Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH233131.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDEST17
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
FtCD00063249
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2742nts
Open reading frame: 1 to 2742
ATGATTCAAGATTCTATACAATTTCTGACTAATTCTAGAAAATCAAAAATATTAGATGAA
ATTATTAATCAGTTTGAGAATTGTGAAGAGTTTATCATAAGTGTTGCCTTTATTACTTTT
AGTGGAGTTGTATGTATCTTAGAGGTATTACGTCAACTAAGAGCTAAAGGGATAAAGGGT
AAAATTCTTACAGGCTGTTATTTAAATTTTACCGAACCAAAAGCGCTTAAGAAACTCCTT
GAGTTTGATAATATTGAGTTAAAAATCCTTAATGATGAAAACTTTCATGCTAAGGGATTC
TTTTTTCGTAATAAAGATAGTTGGCAGATAATTCTAGGTAGCTCAAATCTAACTCAATCG
GCTCTTACAATTAATAATGAGTGGAATTTGCTTATTAAGACAAGTTTGGCTGATGTTACA
ACACAGCAAATTCTTGCAGAGTTTGATTATCTTTTTGATAGGGCGAAATATCCTAGCGAT
ATCTTAGATGAGTATATTCAAATATATCAGCGTGCTAGTAAGTTAGCTAATACAAGTATT
CAACGTATAAGTCATAAAATACAGCCGAATAAAATTCAATGTGAAGCACTTGAAAGTTTA
AGGCAGTTAAGAGATAGTGGCAAAAACAAGGCTTTAATAATAAGTGCTACTGGTACGGGC
AAGACTTTTCTAAGTGCCTTTGATATTGCAAGTATAAAACCAAAAAGATGTTTATTTGTC
GTACATCGTACAAATATTGCTATAAAAGCACAAGAGACTTTTGCTAAAGTTATTAGCGAT
AAAACCTTAGGTCTATATACAGGTGGTAATAAAACTTCAGCAGAGTATTTATTTGCTACA
GTACAGACACTTAAAAACTCAAAAGTTTTAGCTAATTTTAAAAGCCAAGAATTTGAATAC
ATAATCATCGACGAAGTTCATCATGCAGAAGCTGAAAGTTACAAAAAGGTTTTAGCACAC
TTTACGCCAAAATTTTTGCTTGGAATGACAGCCACTCCAGAACGTAGTGATGCTGCCGAT
ATATTCAAACTATTTGATTACAATATAGCTTATGAAATTAGACTCCATCAAGCCCTAGAG
CAAGATTTGTTATGTCCATTTCATTATTTTGCTGTTGAAGATTTGTATCTTGAGTCCGAA
GAGATTTTAGCCAAAAATTTTGCCAAACTTGTTAGTGATGTGCGTGTTGAGCATATCATC
GAAAAGATCAATTTTTATGGTTATAGTGGTCAATCTAGAGCGGCACTAATGTTTGTCAGT
AATGTAGATGAGGCAAAAGAGCTTGCTACTAAATTTAGTAACAAAGGTATAAAGGCTAAA
GCTTTATCAAGTCAAGATTCTGAGCAAATACGCCAAACAACTATAGCACAGCTTGAGAGC
GGTGAACTTGAGATTATTGTGACAGTTGATATCTTTAATGAAGGGGTTGATATCCCATGT
ATCAATCAGATTATCCTTTTAAGACCTACCCAAAGTGCAATAGTTTACATCCAGCAGCTA
GGAAGAGGTTTGCGTAAATATCAAGATAAGAAATTTGTGGTTATCCTTGATTTTATCGCA
AACTATACAAATAACTTTCTGATTGCTGTAGCTTTAAGTGGCGATAATAGTTATGATAAG
GACGAGCTTAAAAAGTTTGTCGTATCACCAAATAATTACCTGGCTGGTAAAAGTACCATC
ACTTTTAGTAATATTGCCAAAGAGATTATTTATAAGAATATTCAAAAAACAAATTTCTCA
CAGCTAAAAAATATCAAAAGAGACTACACTCAACTAAAAAAAGAGCTTGGTAGAATCCCA
AATTTAGTAGATTTTCAGCACTATAACTTTATTTCTCCAGAAGTTATCTTATCTACCAAA
GATACTTACTATGATGTTCTAAAGGCTTTTAAAGAGGATATTATTAACTTAGATATACGT
CAGTACAACATCTTAAAGTTTCTTTCAAAAGAATTTACTCCAGCGAAAAGACTTTATGAA
ATATTGATCTTAGAAGAGCTTTTTAAATCTAACTCTATAAGTATATCCGAGCTTGAAACC
TCATTAAAAGCTAGTTTAGTTGACTTTGAAAAGACTAGTTTTACTAATGCGTTATTACAT
CTATCTTTGCAGATATTTACTATTAATGCAGGTAGACAAGATTATGAGCCGTTAATATCA
GTAAATGCTCAAGAGGTTAGTTTGTTGGTTGCTGACTTAATAAATAAGAATAGTTTTTTG
TATAAACAAATTATCGACTTAATCGCTTACAATAAGCTGATTTATGCTAGAAAATACTCT
CAAAATAAATCTCATGGATTAGCGATGTATGCAAAATATACTAAAAAAGAAATTGCTCAT
TTGCTTAACCAAGATTATACAAATGGGGGCGTAAACCTTGCTGGATATAGAGCATTTGGT
AGCAAAGCACTGTTATTTATGACATTTGATGAGTATAAAAAATTTGGATTATATGATAAT
AAATTCATCTCACAACAGGCTTTCTCATACTATTCTAGAGCTGCAAAAACACTTGATGAT
CCTCTAGAAAAAGCACTTGCTGAAAATAGCTATCAAAGTTATGTTTTTGCTAGAATAAAT
AAGCATGATAGTTATTTTTTTCTAGGATTGGTATCTAAATGTTTAGAGGCTAAAGAGATA
AATGCTAGCAAAAAACTTGTTAGCTATATTTTTGAGCTAGAAAAGCCAATTCCTAAAGAA
ATCTGGCAGTATTTTACAACAATTTATAAAAAAAAAATTTAG
Protein sequence predicted based on nucleotide sequence
MIQDSIQFLTNSRKSKILDEIINQFENCEEFIISVAFITFSGVVCILEVLRQLRAKGIKG KILTGCYLNFTEPKALKKLLEFDNIELKILNDENFHAKGFFFRNKDSWQIILGSSNLTQS ALTINNEWNLLIKTSLADVTTQQILAEFDYLFDRAKYPSDILDEYIQIYQRASKLANTSI QRISHKIQPNKIQCEALESLRQLRDSGKNKALIISATGTGKTFLSAFDIASIKPKRCLFV VHRTNIAIKAQETFAKVISDKTLGLYTGGNKTSAEYLFATVQTLKNSKVLANFKSQEFEY IIIDEVHHAEAESYKKVLAHFTPKFLLGMTATPERSDAADIFKLFDYNIAYEIRLHQALE QDLLCPFHYFAVEDLYLESEEILAKNFAKLVSDVRVEHIIEKINFYGYSGQSRAALMFVS NVDEAKELATKFSNKGIKAKALSSQDSEQIRQTTIAQLESGELEIIVTVDIFNEGVDIPC INQIILLRPTQSAIVYIQQLGRGLRKYQDKKFVVILDFIANYTNNFLIAVALSGDNSYDK DELKKFVVSPNNYLAGKSTITFSNIAKEIIYKNIQKTNFSQLKNIKRDYTQLKKELGRIP NLVDFQHYNFISPEVILSTKDTYYDVLKAFKEDIINLDIRQYNILKFLSKEFTPAKRLYE ILILEELFKSNSISISELETSLKASLVDFEKTSFTNALLHLSLQIFTINAGRQDYEPLIS VNAQEVSLLVADLINKNSFLYKQIIDLIAYNKLIYARKYSQNKSHGLAMYAKYTKKEIAH LLNQDYTNGGVNLAGYRAFGSKALLFMTFDEYKKFGLYDNKFISQQAFSYYSRAAKTLDD PLEKALAENSYQSYVFARINKHDSYFFLGLVSKCLEAKEINASKKLVSYIFELEKPIPKE IWQYFTTIYKKKI*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2742
Mutations:
None
Discrepancy:
a17t,K6I; t2733a,N911K
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDEST17
Synonyms:
pDEST-17
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and ribosome binding site (RBS), adds N-term 6xHis tag; amp resistance; recombinational cloning.
Comments:
None
Size (bp):
6354
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, in vitro transcription, recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
ATG
initiation ATG (before 6xHis tag)
101
103
bacterial origin
pBR322 ori
pBR322 origin of replication
3575
4248
gene fragment
ROP
ROP ORF
4619
4810
negative selection marker
ccdB
ccdB death cassette ONLY in Non-Recombined (NO INSERT) form
1374
1679
promoter
T7
T7 promoter
21
40
promoter
bla
bla promoter (prokaryotic promoter from bla gene)
2471
2569
recombination site
attR1
attR1 recombination site
140
264
recombination site
attR2
attR2 recombination site
1720
1844
ribosome binding site
RBS
Ribosome binding site
86
92
selectable marker
CmR
chlorampenicol resistance gene ONLY In Non-Recombined (NO INSERT) form
373
1032
selectable marker
AmpR
ampicillin resistance gene (bla)
2570
3430
tag
His
6xHis tag (N-terminal to insert)
113
130
trxn termination sequence
T7 term
T7 transcription termination sequence
1855
1983
