NFS1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  NFS1
Gene Name:  NFS1 cysteine desulfurase
Original Clone ID:
HR9252B-34-457-15.2
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00663960
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1275nts
Open reading frame: 7 to 1281

CATATGGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGCAGATACAGCCGCTGCCCCGGAG
GTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTGGACCCC
CGGGTGCTTGATGCCATGCTCCCTTACCTAATCAACTACTATGGGAACCCACACTCCCGG
ACACATGCTTATGGCTGGGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAAGTAGCA
TCTCTGATTGGAGCTGATCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAATCCAAC
AACATAGCAATTAAGGGGGTGGCCCGATTCTACAGGTCACGGAAAAAGCACTTGATCACC
ACCCAGACAGAACACAAATGTGTCTTGGACTCCTGCCGTTCACTGGAAGCTGAGGGCTTT
CAGGTCACCTACCTCCCAGTGCAGAAGAGTGGGATCATTGACCTAAAGGAACTAGAGGCT
GCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGACTGTGAACAATGAGATTGGAGTG
AAGCAGCCTATTGCAGAAATAGGGCGGATTTGCAGTTCCAGAAAGGTATATTTCCATACT
GATGCAGCCCAGGCTGTTGGAAAAATCCCACTTGATGTCAATGACATGAAAATTGATCTC
ATGAGCATTAGTGGTCACAAAATCTACGGTCCCAAAGGGGTTGGTGCCATCTACATCCGT
CGCCGGCCCCGTGTGCGTGTGGAGGCCCTGCAGAGTGGAGGGGGGCAGGAGCGGGGTATG
CGGTCTGGGACAGTGCCCACACCCTTAGTGGTGGGGCTGGGGGCTGCGTGTGAGGTGGCA
CAGCAAGAGATGGAGTATGACCACAAGCGAATCTCAAAGTTGTCAGAGCGGCTGATACAG
AATATAATGAAGAGCCTTCCAGATGTGGTGATGAATGGGGACCCTAAGCACCATTATCCC
GGCTGTATCAACCTCTCCTTTGCATATGTGGAAGGGGAAAGTCTGCTGATGGCACTGAAG
GACGTTGCCTTATCCTCAGGGAGTGCCTGCACCTCTGCATCCCTGGAGCCCTCTTATGTG
CTTAGAGCAATTGGCACTGATGAGGATTTAGCGCACTCTTCTATCAGGTTTGGAATTGGC
CGCTTCACTACAGAGGAGGAAGTGGACTACACAGTGGAGAAATGCATTCAGCATGTGAAG
CGTCTTCGAGAAATGAGCCCTCTCTGGGAGATGGTTCAGGATGGCATTGACCTCAAGAGC
ATCAAGTGGACCCAACACTAG

Protein sequence predicted based on nucleotide sequence

GDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTH
AYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQ
TEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQ
PIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRR
PRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNI
MKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLR
AIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIK
WTQH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in HR9252B
Recommended expression in:
Not Applicable