Gene:
Gene Symbol:
RHOBTB1
Gene Name:
Rho-related BTB domain containing 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nGST_DC
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Insert size was verirified by PCR
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Arizona State University
Xiaobo Yu
Andrea Throop
Lerys del Moral
Joshua LaBaer
Arizona State University
Xiaobo Yu
Andrea Throop
Lerys del Moral
Joshua LaBaer
HsCD00632823
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2088nts
Open reading frame: 1 to 2088
ATGGACGCTGACATGGACTACGAAAGACCCAACGTTGAAACTATCAAATGTGTGGTCGTG
GGTGACAATGCCGTGGGGAAGACGCGCTTGATCTGTGCCAGGGCGTGCAACACCACACTC
ACGCAGTATCAGCTGCTGGCCACCCACGTGCCAACAGTGTGGGCGATTGACCAGTACCGC
GTGTGCCAGGAGGTCTTGGAGCGTTCTCGGGATGTTGTTGATGAAGTGAGTGTTTCTCTC
AGGCTTTGGGATACTTTTGGTGATCATCACAAAGACAGACGCTTTGCATATGGCAGGTCT
GATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCCCAATTCCCTAAATCATGTGAAAAGC
ATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCGAACACCCGTTATCCTTGTTGGGTGC
CAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGTTAATCGAGCCAGGCGCCCGTTAGCA
AGGCCCATAAAGAGAGGGGATATTTTGCCCCCAGAAAAAGGCCGAGAGGTAGCAAAGGAA
CTTGGCTTACCATACTATGAAACAAGCGTGTTTGACCAGTTTGGTATCAAGGATGTGTTT
GACAATGCAATCCGAGCAGCGCTGATTTCCCGCAGGCACCTGCAATTCTGGAAATCCCAC
CTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACCCTTCCTACCTCCAAAAGCCCCTCCA
CCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGGGACAAATGAAGCTGCCTGTTTACTG
GACAATCCTCTATGTGCCGATGTTCTGTTCATCCTTCAGGACCAGGAACACATCTTTGCA
CATCGAATTTACCTCGCTACCTCTTCTTCCAAATTTTATGATCTGTTTTTAATGGAATGT
GAAGAATCCCCAAATGGGAGTGAAGGAGCCTGTGAGAAAGAGAAGCAGAGCAGAGATTTC
CAGGGGCGGATATTGAGTGTCGACCCAGAGGAAGAAAGGGAGGAGGGCCCGCCTAGGATT
CCTCAGGCCGACCAGTGGAAGTCTTCAAACAAGAGCCTGGTGGAGGCTCTGGGGCTGGAA
GCCGAGGGTGCAGTTCCTGAGACACAGACTTTGACCGGATGGAGTAAGGGGTTCATTGGC
ATGCACAGGGAAATGCAAGTCAACCCCATTTCAAAGCGGATGGGGCCCATGACTGTGGTC
AGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCGGACCCTGCTCCAGTTTCTTTATACG
GGACAACTGGATGAAAAGGAAAAGGATTTGGTGGGCCTGGCTCAGATCGCAGAGGTCCTC
GAGATGTTCGATTTGAGGATGATGGTGGAAAACATCATGAACAAGGAAGCCTTCATGAAC
CAGGAGATTACGAAAGCCTTTCACGTAAGGAAAGCCAATCGGATAAAAGAGTGTCTCAGC
AAGGGAACGTTCTCGGACGTGACATTTAAATTGGACGATGGAGCCATCAGTGCCCACAAG
CCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGCCATGTTCGGGGGGTCATTTGTGGAA
AGTGCCAACAGTGAGGTGTATCTCCCGAACATAAACAAGATATCAATGCAAGCAGTATTG
GATTATCTCTATACCAAGCAGTTGTCTCCTAACTTGGATCTGGACCCGCTGGAATTAATT
GCCTTGGCAAACAGATTTTGCCTGCCACACTTGGTTGCACTTGCAGAACAGCATGCCGTT
CAGGAGTTGACCAAAGCCGCCACGAGTGGCGTGGGCATTGACGGAGAAGTGCTCTCTTAC
TTGGAATTGGCTCAGTTTCACAATGCCCACCAGTTGGCCGCCTGGTGTTTGCACCACATC
TGCACCAACTACAACAGTGTATGCTCCAAGTTCCGTAAGGAAATCAAATCAAAATCTGCA
GACAACCAGGAATACTTCGAGCGGCACCGCTGGCCCCCTGTGTGGTACCTGAAGGAAGAA
GATCACTACCAGCGTGTGAAAAGGGAACGAGAGAAGGAAGATATTGCACTAAATAAGCAT
CGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATCTCCAGCAGTGGCC
Protein sequence predicted based on nucleotide sequence
MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYR VCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKS MWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKE LGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPP PVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMEC EESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSNKSLVEALGLE AEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYT GQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLS KGTFSDVTFKLDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVL DYLYTKQLSPNLDLDPLELIALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSY LELAQFHNAHQLAAWCLHHICTNYNSVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEE DHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nGST_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
Vector has tendency to recombine between repeat sequences that flank the att sites resulting in decreased LR efficiency
Size (bp):
5998
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3283
3965
bacterial origin
M13 Ori
M13 origin
5359
5814
death cassette
ccdB
ccdB death cassette (removed in clones containing an insert)
2479
2784
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
GSTseqF forward sequencing primer gcaagtatatagcatggcct
1105
1124
promoter
T7
T7 promoter
2982
5998
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
1209
1229
recombination site
attR1
attR1 recombinational cloning site
1252
1269
recombination site
attR2
attR2 recombinational cloning site
2925
2942
reporter gene
LacZ
LacZ alpha
5819
5887
selectable marker
CmR
Chloramphenicol resistance (removed in plasmids containing an insert)
1478
2134
selectable marker
AmpR
Ampicillin resistance marker
4063
4772
ssDNA origin
f1
f1 origin
5491
5797
tag
GST
N-terminal TEV-cleavable GST tag
525
1190
trxn termination sequence
T7 term
T7 terminator
3054
3101
