DNASU Plasmid | ARFGAP3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ARFGAP3

Gene Name: ADP-ribosylation factor GTPase activating protein 3

Original Clone ID:

FLH257573.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00073531

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1548nts

Open reading frame: 1 to 1548

Reference Sequence Alignment

ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCC
ACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTAT
GGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGT
TTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAA
GTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGAC
ACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTC
GCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCA
CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTG
AGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTG
GAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTT
AATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAA
GCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACA
TGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGAC
CTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG
GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTT
GACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTG
ACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAG
TATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGGTACTTTGACGAGCCA
GTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAA
AAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCT
ACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG
TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGAT
TATGAGACCAGGGCCCGCCTAGAGAGGCTGTCAGCAAGTTCCTCCATAAGCTCGGCTGAT
CTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGCGTGCTGCCC
AACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCC
GTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCT

Protein sequence predicted based on nucleotide sequence

MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLS
FIRSTELDSNWSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSL
ASQATRKHGTDLWLDSCVVPPLSPPPKEEDFFASHVSPEVSDTAWASAIAEPSSLTSRPV
ETTLENNEGGQEQGPSVEGLNVPTKATLEVSSIIKKKPNQAKKGLGAKKGSLGAQKLANT
CFNEIEKQAQAADKMKEQEDLAKVVSKEESIVSSLRLAYKDLEIQMKKDEKMNISGKKNV
DSDRLGMGFGNCRSVISHSVTSDMQTIEQESPIMAKPRKKYNDDSDDSYFTSSSRYFDEP
VELRSSSFSSWDDSSDSYWKKETSKDTETVLKTTGYSDRPTARRKPDYEPVENTDEAQKK
FGNVKAISSDMYFGRQSQADYETRARLERLSASSSISSADLFEEPRKQPAGNYSLSSVLP
NAPDMAQFKQGVRSVAGKLSVFANGVVTSIQDRYGS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 12

End on reference sequence 1559

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

Mach1 T1-R

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ARFGAP3

Symbol Nomenclature :

ARFGAP3

SYNONYM :

ARFGAP1

Designation :

ADP-ribosylation factor GTPase activating protein 1|ADP-ribosylation factor GTPase-activating protein 3|ARF GAP 3

Full Nomenclature :

ADP-ribosylation factor GTPase activating protein 3

GENEID :

26286

Locus Tag :

AL049757.1

GI :

109451597

GenBank Accession :

CU013228

HGNC :

661

MIM :

612439

Vega :

OTTHUMG00000150718

Target GenBank:

CR456382

Reference Sequence Alignment

  

HsCD00073531        ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCC
NM_014570.4         ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCC
NM_001142293.1      ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCC
                    ************************************************************

HsCD00073531        ACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTAT
NM_014570.4         ACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTAT
NM_001142293.1      ACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTAT
                    ************************************************************

HsCD00073531        GGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGT
NM_014570.4         GGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGT
NM_001142293.1      GGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGT
                    ************************************************************

HsCD00073531        TTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAA
NM_014570.4         TTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAA
NM_001142293.1      TTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAA
                    ************************************************************

HsCD00073531        GTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGAC
NM_014570.4         GTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGAC
NM_001142293.1      GTCGGAGGAAACGCTAGT------------------------------------------
                    ******************                                          

HsCD00073531        ACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTC
NM_014570.4         ACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTC
NM_001142293.1      ------------------------------------------------------------
                                                                                

HsCD00073531        GCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCA
NM_014570.4         GCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCA
NM_001142293.1      ------------------------------GCACTGTGGCTTGATAGTTGTGTGGTTCCA
                                                  *.:***************************

HsCD00073531        CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTG
NM_014570.4         CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTG
NM_001142293.1      CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTG
                    ************************************************************

HsCD00073531        AGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTG
NM_014570.4         AGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTG
NM_001142293.1      AGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTG
                    ************************************************************

HsCD00073531        GAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTT
NM_014570.4         GAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTT
NM_001142293.1      GAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTT
                    ************************************************************

HsCD00073531        AATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAA
NM_014570.4         AATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAA
NM_001142293.1      AATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAA
                    ************************************************************

HsCD00073531        GCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACA
NM_014570.4         GCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACA
NM_001142293.1      GCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACA
                    ************************************************************

HsCD00073531        TGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGAC
NM_014570.4         TGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGAC
NM_001142293.1      TGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGAC
                    ************************************************************

HsCD00073531        CTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG
NM_014570.4         CTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG
NM_001142293.1      CTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG
                    ************************************************************

HsCD00073531        GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTT
NM_014570.4         GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTT
NM_001142293.1      GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTT
                    ************************************************************

HsCD00073531        GACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTG
NM_014570.4         GACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTG
NM_001142293.1      GACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTG
                    ************************************************************

HsCD00073531        ACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAG
NM_014570.4         ACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAG
NM_001142293.1      ACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAG
                    ************************************************************

HsCD00073531        TATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGGTACTTTGACGAGCCA
NM_014570.4         TATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCA
NM_001142293.1      TATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCA
                    ******************************************** ***************

HsCD00073531        GTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAA
NM_014570.4         GTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAA
NM_001142293.1      GTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAA
                    ************************************************************

HsCD00073531        AAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCT
NM_014570.4         AAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCT
NM_001142293.1      AAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCT
                    ************************************************************

HsCD00073531        ACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG
NM_014570.4         ACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG
NM_001142293.1      ACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG
                    ************************************************************

HsCD00073531        TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGAT
NM_014570.4         TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGAT
NM_001142293.1      TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGAT
                    ************************************************************

HsCD00073531        TATGAGACCAGGGCCCGCCTAGAGAGGCTGTCAGCAAGTTCCTCCATAAGCTCGGCTGAT
NM_014570.4         TATGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGAT
NM_001142293.1      TATGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGAT
                    ********************************.***************************

HsCD00073531        CTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGCGTGCTGCCC
NM_014570.4         CTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCC
NM_001142293.1      CTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCC
                    ************************************************** *********

HsCD00073531        AACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCC
NM_014570.4         AACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCC
NM_001142293.1      AACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCC
                    ************************************************************

HsCD00073531        GTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCT---
NM_014570.4         GTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAA
NM_001142293.1      GTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAA
                    ************************************************