EIF2B5 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  EIF2B5
Gene Name:  eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Original Clone ID:
HR8928-1-518-15.10
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00650620
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1557nts
Open reading frame: 1 to 1557

ATGGCGGCCCCTGTAGTGGCGCCGCCTGGTGTGGTGGTTAGTCGGGCTAACAAGCGCAGC
GGCGCGGGGCCGGGAGGCAGCGGTGGCGGGGGAGCCAGAGGGGCGGAGGAGGAACCGCCG
CCGCCCCTACAAGCAGTTCTGGTGGCCGATAGCTTCGATCGCCGCTTCTTCCCCATCTCC
AAGGACCAGCCTCGGGTCCTCTTGCCCCTGGCCAATGTGGCATTAATTGACTACACTCTG
GAATTCCTGACTGCCACAGGTGTACAGGAAACATTTGTCTTTTGTTGCTGGAAAGCTGCT
CAAATCAAAGAACATTTACTGAAGTCAAAGTGGTGCCGCCCTACATCTCTCAATGTGGTT
CGAATAATTACATCAGAGCTCTATCGATCACTGGGAGATGTCCTCCGTGATGTTGATGCC
AAGGCTTTGGTGCGCTCTGACTTTCTTCTGGTGTATGGGGATGTCATCTCAAACATCAAT
ATCACCAGAGCCCTTGAGGAACACAGGTTGAGACGGAAGCTAGAAAAAAATGTTTCTGTG
ATGACGATGATCTTCAAGGAGTCATCCCCCAGCCACCCAACTCGTTGCCACGAAGACAAT
GTGGTAGTGGCTGTGGATAGTACCACAAACAGGGTTCTCCATTTTCAGAAGACCCAGGGT
CTCCGGCGTTTTGCATTTCCTCTGAGCCTGTTTCAGGGCAGTAGTGATGGAGTGGAGGTT
CGATATGATTTACTGGATTGTCATATCAGCATCTGTTCTCCTCAGGTGGCACAACTCTTT
ACAGACAACTTTGACTACCAAACTCGAGATGACTTTGTGCGAGGTCTCTTAGTGAATGAG
GAGATCCTAGGGAACCAGATCCACATGCACGTAACAGCTAAGGAATATGGTGCCCGTGTC
TCCAACCTACACATGTACTCAGCTGTCTGTGCTGACGTCATCCGCCGATGGGTCTACCCT
CTCACCCCAGAGGCGAACTTCACTGACAGCACCACCCAGAGCTGCACTCATTCCCGGCAC
AACATCTACCGAGGGCCTGAGGTCAGCCTGGGCCATGGCAGCATCCTAGAGGAAAATGTG
CTCCTGGGCTCTGGCACTGTCATTGGCAGCAATTGCTTTATCACCAACAGTGTCATTGGC
CCCGGCTGCCACATTGGTGATAACGTGGTGCTGGACCAGACCTACCTGTGGCAGGGTGTT
CGAGTGGCGGCTGGAGCACAGATCCATCAGTCTCTGCTTTGTGACAATGCTGAGGTCAAG
GAACGAGTGACACTGAAACCACGCTCTGTCCTCACTTCCCAGGTGGTCGTGGGCCCAAAT
ATCACGCTGCCTGAGGGCTCGGTGATCTCTTTGCACCCTCCAGATGCAGAGGAAGATGAA
GATGATGGCGAGTTCAGTGATGATTCTGGGGCTGACCAAGAAAAGGACAAAGTGAAGATG
AAAGGTTACAATCCAGCAGAAGTAGGAGCTGCTGGCAAGGGCTACCTCTGGAAAGCTGCA
GGCATGAACATGGAGGAAGAGGAGGAACTGCAGCAGAATCTGTGGGGACTCAAGTAG

Protein sequence predicted based on nucleotide sequence

MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPIS
KDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVV
RIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSV
MTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEV
RYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARV
SNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENV
LLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVK
ERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKM
KGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR8928
Recommended expression in:
Not Applicable