rne (F. tularensis subsp. tularensis SCHU S4 )
In pDEST17 (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH230349.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDEST17
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
FtCD00063113
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2658nts
Open reading frame: 1 to 2658

ATGAAAAGAATACTAATAAATAGCAAGAGTGGCGAAGAAACAAGAATTGCCACCCTAGAT
AATGGCAAATTAATAGATTTAGACATTGAGAGCGTTGATAGAGAACAAAAAAAAGCAAAT
ATTTACAAAGGTTATATTTCAAGAATTGAACCAAGTTTAAATGCTATATTTGTTAACTAT
GGTGAAGAAAAAAATGGTTTTTTACCATTTAAAGAAGTATCAGAGTATTATCTAAAAGAT
GTTCCAAACGGTGAAAATATCGCACCTTTGCTTTCAGAGGGGCAAGAACTAATTGTTCAA
ATAGATAAAGAAGAACGAGGCGATAAAGGTGCAGCATTAACTACATTTATTACCTTGGCT
GGTTCGTATATGGTGTTATTGCCAAATAATCCTGAGGGGGGTGGTATTTCCCGTCGTGTC
GAAGGTGAAGATAGAGAAAGATTAAAAAAATATCTCAAAGAATTAAATATCCCAAAAAAT
ATGAGCGTAATAGCAAGAACTGCTTGCGTTGAGTGCTCTTTTGAAGAATTAAAACATGAT
TTTGATACTCTAGTTGAATTATGGGGATCTATTTCACAAGCTTACCATAGAATCAAAAAA
CCAGCTTTATTACATAAAGAAAGTGACATTATTGTAAGGACAGTTAGAGATCACTTAAAA
GAAGATGTTAAAGAAATCATCGTTGATTCTAAAGAGTGCTTTGAAGATGTCAAAAGACAG
CTTAGTTTACTTAGACAAAGTTTTGATATAAACAAAGTAAAACTATACAACGAAGAATTA
CCATTATTTGCTCAGTTTGGTATTGATCAACAAATAGAAAATGCTTATAAAAGAGAAATT
CGCCTACCATCCGGCGGTTCTATCGTCATAGATACTACAGAGGCACTTGTAGCAATAGAT
GTAAACTCATCTCGCGCTAACAAAGCCGAAGATGTAGAAACTACAGCATTTAAAACTAAC
CTAGAGGCTGCGGAAGAAGTTGCTCGTCAACTTAGAATTAGAGACTTAGGTGGTCTTGTT
ATAGTTGACTTTATTGATATGTCTTTCTACCCGAATCGAAAACAAGTCGAAGAAAAACTA
ATGGAAGCACTACAACAAGATAGAGCACGCATCCAAATGTCACGCATTTCTAAACTTGGA
CTTGTTGAGATCTCAAGACAACGTTTAAGTTCTTCAATTAATGAAAGCGTAATGCAAAAA
TGCCCACGTTGTGAGGGTCATGGTTTTATCAAAACTACGCAAGCTACAGCACTTACCATC
CTACGTAAAATCAGAGCAGAAGCCATTAAAGAAGATACCAACGAAATTCGTGTCCAAGTT
CCTGTAGACATCGCAGCATATATACTAAATGAAAAAAGAGATAGTATTGTTGAGATTGAG
AGAATTTCTCAAGTTAAGGTTATGATTATTCCTAACTTTAATATGGAATCGCCAAAGTTT
CAAATGCAGAGAATCTGGGGTACTAGCTACAAATCAAATCGTACAAGCTCAGAATTAATA
GAAGATGTTTATAATATTGAAATTCCTAAAGCTGGTAAGAAAAAAATTGCGGCTGTGGAT
TTAAATCAAGCTATTGAAAATCAAGCTGCTAGCAACACTCAAGAACAAAAGCCTCAAACT
GATGCTCAAGTAGATATAGTTACACAAAATGATAAGAAACAAGATGATGTAACTCAACAA
GCTAAGAAAAAAGGCTTCTTTGCTCGGCTTTTTAGCGGTATATTTGCAACTGATTCAAAA
GCTGCTCAGCAAGCTAATAACTCAGCTCAAAAACAACAAAACAAAGAACAGAATTTCCAA
AGGCAGCAACAACATAATAAGGCGCAAAAAGATAATAATCAACGTAATGAGCGTAACAAT
AGCAACAACAAAAATGAACGCAATAAAAATAGAAACAATAAAGGAGCAAAACCACAAATA
GCTCAAGAAAATAAACCACAAAAAGACAATAGCCAGCGTAGTGATCTCAATGCTAATGGT
AACAGTAACAATGATAAAAATGAGCGCAATAAAAATAAATCAAACTATGATAAGTTTGAT
AAAAAATCAAATAACCGTAACAGCAGAAGCAATAATTTAAATCTAAATAATGCTGAAGAA
GTTATTGATATTACGCAACTTAAGTATCAAAATCAAACAGCTATTGCAAAGACAGATAAT
ATCAAAAAGGTGATTTCGAAGAATCCTGATGAGTTTATCTCTGTAATGGTTAAAGATGTC
TTAGAAAACTATGATAGTCTAAAAGACGATGGTGCTACAAAGATAGCTACCCAGGAGAAA
ATTAAAACTAACAAATACTTAAAGTTTGATACTGTATCTGTAGATACTGAAATAGCTCTA
CTTAATGAAGTCGCTAAATCAAATCAAACTGTTGGAGTTGAGCAAATCACAGCTGAAACA
GAAGAATCTTTAAGTATCGTTGATAAAGTTGATGACATAACAATACCAGAAGTTGTAGTA
GAGCAAGAAAAGCCAGCGAAAGTAGTTAAAGAAACTAATCAAACTAGTGAAGCTAAACCT
AAAAAAGAAAAGCAAACTACTACAAAAAATAATAAGAAAGAAAAACCAGCTGAAAAAGAG
ATGAAACAAAATCAAGCTAAACCTGAATATATAAACTATTCACCAGCGATTGATTTTGAA
TCTCAAGCACTAATATAG

Protein sequence predicted based on nucleotide sequence

MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNY
GEEKNGFLPFKEVSEYYLKDVPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLA
GSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKNMSVIARTACVECSFEELKHD
FDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ
LSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAID
VNSSRANKAEDVETTAFKTNLEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKL
MEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQKCPRCEGHGFIKTTQATALTI
LRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF
QMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQT
DAQVDIVTQNDKKQDDVTQQAKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQ
RQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQIAQENKPQKDNSQRSDLNANG
NSNNDKNERNKNKSNYDKFDKKSNNRNSRSNNLNLNNAEEVIDITQLKYQNQTAIAKTDN
IKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIAL
LNEVAKSNQTVGVEQITAETEESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKP
KKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFESQALI*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2658
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable