Gene:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nGST_DC
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Insert size was verirified by PCR
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Arizona State University
Xiaobo Yu
Andrea Throop
Lerys del Moral
Joshua LaBaer
Arizona State University
Xiaobo Yu
Andrea Throop
Lerys del Moral
Joshua LaBaer
HsCD00632813
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 576nts
Open reading frame: 1 to 576
ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG
ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT
TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA
CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT
TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG
GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG
GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGATTCCCATCACCTATCCG
CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC
ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT
CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTG
Protein sequence predicted based on nucleotide sequence
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPP PVKKRKRKCLLL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nGST_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
Vector has tendency to recombine between repeat sequences that flank the att sites resulting in decreased LR efficiency
Size (bp):
5998
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3283
3965
bacterial origin
M13 Ori
M13 origin
5359
5814
death cassette
ccdB
ccdB death cassette (removed in clones containing an insert)
2479
2784
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
GSTseqF forward sequencing primer gcaagtatatagcatggcct
1105
1124
promoter
T7
T7 promoter
2982
5998
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
1209
1229
recombination site
attR1
attR1 recombinational cloning site
1252
1269
recombination site
attR2
attR2 recombinational cloning site
2925
2942
reporter gene
LacZ
LacZ alpha
5819
5887
selectable marker
CmR
Chloramphenicol resistance (removed in plasmids containing an insert)
1478
2134
selectable marker
AmpR
Ampicillin resistance marker
4063
4772
ssDNA origin
f1
f1 origin
5491
5797
tag
GST
N-terminal TEV-cleavable GST tag
525
1190
trxn termination sequence
T7 term
T7 terminator
3054
3101
Gene
Gene Symbol :
RAC1
Symbol Nomenclature :
RAC1
SYNONYM :
Rac-1; TC-25; p21-Rac1
Designation :
cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1
Full Nomenclature :
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GENEID :
Locus Tag :
MIG5
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
Alpha4 beta1 integrin signaling events
NCI :
Alpha9 beta1 integrin signaling events
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
Arf1 pathway
NCI :
Arf6 downstream pathway
NCI :
BCR signaling pathway
NCI :
CDC42 signaling events
NCI :
Class I PI3K signaling events
NCI :
CXCR4-mediated signaling events
NCI :
E-cadherin signaling in keratinocytes
NCI :
E-cadherin signaling in the nascent adherens junction
NCI :
Endothelins
NCI :
EPHA2 forward signaling
NCI :
EPHB forward signaling
NCI :
Ephrin B reverse signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL6-mediated signaling events
NCI :
Integrin-linked kinase signaling
NCI :
Integrins in angiogenesis
NCI :
Lissencephaly gene (LIS1) in neuronal migration and development
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
N-cadherin signaling events
NCI :
Nectin adhesion pathway
NCI :
Nephrin/Neph1 signaling in the kidney podocyte
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Noncanonical Wnt signaling pathway
NCI :
Nongenotropic Androgen signaling
NCI :
Osteopontin-mediated events
NCI :
p38 MAPK signaling pathway
NCI :
p75(NTR)-mediated signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Posttranslational regulation of adherens junction stability and dissassembly
NCI :
RAC1 signaling pathway
NCI :
Regulation of p38-alpha and p38-beta
NCI :
Regulation of RAC1 activity
NCI :
S1P1 pathway
NCI :
S1P2 pathway
NCI :
S1P3 pathway
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PRL
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-4-mediated signaling events
NCI :
Thromboxane A2 receptor signaling
NCI :
Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling
Panther :
Axon guidance mediated by netrin
Panther :
Axon guidance mediated by semaphorins
Panther :
Axon guidance mediated by Slit/Robo
Panther :
B cell activation
Panther :
Cytoskeletal regulation by Rho GTPase
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Huntington disease
Panther :
Inflammation mediated by chemokine and cytokine signaling pathway
Panther :
Integrin signalling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
VEGF signaling pathway
Reactome :
Activation of Rac
Reactome :
Adaptive Immune System
Reactome :
Axon guidance
Reactome :
beta-catenin independent WNT signaling
Reactome :
CD28 co-stimulation
Reactome :
CD28 dependent Vav1 pathway
Reactome :
Cell death signalling via NRAGE, NRIF and NADE
Reactome :
Cell-Cell communication
Reactome :
Costimulation by the CD28 family
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
DCC mediated attractive signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
DSCAM interactions
Reactome :
Factors involved in megakaryocyte development and platelet production
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Fcgamma receptor (FCGR) dependent phagocytosis
Reactome :
G alpha (12/13) signalling events
Reactome :
GPCR downstream signaling
Reactome :
GPVI-mediated activation cascade
Reactome :
Hemostasis
Reactome :
HIV Infection
Reactome :
Host Interactions of HIV factors
Reactome :
Immune System
Reactome :
Inactivation of Cdc42 and Rac
Reactome :
Innate Immune System
Reactome :
L1CAM interactions
Reactome :
Membrane Trafficking
Reactome :
Nef and signal transduction
Reactome :
Netrin-1 signaling
Reactome :
NRAGE signals death through JNK
Reactome :
p75 NTR receptor-mediated signalling
Reactome :
PCP/CE pathway
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Regulation of actin dynamics for phagocytic cup formation
Reactome :
Sema3A PAK dependent Axon repulsion
Reactome :
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion
Reactome :
Sema4D in semaphorin signaling
Reactome :
Sema4D induced cell migration and growth-cone collapse
Reactome :
Sema4D mediated inhibition of cell attachment and migration
Reactome :
Semaphorin interactions
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by GPCR
Reactome :
Signaling by Robo receptor
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
The role of Nef in HIV-1 replication and disease pathogenesis
Reactome :
Translocation of GLUT4 to the plasma membrane
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
Androgen receptor signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response (only ATM dependent)
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Focal Adhesion
WikiPathway :
G13 Signaling Pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
NOD pathway
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Physiological and Pathological Hypertrophy of the Heart
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TOR signaling
WikiPathway :
TWEAK Signaling Pathway
WikiPathway :
Wnt Signaling Pathway
Protein
HPRD :
03627
Clone
Cloning Information :
Organism
Species Specific ID:
Genome