RHOBTB3 (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  RHOBTB3
Gene Name:  Rho-related BTB domain containing 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Insert size was verirified by PCR
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Arizona State University
Xiaobo Yu
Andrea Throop
Han Li
Joshua LaBaer
HsCD00632733
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1833nts
Open reading frame: 1 to 1830

ATGTCCATCCACATCGTGGCGCTGGGGAACGAGGGGGACACATTCCACCAGGACAACCAG
CCGTCGGGGCTTATCCGCACTTACCTGGGGAGAAGCCCTCTGGTCTCCGGGGACGAGAGC
AGCTTGTTGCTGAACGCGGCCAGCACGGTCGCGCGTCCGGTGTTCACCGAGTATCAGGCC
AGTGCGTTTGGGAATGTCAAGCTGGTGGTCCACGACTGTCCCGTCTGGGACATATTTGAC
AGTGATTGGTACACTTCTCGAAATCTAATTGGGGGCGCTGACATCATTGTGATCAAATAC
AACGTTAATGACAAGTTTTCATTCCATGAAGTAAAGGATAATTATATTCCAGTGATAAAA
AGAGCATTAAATTCAGTTCCAGTAATTATTGCTGCTGTTGGTACCAGACAAAATGAAGAG
TTACCTTGTACATGCCCACTATGTACCTCAGACAGAGGGAGCTGTGTTAGTACAACTGAA
GGGATCCAACTTGCAAAAGAACTAGGAGCAACCTATCTTGAACTCCACAGCCTTGATGAC
TTCTACATAGGAAAGTATTTTGGAGGAGTGTTGGAGTATTTTATGATTCAAGCCTTAAAT
CAGAAGACAAGTGAAAAAATGAAGAAAAGAAAAATGAGCAACTCCTTTCATGGAATTAGA
CCACCTCAACTTGAACAACCAGAAAAAATGCCTGTCTTAAAGGCTGAAGCGTCACATTAT
AACTCTGACTTAAATAACTTGCTGTTCTGCTGCCAGTGTGTGGACGTGGTATTTTATAAC
CCCGATTTAAAGAAAGTTGTAGAGGCCCACAAGATCGTTCTCTGCGCTGTAAGCCATGTT
TTCATGCTGCTTTTCAATGTGAAGAGTCCCACTGACATTCAGGATTCCAGTATCATCCGA
ACTACCCAGGATCTTTTTGCTATAAACAGAGATACTGCATTTCCAGGTGCTAGCCATGAA
TCTTCAGGCAACCCACCATTACGAGTCATTGTTAAAGACGCCCTCTTCTGTTCTTGTTTA
TCAGACATCCTTCGCTTCATTTATTCAGGTGCTTTTCAGTGGGAAGAATTGGAAGAAGAT
ATCAGGAAGAAGTTGAAAGATTCTGGGGATGTTTCAAATGTAATCGAGAAAGTTAAATGC
ATTTTAAAAACACCAGGAAAGATTAATTGCCTAAGGAATTGCAAAACCTATCAAGCCAGA
AAACCTTTGTGGTTTTATAACACTTCCCTCAAGTTTTTCCTTAATAAGCCGATGCTTGCC
GATGTTGTCTTCGAAATTCAAGGTACGACAGTGCCAGCCCACAGGGCCATCCTGGTGGCC
CGTTGTGAAGTGATGGCAGCCATGTTTAATGGTAATTACATGGAAGCAAAGAGTGTCCTG
ATTCCCGTTTATGGTGTTTCCAAAGAGACTTTCTTGTCATTTTTAGAATACCTGTACACA
GACTCCTGCTGCCCAGCTGGCATATTCCAGGCCATGTGTCTCCTGATCTGTGCCGAGATG
TACCAAGTGTCCAGACTGCAGCACATCTGTGAGCTGTTCATCATTACCCAGCTGCAGAGC
ATGCCAAGCAGGGAACTGGCATCCATGAACCTTGATATAGTTGACCTGCTTAAAAAGGCC
AAGTTTCACCACTCTGATTGCCTTTCAACCTGGCTACTTCATTTCATTGCTACTAACTAC
CTCATCTTCAGTCAAAAGCCTGAATTTCAGGATCTTTCAGTGGAAGAACGCAGTTTTGTT
GAAAAGCACAGATGGCCGTCGAATATGTACTTGAAGCAGCTTGCGGAATACAGGAAGTAT
ATTCACTCCCGGAAATGTCGTTGCTTAGTAATG

Protein sequence predicted based on nucleotide sequence

MSIHIVALGNEGDTFHQDNQPSGLIRTYLGRSPLVSGDESSLLLNAASTVARPVFTEYQA
SAFGNVKLVVHDCPVWDIFDSDWYTSRNLIGGADIIVIKYNVNDKFSFHEVKDNYIPVIK
RALNSVPVIIAAVGTRQNEELPCTCPLCTSDRGSCVSTTEGIQLAKELGATYLELHSLDD
FYIGKYFGGVLEYFMIQALNQKTSEKMKKRKMSNSFHGIRPPQLEQPEKMPVLKAEASHY
NSDLNNLLFCCQCVDVVFYNPDLKKVVEAHKIVLCAVSHVFMLLFNVKSPTDIQDSSIIR
TTQDLFAINRDTAFPGASHESSGNPPLRVIVKDALFCSCLSDILRFIYSGAFQWEELEED
IRKKLKDSGDVSNVIEKVKCILKTPGKINCLRNCKTYQARKPLWFYNTSLKFFLNKPMLA
DVVFEIQGTTVPAHRAILVARCEVMAAMFNGNYMEAKSVLIPVYGVSKETFLSFLEYLYT
DSCCPAGIFQAMCLLICAEMYQVSRLQHICELFIITQLQSMPSRELASMNLDIVDLLKKA
KFHHSDCLSTWLLHFIATNYLIFSQKPEFQDLSVEERSFVEKHRWPSNMYLKQLAEYRKY
IHSRKCRCLV

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable