Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pCMVi-LS
Source:
Center for Membrane Proteins of Infectious Diseases
Description:
with tag/fusion/marker assay, multiple cloning site clone, bacterial expression expression with leader sequence and MCS and intron and hGH and forward primer and AmpR and ColE and f1 and reverse primer and DTH and -10/Spec-R and Stop Codons and CMV and SV40; ampicillin resistance in bacterial;
Comments:
None
Publications:
Authors:
Arizona State University
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Center for Innovations in Medicine
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Center for Innovations in Medicine
EvNO00631283
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pCMVi-LS
Synonyms:
''
Description:
with tag/fusion/marker assay, multiple cloning site clone, bacterial expression expression with leader sequence and MCS and intron and hGH and forward primer and AmpR and ColE and f1 and reverse primer and DTH and -10/Spec-R and Stop Codons and CMV and SV40; ampicillin resistance in bacterial;
Comments:
Clone insert in-frame using the BglII restriction sites and another downstream site before the transcription terminator (such as XmaI)
Size (bp):
5067
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Arizona State University
PSI:Biology
Center for Innovations in Medicine
PSI:Biology
Center for Innovations in Medicine
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
-10 signal
-10/Spec-R
0
0
3' Clip
DTH
1024
1893
MCS
MCS
Multiple cloning site; clone insert in-frame using multiple cloning site with BglII, KpnI, MluI, ClaI, SalI, XbaI, BamHI, SmaI
1155
1233
Miscellaneous Feature
Stop Codons
1219
1224
Origin
SV40
1910
2217
bacterial origin
ColE
ColE1 origin
2574
3430
intron
intron
857
989
leader sequence
leader sequence
N-terminal "LS" = secretion leader sequence from human alpha1-antitrypsin
1081
1152
primer
forward primer
LSFOR forward sequencing primer TTCTGTCTCGTGGGGCAT
1089
1106
primer
reverse primer
HGHTER reverse sequencing primer ACTGGAGTGGCAACTTCCAGG
1272
1292
promoter
CMV
CMV promoter
1
744
selectable marker
AmpR
ampicillin resistance
3440
4350
ssDNA origin
f1
f1 origin
4332
4873
trxn termination sequence
hGH
hGH terminator
1234
1710
