Original Clone ID:
None
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00641189
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1080nts
Open reading frame: 1 to 1080
ATGGCGGCGGCGGCGGCGGCGGGCGCGGGCCCGGAGATGGTCCGCGGGCAGGTGTTCGAC
GTGGGGCCGCGCTACACCAACCTCTCGTACATCGGCGAGGGCGCCTACGGCATGGTGTGC
TCTGCTTATGATAATGTCAACAAAGTTCGAGTAGCTATCAAGAAAATCAGCCCCTTTGAG
CACCAGACCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGACAT
GAGAACATCATTGGAATCAATGACATTATTCGAGCACCAACCATCGAGCAAATGAAAGAT
GTATATATAGTACAGGACCTCATGGAAACAGATCTTTACAAGCTCTTGAAGACACAACAC
CTCAGCAATGACCATATCTGCTATTTTCTCTACCAGATCCTCAGAGGGTTAAAATATATC
CATTCAGCTAACGTTCTGCACCGTGACCTCAAGCCTTCCAACCTGCTGCTCAACACCACC
TGTGATCTCAAGATCTGTGACTTTGGCCTGGCCCGTGTTGCAGATCCAGACCATGATCAC
ACAGGGTTCCTGACAGAATATGTGGCCACACGTTGGTACAGGGCTCCAGAAATTATGTTG
AATTCCAAGGGCTACACCAAGTCCATTGATATTTGGTCTGTAGGCTGCATTCTGGCAGAA
ATGCTTTCTAACAGGCCCATCTTTCCAGGGAAGCATTATCTTGACCAGCTGAACCACATT
TTGGGTATTCTTGGATCCCCATCACAAGAAGACCTGAATTGTATAATAAATTTAAAAGCT
AGGAACTATTTGCTTTCTCTTCCACACAAAAATAAGGTGCCATGGAACAGGCTGTTCCCA
AATGCTGACTCCAAAGCTCTGGACTTATTGGACAAAATGTTGACATTCAACCCACACAAG
AGGATTGAAGTAGAACAGGCTCTGGCCCACCCATATCTGGAGCAGTATTACGACCCGAGT
GACGAGCCCATCGCCGAAGCACCATTCAAGTTCGACATGGAATTGGATGACTTGCCTAAG
GAAAAGCTCAAAGAACTAATTTTTGAAGAGACTGCTAGATTCCAGCCAGGATACAGATCT
Protein sequence predicted based on nucleotide sequence
MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFE HQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQH LSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDH TGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHI LGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK RIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
Gene
Gene Symbol :
MAPK1
Symbol Nomenclature :
MAPK1
SYNONYM :
ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK
Designation :
MAP kinase 1|MAP kinase 2|MAP kinase isoform p42|MAPK 2|extracellular signal-regulated kinase 2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
Full Nomenclature :
mitogen-activated protein kinase 1
GENEID :
GenBank Accession :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
ALK1 signaling events
NCI :
Alpha-synuclein signaling
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
Arf6 downstream pathway
NCI :
ATF-2 transcription factor network
NCI :
BCR signaling pathway
NCI :
BMP receptor signaling
NCI :
CDC42 signaling events
NCI :
Cellular roles of Anthrax toxin
NCI :
Ceramide signaling pathway
NCI :
Class IB PI3K non-lipid kinase events
NCI :
CXCR3-mediated signaling events
NCI :
Downstream signaling in naïve CD8+ T cells
NCI :
EGF receptor (ErbB1) signaling pathway
NCI :
Endothelins
NCI :
EPHB forward signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
ErbB4 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
FGF signaling pathway
NCI :
Glucocorticoid receptor regulatory network
NCI :
GMCSF-mediated signaling events
NCI :
IFN-gamma pathway
NCI :
IL2-mediated signaling events
NCI :
Integrins in angiogenesis
NCI :
mTOR signaling pathway
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Nongenotropic Androgen signaling
NCI :
Osteopontin-mediated events
NCI :
PDGFR-beta signaling pathway
NCI :
Presenilin action in Notch and Wnt signaling
NCI :
Ras signaling in the CD4+ TCR pathway
NCI :
Regulation of cytoplasmic and nuclear SMAD2/3 signaling
NCI :
Regulation of Telomerase
NCI :
Retinoic acid receptors-mediated signaling
NCI :
S1P1 pathway
NCI :
S1P2 pathway
NCI :
S1P3 pathway
NCI :
S1P4 pathway
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PRL
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-1-mediated signaling events
NCI :
Syndecan-2-mediated signaling events
NCI :
TRAIL signaling pathway
NCI :
Trk receptor signaling mediated by the MAPK pathway
NCI :
VEGFR1 specific signals
NCI :
VEGFR3 signaling in lymphatic endothelium
Panther :
Alzheimer disease-amyloid secretase pathway
Panther :
Angiogenesis
Panther :
Angiotensin II-stimulated signaling through G proteins and beta-arrestin
Panther :
Apoptosis signaling pathway
Panther :
B cell activation
Panther :
EGF receptor signaling pathway
Panther :
Endothelin signaling pathway
Panther :
FGF signaling pathway
Panther :
Inflammation mediated by chemokine and cytokine signaling pathway
Panther :
Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade
Panther :
Interferon-gamma signaling pathway
Panther :
Interleukin signaling pathway
Panther :
Parkinson disease
Panther :
PDGF signaling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
TGF-beta signaling pathway
Panther :
VEGF signaling pathway
Reactome :
Activated TLR4 signalling
Reactome :
Activation of NMDA receptor upon glutamate binding and postsynaptic events
Reactome :
Activation of the AP-1 family of transcription factors
Reactome :
Advanced glycosylation endproduct receptor signaling
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Ca-dependent events
Reactome :
Cell Cycle
Reactome :
Cell Cycle, Mitotic
Reactome :
Cellular response to heat stress
Reactome :
Cellular responses to stress
Reactome :
Cellular Senescence
Reactome :
CREB phosphorylation through the activation of Ras
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling of activated FGFR
Reactome :
ERK activation
Reactome :
ERK/MAPK targets
Reactome :
ERK2 activation
Reactome :
ERKs are inactivated
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Fcgamma receptor (FCGR) dependent phagocytosis
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
G-protein mediated events
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
Golgi Cisternae Pericentriolar Stack Reorganization
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
Growth hormone receptor signaling
Reactome :
Hemostasis
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin-2 signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
L1CAM interactions
Reactome :
M Phase
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MAPK targets/ Nuclear events mediated by MAP kinases
Reactome :
Mitotic M-M/G1 phases
Reactome :
Mitotic Prophase
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
NCAM signaling for neurite out-growth
Reactome :
Negative regulation of FGFR signaling
Reactome :
Neuronal System
Reactome :
Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Nuclear Events (kinase and transcription factor activation)
Reactome :
Oncogene Induced Senescence
Reactome :
Opioid Signalling
Reactome :
Oxidative Stress Induced Senescence
Reactome :
phospho-PLA2 pathway
Reactome :
Platelet activation, signaling and aggregation
Reactome :
PLC beta mediated events
Reactome :
Post NMDA receptor activation events
Reactome :
Prolonged ERK activation events
Reactome :
RAF/MAP kinase cascade
Reactome :
Recycling pathway of L1
Reactome :
Regulation of actin dynamics for phagocytic cup formation
Reactome :
Regulation of HSF1-mediated heat shock response
Reactome :
RSK activation
Reactome :
Senescence-Associated Secretory Phenotype (SASP)
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal attenuation
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Spry regulation of FGF signaling
Reactome :
Thrombin signalling through proteinase activated receptors (PARs)
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
Transmission across Chemical Synapses
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
Alzheimers Disease
WikiPathway :
Angiogenesis
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response (only ATM dependent)
WikiPathway :
EBV LMP1 signaling
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
EPO Receptor Signaling
WikiPathway :
ErbB signaling pathway
WikiPathway :
Estrogen signaling pathway
WikiPathway :
Focal Adhesion
WikiPathway :
FSH signaling pathway
WikiPathway :
Heart Development
WikiPathway :
Hypothetical Network for Drug Addiction
WikiPathway :
IL-1 signaling pathway
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
IL-4 signaling pathway
WikiPathway :
IL-5 signaling pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
IL-7 signaling pathway
WikiPathway :
IL-9 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK Cascade
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
Nifedipine Activity
WikiPathway :
Osteopontin Signaling
WikiPathway :
Physiological and Pathological Hypertrophy of the Heart
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
Senescence and Autophagy
WikiPathway :
Signal Transduction of S1P Receptor
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TSH signaling pathway
WikiPathway :
TWEAK Signaling Pathway
WikiPathway :
Type II diabetes mellitus
Protein
HPRD :
01496
Clone
Cloning Information :
IMAGE ID :
4685557
Organism
Species Specific ID:
Genome