Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH248529.01F
Keyword:
None
Species:
Bacillus anthracis str. Ames
Type:
cDNA
Vector Name:
pANT7_cGST
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
BaCD00070787
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1704nts
Open reading frame: 1 to 1704

ATGCTAGAACAAAGAGGTCATGCATTATATGAATCTTCATTGCGAGATTTAAAGTTAGCA
TTAGATGAGTCTTCAATTGTTGCGATTACAGACCGAAAGGGTCTTATTACATATGTAAAT
GATAAGTTTTGTGAAATCTCCAAATATACAAGAGAAGAGTTAATAGGGAAAGATCATCGT
ATTTTAAATTCAGGATATCACCCGAAAACATTTTTTCAAATGCTATGGAAGCGTATTTTG
AGCGGAAAAGTATGGAGAGGTGAGATTCGAAATCAAGCAAAAGATGGTACATATTATTGG
GTTAAAACAACAATTGTTCTATTTTTAAATGAAAAAGGAGAGCCATATCAATTTATCGCG
ATCCGAAATGATATTAGTAGTAGGAAAGAAGCAGAACAGAGGTTGCGGTTAAGCGAAAGT
CGTTATAGGGAGCTGGCTTATCATGATGCTTTAACGAGTTTACCGAACCGTTTACAATTA
ATAACGACTGTTAATGAGTTGATTGCAGATAAAAACGAGTTTGGTATGATTTATTTTGAT
TTGGATCGTTTTAAATTAGTAAACGATACGCTAGGTCATATGATAGGAGATTTACTTTTA
AGCGAAGTTGCTTCACGGTTGCATTATGTGCTAGGAGAGAAAGATGTTCTGGCTAGGCTG
GGTGGCGACGAATTTGTGTTGTTAACAAAAAATCATAAACAAGATGAGATGAGGCAGCTA
GCGACATCTGTGTTGTCATGCTTTCAGGCACCATTTATGCTTGAAGGTCATGAAGTATAC
ATTTCGGCTAGTCTAGGACTGTGCTCATATCCGCAAGATGGACAAGATGTAGAAACGCTA
TTGAAGAATTCGGATTTAGCTATGTATAGCGCGAAAGAACAGGGGAGAAACGCCGCGTGT
TTCTTTACAGATGAGTTACGTGCGAAAATAAATCGTAGAATGAAAATTGAATTTGCTTTG
CAAAAGGCAATTCGTGATGAAGAGTTAGATGTGGTGTTGCAGCCTATTATTGATTTGAAA
ACGAAGAAATATACTGGTATCGAGGCTTTAGTACGTTGTACGACAGAAGAAGGTCCGATT
TCCCCAAGCGAATTTATTCCTATAGCTGAAGAATCTGGACTTATTATAAAATTAGGAGAC
TGGGTGTTAGAAAAGTCATGTCGCTTGTTTAAAACATTGCCTGAGTATGAAGAAGGACTA
AAGTTATCTGTGAATTTATCCATACAACAATTAATGCAAAAGCGTTTTATTATATCTGTA
CGTAGTATTTTAAGGAGAACTGGTTTTCCTGCAAATCGTTTAATTTTAGAAATAACGGAA
AGCATTGCTGTTCGTCATTTTGATTATATTATTGCTACATTACAAGAATTAAGAAATATG
GGTATTTTAATTGCTTTAGATGACTTCGGAACAGGCTATTCTTCCTTATATTATTTAAAA
CAACTACCGTTGGATATAGTAAAAATTGATCGTAATTTTATTCGTGAATTTCATTATGAT
CATGCACAACCAGAGCGAACGATTGTAAAATCTGTTATTGAAATTGCCCATAGTTTAAAT
TTAGCAGTAGTTGCTGAAGGGGTAGAAACAGTAGAGCAAGAAAGCTTATTAAAATCGATG
GGCTGTGATTATGTACAAGGATTTTATTATGCAAAACCTTTATCTGTAGCAGAGTTAAAG
GAAAAGTTAATTATAGACTTCTTG

Protein sequence predicted based on nucleotide sequence

MLEQRGHALYESSLRDLKLALDESSIVAITDRKGLITYVNDKFCEISKYTREELIGKDHR
ILNSGYHPKTFFQMLWKRILSGKVWRGEIRNQAKDGTYYWVKTTIVLFLNEKGEPYQFIA
IRNDISSRKEAEQRLRLSESRYRELAYHDALTSLPNRLQLITTVNELIADKNEFGMIYFD
LDRFKLVNDTLGHMIGDLLLSEVASRLHYVLGEKDVLARLGGDEFVLLTKNHKQDEMRQL
ATSVLSCFQAPFMLEGHEVYISASLGLCSYPQDGQDVETLLKNSDLAMYSAKEQGRNAAC
FFTDELRAKINRRMKIEFALQKAIRDEELDVVLQPIIDLKTKKYTGIEALVRCTTEEGPI
SPSEFIPIAEESGLIIKLGDWVLEKSCRLFKTLPEYEEGLKLSVNLSIQQLMQKRFIISV
RSILRRTGFPANRLILEITESIAVRHFDYIIATLQELRNMGILIALDDFGTGYSSLYYLK
QLPLDIVKIDRNFIREFHYDHAQPERTIVKSVIEIAHSLNLAVVAEGVETVEQESLLKSM
GCDYVQGFYYAKPLSVAELKEKLIIDFL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1704
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable