NAGS (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  NAGS
Gene Name:  N-acetylglutamate synthase
Original Clone ID:
HR6833-115-534-15.4
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00696775
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1266nts
Open reading frame: 27 to 1292

GCCATCACCATCACCATCACAGCCATATGGCCAGCCCTGGGGAGGCGCGCCACTGGCTCA
CGCAGTTCCAGACCTGCCATCACTCCGCGGACAAGCCCTTCGCCGTCATCGAGGTGGACG
AGGAGGTGCTCAAGCGCCAGCAGGGCGTATCCAGTCTGGCCTTTGCCCTGGCCTTCTTGC
AGCGCATGGACATGAAGCCGCTGGTGGTCCTGGGGCTGCCGGCCCCCACGGCTCCCTCGG
GCTGTCTTTCCTTCTGGGAGGCCAAGGCGCAGCTGGCCAAGAGCTGCAAGGTGCTGGTAG
ACGCGCTTCGACACAACGCCGCCGCTGCTGTGCCATTTTTTGGCGGCGGGTCTGTGCTAC
GCGCTGCCGAGCCGGCTCCCCATGCCAGCTACGGCGGCATCGTCTCGGTGGAGACAGACC
TGCTGCAGTGGTGCCTGGAGTCGGGCAGCATCCCCATCCTGTGCCCCATCGGGGAGACGG
CCGCGCGCCGCTCCGTGCTTCTCGACTCCCTGGAGGTGACCGCGTCGCTGGCCAAGGCGC
TGCGGCCCACCAAAATCATCTTCCTCAATAACACAGGCGGCCTGCGCGACAGCAGTCATA
AGGTCCTGAGTAACGTGAACCTGCCCGCCGACCTGGACCTGGTGTGCAACGCCGAGTGGG
TGAGCACAAAAGAACGGCAGCAGATGCGGCTCATCGTGGACGTGCTCAGCCGCCTACCCC
ACCACTCCTCGGCCGTCATCACCGCCGCTAGCACGCTGCTCACTGAGCTCTTTAGCAACA
AGGGGTCCGGGACCCTGTTCAAGAACGCCGAGCGAATGCTACGGGTGCGCAGCCTGGACA
AGCTGGACCAGGGCCGTCTAGTGGACCTGGTCAACGCCAGCTTCGGCAAGAAGCTCAGGG
ACGACTACCTGGCCTCGCTGCGCCCGCGGCTGCACTCCATCTACGTCTCCGAGGGGTACA
ACGCCGCCGCCATTCTGACCATGGAGCCCGTCCTGGGGGGCACCCCGTACCTGGACAAAT
TTGTGGTGAGCTCCAGCCGCCAGGGCCAAGGCTCCGGCCAGATGCTGTGGGAGTGCCTGC
GGCGGGACCTTCAGACACTTTTCTGGCGCTCCCGGGTCACCAACCCCATCAATCCCTGGT
ACTTCAAACACAGTGATGGCAGCTTCTCCAACAAGCGGTGGATCTTCTTCTGGTTTGGCC
TGGCTGATATCCGGGACTCCTATGAGTTGGTCAACCACGCCAAGGGACTGCCAGACTCCT
TTCACAAGCCAGCTTCTGACCCAGGCAGCTAG

Protein sequence predicted based on nucleotide sequence

MASPGEARHWLTQFQTCHHSADKPFAVIEVDEEVLKRQQGVSSLAFALAFLQRMDMKPLV
VLGLPAPTAPSGCLSFWEAKAQLAKSCKVLVDALRHNAAAAVPFFGGGSVLRAAEPAPHA
SYGGIVSVETDLLQWCLESGSIPILCPIGETAARRSVLLDSLEVTASLAKALRPTKIIFL
NNTGGLRDSSHKVLSNVNLPADLDLVCNAEWVSTKERQQMRLIVDVLSRLPHHSSAVITA
ASTLLTELFSNKGSGTLFKNAERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRP
RLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFW
RSRVTNPINPWYFKHSDGSFSNKRWIFFWFGLADIRDSYELVNHAKGLPDSFHKPASDPG
S*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t109c,C37R;t201c,P67P;

g690a,L230L;a1151g,Q384R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR6833
Recommended expression in:
Not Applicable