Gene:
Gene Symbol:
NFAT5
Gene Name:
nuclear factor of activated T-cells 5, tonicity-responsive
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00630636
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4647nts
Open reading frame: 1 to 4647
ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCC
CTCTACTCGCGAGATTCTCTGAAGTTACACCCATCACAGAATTTTCATAGAGCTGGACTA
TTGGAAGAATCTGTCTATGATCTTCTCCCAAAGGAGTTACAGTTACCTCCATCTAGAGAA
ACATCTGTAGCATCAATGAGTCAGACAAGCGGTGGTGAGACAGGCTCGCCTCCTCCAGCT
GTTGTTGCTGCTGATGCTTCTTCAGCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGC
TCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACAGCAAGGCT
ATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAG
CAGTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCGAAGAGGCACACAGTCTTG
TACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAG
GGGTGTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAAC
TTCAGTAACATGAGCACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGA
AAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGATTGTGAAGAATCTAATATG
GATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGCTTACCACG
GACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAAAACCAAAAAGGAACTGGA
GTAAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAAAGTGAGGGAAAGGAGCTG
AAGATAGTTGTACAACCTGAGACACAGCACCGAGCTCGGTACCTGACTGAGGGCAGCCGT
GGCTCAGTGAAAGATAGAACACAGCAAGGCTTTCCTACAGTAAAGCTGGAAGGCCATAAT
GAACCTGTAGTGTTGCAAGTGTTTGTGGGCAACGACTCTGGACGAGTGAAACCACATGGA
TTTTATCAGGCCTGCAGAGTAACTGGACGAAATACAACTCCTTGCAAAGAAGTGGACATT
GAAGGCACTACTGTTATAGAAGTCGGCCTTGATCCTAGCAACAACATGACACTGGCGGTG
GACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAAGCCAGAATAGGAATTGCT
GGTTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCATGAGGAAA
GATGGCTCCACTTTGACACTGCAAACACCCTCTTCTCCAATTTTGTGTACTCAGCCAGCA
GGAGTGCCAGAAATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAAGAAGAA
GTGTTTTTAATCGGCAAGAACTTTCTGAAAGGAACTAAAGTTATTTTCCAAGAAAATGTT
TCTGATGAAAACTCTTGGAAGTCAGAAGCTGAAATTGATATGGAACTATTTCATCAGAAT
CATCTTATTGTGAAGGTTCCTCCCTATCATGACCAACATATAACTTTGCCTGTGTCAGTG
GGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTTCAACCATTCACTTACACT
CCAGACCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAGAAGGAAATATCTAGTCCAGCA
AGACCTTGCTCTTTTGAAGAGGCCATGAAAGCAATGAAAACTACTGGATGTAATTTAGAT
AAGGTAAATATTATCCCTAATGCCCTGATGACTCCACTCATACCAAGCAGTATGATTAAG
AGTGAAGATGTTACTCCAATGGAAGTAACAGCAGAAAAAAGATCTTCCACTATTTTTAAG
ACTACAAAGTCTGTTGGATCAACTCAGCAAACATTAGAAAACATCTCAAACATAGCAGGA
AATGGCTCTTTTTCATCACCATCATCTTCCCACCTACCTTCTGAAAATGAAAAACAGCAG
CAGATTCAGCCCAAGGCATACAACCCAGAGACCCTGACAACTATTCAAACCCAGGACATC
TCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGTCAGCTGCCCAACAGCGATGCA
CTATTGCAGCAGGCTACACAGTTTCAGACAAGAGAAACTCAGTCTAGAGAGATATTACAG
TCAGATGGTACAGTGGTTAATTTGTCACAACTGACTGAGGCATCACAACAACAGCAGCAG
TCACCACTACAAGAACAAGCACAGACTTTACAGCAGCAGATTTCATCAAATATTTTTCCA
TCACCAAATAGTGTGAGTCAGCTTCAGAATACTATTCAGCAGCTGCAAGCAGGGAGTTTC
ACAGGCAGTACTGCTAGTGGCAGCAGTGGAAGTGTTGACTTGGTCCAACAAGTTTTAGAG
GCACAGCAGCAGTTATCTTCAGTTTTATTTTCTGCTCCAGATGGTAATGAGAATGTTCAA
GAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGTCAAATTCAGAGTGGTGTAAGCCCT
GGAATGTTTTCCTCAACAGAGCCAACAGTCCATACCAGACCAGATAATTTATTACCTGGA
AGAGCTGAAAGTGTTCATCCACAGTCTGAAAACACGTTATCTAATCAACAGCAGCAGCAG
CAGCAGCAACAGCAAGTGATGGAATCTTCAGCCGCAATGGTGATGGAGATGCAACAGAGT
ATCTGCCAGGCAGCTGCCCAGATTCAGTCAGAGTTATTCCCTTCAACTGCTTCAGCAAAT
GGAAACCTTCAGCAATCGCCAGTTTACCAGCAGACTTCTCACATGATGAGTGCATTGTCT
ACCAATGAGGATATGCAAATGCAGTGTGAATTGTTTTCTTCTCCTCCTGCAGTTTCTGGA
AATGAAACTTCTACAACTACCACACAGCAGGTTGCAACCCCTGGCACTACCATGTTTCAG
ACATCAAGTTCAGGAGATGGAGAAGAAACTGGAACACAAGCAAAACAGATTCAGAACAGT
GTCTTTCAGACCATGGTCCAAATGCAACATAGTGGGGACAATCAACCTCAAGTTAACCTT
TTTTCATCCACAAAAAGTATGATGAGTGTTCAGAATAGTGGTACCCAACAACAAGGTAAT
GGTTTATTCCAGCAAGGGAATGAGATGATGTCACTTCAATCTGGAAATTTTTTGCAGCAG
TCTTCTCATTCACAGGCCCAACTTTTTCATCCTCAAAATCCTATTGCCGATGCTCAGAAC
CTTTCCCAGGAAACTCAAGGTTCTCTCTTTCATAGTCCAAATCCTATTGTCCACAGTCAG
ACTTCTACAACCTCCTCTGAACAAATGCAGCCTCCAATGTTTCACTCTCAAAGTACCATT
GCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCAACCAACATATTTCTTTCC
CAGAGTCCCATGAATAATCTTCAGACTAACACAGTAGCCCAAGAAGCATTTTTTGCAGCA
CCGAACTCAATTTCTCCACTTCAGTCAACATCAAACAGTGAACAACAAGCTGCTTTCCAA
CAGCAAGCTCCAATATCACACATCCAGACTCCTATGCTTTCCCAAGAACAGGCACAACCC
CCGCAGCAGGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCACCTAATCCAATG
CCTCAAAGCCAACAAGGAACCATGTTCCAGTCACAGCACTCAATAGTTGCCATGCAGAGT
AACTCTCCATCCCAGGAACAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAA
CAACAACAGAGCATTTTATTCAGTAATCAGAATACCATGGCTACAATGGCGTCTCCAAAG
CAACCACCACCAAACATGATATTCAACCCAAATCAAAATCCAATGGCTAATCAGGAGCAA
CAGAACCAGTCAATTTTTCACCAACAAAGTAACATGGCCCCAATGAATCAAGAGCAACAG
CCCATGCAATTTCAGAGTCAGTCCACAGTTTCCTCACTTCAGAACCCAGGTCCTACCCAG
TCGGAATCATCACAGACCCCCTTGTTCCATAGCTCTCCTCAGATTCAGTTGGTACAAGGG
TCACCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTATCTCCAGCATCCATGTCTGCC
TTGCAGACCAGTATAAATCAACAAGATATGCAACAGTCTCCTCTTTATTCCCCTCAGAAC
AACATGCCTGGAATTCAAGGAGCCACATCTTCGCCTCAACCACAGGCTACTTTATTTCAC
AACACAGCAGGAGGCACAATGAACCAACTGCAGAATTCTCCTGGCTCATCTCAGCAGACA
TCAGGAATGTTCTTATTTGGCATTCAAAATAACTGTAGTCAGCTTTTAACCTCTGGACCA
GCTACATTGCCTGATCAGTTGATGGCCATAAGTCAGCCAGGCCAACCACAAAACGAGGGC
CAGCCACCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAGAATTCTCCACTGGCATCC
TCTATAAACACCAACCAGAACATCGAAAAGATTGATTTGCTTGTTTCATTGCAAAACCAA
GGGAACAACTTGACTGGCTCCTTTTTG
Protein sequence predicted based on nucleotide sequence
MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRE TSVASMSQTSGGETGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKA MQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDE GCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNM DIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKEL KIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHG FYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIA GSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEE VFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSV GIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLD KVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAG NGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDA LLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFP SPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQ EQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQ QQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALS TNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNS VFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQ SSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTI AVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQ QQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQS NSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQ QNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQG SPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFH NTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEG QPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSFL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
