DNASU Plasmid | EP300 (Homo sapiens) in pET15Nano6HT

Explanation of Terms

Gene:

Gene Symbol: EP300

Gene Name: E1A binding protein p300

Original Clone ID:

HR4540G-323-423-TEV.1

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15Nano6HT_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00650691

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 306nts

Open reading frame: 7 to 312

AGCCATATGGGTTCTGGAGCACATACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAG
CAGCTTGTTCTCCTTTTGCATGCTCACAAGTGCCAGCGCCGGGAACAGGCCAATGGGGAA
GTGAGGCAGTGCAACCTTCCCCACTGTCGCACAATGAAGAATGTCCTAAACCACATGACA
CACTGCCAGTCAGGCAAGTCTTGCCAAGTGGCACACTGTGCATCTTCTCGACAAATCATT
TCACACTGGAAGAATTGTACAAGACATGATTGTCCTGTGTGTCTCCCCCTCAAAAATGCT
GGTGATAAGTAG

Protein sequence predicted based on nucleotide sequence

MGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHC
QSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR4540G

Recommended expression in:

Not Applicable

Vector Name:

pET15Nano6HT_NESG

Synonyms:

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial expression withT7 lac promoter, N-terminal Nano-tag, 6XHis-tag, TEV-cleavable; ampicillin resistance in bacteria; restriction enzyme cloning

Comments:

None

Size (bp):

5783

Parent Vector:

None

Properties:

bacterial expression, multiple cloning site, with tag/fusion/marker

Author Name:

Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTCTGAAAATACAGGTTTTCGTGATGGTGATGGTGATGGGTTTCAACCAGCGGAACACGTTCGTCCAGCCAAGCTTCAACGTCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

3938

4620

promoter

T7 promoter

535

554

protease cleavage site

TEV

TEV protease cleavage site

384

401

repressor binding site

lac operator

513

535

repressor protein gene

LacI

LacI repressor

944

2023

selectable marker

AmpR

Ampicillin resistance

4718

5377

tag

His

N-terminal 6xHis tag

402

419

tag

Nano

N-termianl streptavidin-binding Nano tag

420

464

Gene

Gene Symbol :

EP300

Symbol Nomenclature :

EP300

SYNONYM :

KAT3B; RSTS2; p300

Designation :

E1A-associated protein p300|E1A-binding protein, 300kD|histone acetyltransferase p300|p300 HAT

Full Nomenclature :

E1A binding protein p300

GENEID :

2033

Locus Tag :

RP1-85F18.1

GI :

50345997

GenBank Accession :

NM_001429

HGNC :

3373

MIM :

602700

Vega :

OTTHUMG00000150937

Target GenBank:

None

Pathways

NCI :

AP-1 transcription factor network

NCI :

ATF-2 transcription factor network

NCI :

C-MYB transcription factor network

NCI :

Direct p53 effectors

NCI :

E2F transcription factor network

NCI :

FOXA1 transcription factor network

NCI :

FOXM1 transcription factor network

NCI :

FoxO family signaling

NCI :

Glucocorticoid receptor regulatory network

NCI :

HIF-1-alpha transcription factor network

NCI :

HIF-2-alpha transcription factor network

NCI :

IFN-gamma pathway

NCI :

Notch signaling pathway

NCI :

Notch-mediated HES/HEY network

NCI :

p53 pathway

NCI :

p73 transcription factor network

NCI :

Regulation of Androgen receptor activity

NCI :

Regulation of nuclear beta catenin signaling and target gene transcription

NCI :

Regulation of nuclear SMAD2/3 signaling

NCI :

Regulation of retinoblastoma protein

NCI :

Retinoic acid receptors-mediated signaling

NCI :

Role of Calcineurin-dependent NFAT signaling in lymphocytes

NCI :

Signaling events mediated by HDAC Class I

NCI :

Signaling events mediated by HDAC Class III

NCI :

Validated nuclear estrogen receptor alpha network

NCI :

Validated targets of C-MYC transcriptional activation

NCI :

Validated targets of C-MYC transcriptional repression

NCI :

Validated transcriptional targets of AP1 family members Fra1 and Fra2

NCI :

Validated transcriptional targets of TAp63 isoforms

Panther :

Huntington disease

Panther :

p53 pathway

Panther :

TGF-beta signaling pathway

Panther :

Transcription regulation by bZIP transcription factor

Panther :

Wnt signaling pathway

Reactome :

Attenuation phase

Reactome :

BMAL1:CLOCK,NPAS2 activates circadian gene expression

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Cellular response to heat stress

Reactome :

Cellular response to hypoxia

Reactome :

Cellular responses to stress

Reactome :

Chromatin modifying enzymes

Reactome :

Chromatin organization

Reactome :

Circadian Clock

Reactome :

Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants

Reactome :

Constitutive Signaling by NOTCH1 PEST Domain Mutants

Reactome :

Cytosolic sensors of pathogen-associated DNA

Reactome :

Developmental Biology

Reactome :

Disease

Reactome :

Factors involved in megakaryocyte development and platelet production

Reactome :

Fatty acid, triacylglycerol, and ketone body metabolism

Reactome :

FBXW7 Mutants and NOTCH1 in Cancer

Reactome :

formation of the beta-catenin:TCF transactivating complex

Reactome :

G2/M Transition

Reactome :

HATs acetylate histones

Reactome :

Hemostasis

Reactome :

HSF1-dependent transactivation

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

Mitotic G2-G2/M phases

Reactome :

NOTCH1 Intracellular Domain Regulates Transcription

Reactome :

NOTCH2 intracellular domain regulates transcription

Reactome :

Polo-like kinase mediated events

Reactome :

PPARA activates gene expression

Reactome :

Pre-NOTCH Expression and Processing

Reactome :

Pre-NOTCH Transcription and Translation

Reactome :

Regulation of gene expression by Hypoxia-inducible Factor

Reactome :

Regulation of Hypoxia-inducible Factor (HIF) by oxygen

Reactome :

Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Reactome :

REV-ERBA represses gene expression

Reactome :

RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways

Reactome :

RORA activates circadian gene expression

Reactome :

Signal Transduction

Reactome :

Signaling by NOTCH

Reactome :

Signaling by NOTCH1

Reactome :

Signaling by NOTCH1 HD Domain Mutants in Cancer

Reactome :

Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer

Reactome :

Signaling by NOTCH1 in Cancer

Reactome :

Signaling by NOTCH1 PEST Domain Mutants in Cancer

Reactome :

Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant

Reactome :

Signaling by NOTCH2

Reactome :

Signaling by Wnt

Reactome :

TCF dependent signaling in response to WNT

Reactome :

TRAF3-dependent IRF activation pathway

Reactome :

TRAF6 mediated IRF7 activation

Reactome :

Transcriptional regulation of white adipocyte differentiation

WikiPathway :

Androgen receptor signaling pathway

WikiPathway :

Cell cycle

WikiPathway :

Energy Metabolism

WikiPathway :

IL-4 signaling pathway

WikiPathway :

Integrated Breast Cancer Pathway

WikiPathway :

Kit receptor signaling pathway

WikiPathway :

Notch Signaling Pathway

WikiPathway :

SIDS Susceptibility Pathways

WikiPathway :

TGF beta Signaling Pathway

WikiPathway :

TGF Beta Signaling Pathway

WikiPathway :

Wnt Signaling Pathway and Pluripotency

Protein

Structural Annotation Wiki :

HR4540G

UniProt :

Q09472

HPRD :

04078

Clone

Cloning Information :

686657

Original Clone ID :

HR4540G-323-423-TEV.1

Experimental

TargetTrack - Experimental data :

HR4540G

Organism

Species Specific ID:

2033

Genome

Chromosome :

Map Location :

22q13.2

Ensembl :

ENSG00000100393

EP300 (Homo sapiens) in pET15Nano6HT_NESG

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Experimental

Organism

Genome