NCAPH2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NCAPH2
Gene Name:  non-SMC condensin II complex, subunit H2
Original Clone ID:
FLH257583.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00073541
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1815nts
Open reading frame: 1 to 1815

ATGGAGGACGTGGAGGCGCGCTTCGCCCACCTCTTGCAGCCCATCCGCGACCTCACCAAG
AACTGGGAGGTGGACGTGGCGGCCCAGCTGGGCGAGTATCTGGAGGAGCTGGATCAGATC
TGCATTTCTTTTGACGAAGGCAAGACCACAATGAACTTCATTGAGGCAGCGTTGTTGATC
CAGGGCTCTGCCTGCGTCTACAGTAAGAAGGTGGAATACCTCTACTCACTCGTCTACCAG
GCCCTTGATTTCATCTCTGGAAAGAGGCGGGCCAAGCAGCTCTCTTCGGTGCAGGAGGAC
AGGGCCAATGGGGTTGCCAGCTCCGGGGTCCCCCAGGAGGCAGAGAATGAGTTCCTGTCG
CTGGATGACTTCCCTGACTCCCGGACTAACGTGGATCTCAAGAATGATCAGACGCCCAGT
GAGGTCCTCATCATCCCCCTCCTGCCCATGGCCCTGGTGGCCCCTGATGAAATGGAGAAG
AACAACAATCCCCTGTACAGCCGTCAGGGTGAGGTCCTGGCCAGCCGGAAGGATTTCAGG
ATGAACACGTGCGTTCCCCACCCCAGAGGGGCCTTCATGTTGGAGCCAGAGGGCATGTCC
CCCATGGAACCAGCGGGCGTTTCCCCCATGCCAGGGACCCAGAAGGACACCGGGAGGACT
GAGGAGCAGCCAATGGAAGTTTCCGTGTGCAGGAGCCCTGTCCCAGCACTCGGCTTCTCC
CAGGAGCCAGGCCCCTCTCCAGAAGGCCCGATGCCCCTGGGTGGGGGCGAGGACGAGGAT
GCAGAGGAGGCAGTAGAGCTTCCTGAGGCCTCGGCCCCCAAGGCCGCTCTGGAGCCCAAG
GAGTCCAGGAGCCCGCAGCAGAGTGCTGCCCTGCCCAGGAGGTACATGCTGCGGGAGCGG
GAGGGGGCCCCAGAGCCTGCATCCTGCGTGAAGGAGACTCCAGACCCCTGGCAGAGCCTG
GACCCCTTTGACTCCTTGGAGTCTAAGCCCTTCAAGAAAGGTAGGCCTTACTCTGTGCCC
CCCTGTGTGGAGGAGGCTCTGGGACAGAAGCGCAAGAGGAAGGGCGCTGCCAAGCTGCAG
GACTTCCACCAGTGGTACCTGGCTGCCTATGCAGACCATGCCGACAGCAGGCGGCTTCGG
CGAAAGGGTCCGTCCTTTGCAGACATGGAGGTCCTGTACTGGACACACGTGAAGGAGCAG
TTGGAAACTCTCCGGAAGCTGCAGAGGAGGGAGGTGGCTGAGCAGTGGCTGCGGCCTGCA
GAGGAGGACCACCTGGAGGATTCCCTGGAAGACCTGGGGGCAGCAGATGACTTTCTAGAG
CCTGAGGAGTACATGGAGCCCGAGGGAGCAGACCCCAGGGAAGCCGCTGACCTTGACGCA
GTGCCGATGTCCCTGAGCTACGAGGAGCTGGTTCGAAGGAATGTGGAGCTCTTCATCGCC
ACCTCCCAGAAGTTTGTCCAGGAGACAGAGCTGAGCCAGCGCATCAGGGACTGGGAGGAC
ACAGTGCAGCCTCTGCTCCAGGAGCAGGAGCAGCATGTGCCCTTTGACATCCACACCTAT
GGGGACCAGCTGGTCTCACGGTTCCCCCAGCTCAATGAGTGGTGTCCCTTTGCGGAGCTG
GTGGCTGGCCAGCCGGCCTTCGAGGTGTGTCGTTCCATGCTGGCCTCCCTGCAGCTGGCC
AATGACTACACAGTGGAGATAACCCAGCAGCCCGGGCTGGAGATGGCCGTGGACACCATG
TCCCTGAGACTGCTCACGCACCAGCGAGCGCACAAGCGCTTCCAGACCTACGCTGCCCCC
TCCATGGCCCAGCCC

Protein sequence predicted based on nucleotide sequence

MEDVEARFAHLLQPIRDLTKNWEVDVAAQLGEYLEELDQICISFDEGKTTMNFIEAALLI
QGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSSVQEDRANGVASSGVPQEAENEFLS
LDDFPDSRTNVDLKNDQTPSEVLIIPLLPMALVAPDEMEKNNNPLYSRQGEVLASRKDFR
MNTCVPHPRGAFMLEPEGMSPMEPAGVSPMPGTQKDTGRTEEQPMEVSVCRSPVPALGFS
QEPGPSPEGPMPLGGGEDEDAEEAVELPEASAPKAALEPKESRSPQQSAALPRRYMLRER
EGAPEPASCVKETPDPWQSLDPFDSLESKPFKKGRPYSVPPCVEEALGQKRKRKGAAKLQ
DFHQWYLAAYADHADSRRLRRKGPSFADMEVLYWTHVKEQLETLRKLQRREVAEQWLRPA
EEDHLEDSLEDLGAADDFLEPEEYMEPEGADPREAADLDAVPMSLSYEELVRRNVELFIA
TSQKFVQETELSQRIRDWEDTVQPLLQEQEQHVPFDIHTYGDQLVSRFPQLNEWCPFAEL
VAGQPAFEVCRSMLASLQLANDYTVEITQQPGLEMAVDTMSLRLLTHQRAHKRFQTYAAP
SMAQP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1869
Start on reference sequence 55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
Mach1 T1-R
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable