GCGR (Homo sapiens) in pFASTBAC1

Explanation of Terms

Gene:
Gene Symbol:  GCGR
Gene Name:  glucagon receptor
Original Clone ID:
IMPT-3172
Keyword:
BRIL fusion
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFASTBAC1
Format: 
FUSION
Source:
GPCR Network
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The Scripps Research Institute
Jeffrey Velasquez
PSI:Biology
GPCR Network
HsCD00651513
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1212nts
Open reading frame: 1 to 1212

ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCCGGCGCGCCAGCT
GATCTGGAAGACAATTGGGAAACTCTGAACGACAATCTCAAGGTGATCGAGAAGGCTGAC
AATGCTGCACAAGTCAAAGACGCTCTGACCAAGATGAGGGCAGCAGCCCTGGACGCTCAG
AAGGCCACTCCACCTAAGCTCGAGGACAAGAGCCCAGATAGCCCTGAAATGAAAGACTTT
CGGCATGGATTCGACATTCTGGTGGGACAGATTGATGATGCACTCAAGCTGGCCAATGAA
GGGAAAGTCAAGGAAGCACAAGCAGCCGCTGAGCAGCTGAAGACCACCCGGAATGCATAC
ATTCAGAAGTACCTGATGGACGGTGAGGAAATTGAGGTTCAGAAGGAGGTCGCGAAGATG
TATTCATCTTTCCAAGTAATGTATACCGTCGGCTACTCCTTGTCCCTTGGTGCGCTTCTG
CTTGCACTTGCAATCTTGGGCGGCCTGTCCAAACTCCACTGCACTAGAAACGCCATCCAC
GCTAACTTGTTCGCTTCATTCGTTCTCAAAGCCTCCTCCGTCCTGGTCATTGACGGCCTG
CTTAGGACTAGGTATTCTCAAAAAATCGGTGACGATTTGAGCGTTTCAACTTGGTTGTCC
GATGGAGCGGTGGCAGGATGTCGCGTAGCCGCGGTTTTTATGCAATACGGAATTGTCGCT
AACTACTGTTGGCTTTTGGTTGAGGGCTTGTATCTCCACAACCTTCTGGGCCTCGCGACC
CTTCCCGAGAGATCATTTTTTTCCTTGTATTTGGGCATTGGTTGGGGCGCTCCTATGCTC
TTCGTAGTTCCGTGGGCTGTGGTTAAATGCTTGTTTGAAAACGTGCAGTGCTGGACTTCT
AACGATAACATGGGATTCTGGTGGATTCTCCGCTTCCCAGTTTTCCTGGCTATTCTGATC
AACTTCTTCATATTCGTCAGAATAGTCCAGCTGCTCGTCGCGAAGTTGCGTGCTAGACAA
ATGCATCATACCGACTATAAATTTAGGCTCGCGAAATCTACCCTTACTCTGATCCCGTTG
CTCGGTGTCCACGAGGTCGTGTTCGCCTTTGTCACCGACGAGCATGCCCAGGGCACGCTG
AGATCAGCTAAGTTGTTTTTTGATCTGTTTCTGAGTTCATTTCAGGGACTGCTCGTGGCC
GTACTGTACTGTTTTCTGAACAAGGAAGTTCAATCCGAGCTTCGCAGACGTTGGCATCGT
TGGAGACTCGGAAAAGTGCTTTGGGAGGAGCGTAACACTTCGAACGGCCGGCCTCTGGAA
GTTCTGTTCCAGGGGCCCCATCATCATCATCATCATCATCATCATCATGACTACAAAGAC
GATGACGACAAGTAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFAGAPADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQ
KATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAY
IQKYLMDGEEIEVQKEVAKMYSSFQVMYTVGYSLSLGALLLALAILGGLSKLHCTRNAIH
ANLFASFVLKASSVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVAGCRVAAVFMQYGIVA
NYCWLLVEGLYLHNLLGLATLPERSFFSLYLGIGWGAPMLFVVPWAVVKCLFENVQCWTS
NDNMGFWWILRFPVFLAILINFFIFVRIVQLLVAKLRARQMHHTDYKFRLAKSTLTLIPL
LGVHEVVFAFVTDEHAQGTLRSAKLFFDLFLSSFQGLLVAVLYC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in GPCR-49
Recommended expression in:
Not Applicable