATTTATTTCTTAATTCAAAACTATACGTTTGAATTCAA
TATGGTATAACTTAAAGTGGTATAATACATACAATGCATGAATCATAATGGATTCTTTTA
TAAGTTATTAATTTTTATGGTTTAATCAGTCTAATTGTTTTGACTGTTATAGAAACCAAA
TATTTTACTGTTTCTTTTAAGGACTAATATTGTCAAAAACTGCTGTTATTAACTTCACTT
GAGTTGTTTAACTTCCTTCTGTTTTAAGATTGTAATTAAAAATTACTATTTTGTTATATG
GAATGGTTAATTTTTACCTAATAAAAACATAGATGAAATAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MAKFRRRTCIILALFILFIFSLMMGLKMLRPNTATFGAPFGLDLLPELHQRTIHLGKNFD FQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNYLHVFYYSWYGNPQFDGKY IHWNHPVLGHWDPRIAKNYPQGRHNPPDDIGSSFYPELGSYSSRDPSVIETHMRQMRSAS IGVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLKVTFHIEPYSNRDDQNMYKNVKY IIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPEKWANLLTTSGSRSIRNSPYDGLFI ALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSSHQNWASLKLFCDKYNLIFIPSVGPGY IDTSIRPWNTQNTRNRINGKYYEIGLSAALQTRPSLISITSFNEWHEGTQIEKAVPKRTS NTVYLDYRPHKPGLYLELTRKWSEKYSKERATYALDRQLPVS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
