MKL1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MKL1
Gene Name:  megakaryoblastic leukemia (translocation) 1
Original Clone ID:
FLH257731.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00073689
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2793nts
Open reading frame: 1 to 2793

ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCC
AGGACAGAGGACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTC
AGGATGCACATTTTGGAAGAGACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAG
CTGAAGAGAGCCAGACTAGCCGATGACCTCAATGAGAAGATTGCACAGAGGCCTGGCCCC
ATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAGTCCAGCCTGAAGGAAGCCATCATT
GTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTCGATGAGGACAGCAGC
GATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCGTCACCC
CTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTT
GTGTCTCAACTTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCT
CCTCTGCCTCCCCCACCTCTGCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACT
GCCAAGTCCACCCCCACACTCATTAAGCAAAGCCAACCCAAGTCTGCCAGTGAGAAGTCA
CAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGAAGAAGCTCAAGTACCACCAG
TACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCCCCCATGGACTCATCCTACGCC
AAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAACCAGCAGCAGCAG
CAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCCCTG
GGAAGCAGCGGGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCG
GGCGCCCCTGGGCCCTGTGGGCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCG
GGAGCCCTGCCGGCCAACCTGGACGACATGAAGGTGGCAGAGCTGAAGCAGGAGCTGAAG
TTGCGATCACTGCCTGTCTCGGGCACCAAAACTGAGCTGATTGAGCGCCTTCGAGCCTAT
CAAGACCAAATCAGCCCTGTGCCAGGAGCCCCCAAGGCCCCTGCCGCCACCTCTATCCTG
CACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGGCTGAGCACGGGGCCAGCC
CTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCCAGCAGTGGG
GTGGTGAAGTTTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGC
TCACTGCTCAGCACGGGCGATGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTG
ACATCACCTCTGACGCAGCTGACCCTGCAGGCCTCGCCACTGCAGATCCTCGTGAAGGAG
GAGGGCCCCCGGGCCGGGTCCTGTTGCCTGAGCCCTGGGGGGCGGGCGGAGCTAGAGGGG
CGCGACAAGGACCAGATGCTGCAGGAGAAAGACAAGCAGATCGAGGCGCTGACGCGCATG
CTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAGCTGCAGCTGGAGCAGGAGAAGCGA
GCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTGAAGCAGGAGAACAGC
TTCTCCAGCTGCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAACCCCAGC
CTGGCAGCCCCAGCCACCAACCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCC
GTGGTGGTGAAGCAGGAAGCCTTGCAGCCTGAGCCCGAGCCGGTCCCCGCCCCCCAGTTG
CTTCTGGGGCCTCAGGGCCCCGGCCTCATCAAGGGGGTTGCACCTCCCACCCTCATCACC
GACTCCACAGGGACCCACCTTGTCCTCACCGTGACCAATAAGAATGCAGACAGCCCTGGC
CTGTCCAGTGGGAGCCCCCAGCAGCCCTCGTCCCAGCCTGGCTCTCCAGCGCCTGCCCCC
TCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTCTTTGGGACCCCCACTTCTCTG
CTGAAGAAGGAACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCCAAACAGCAGGAA
AATGGTTCCTCAAGCCAGCAGATGGACGACCTGTTTGACATTCTCATTCAGAGCGGAGAA
ATTTCAGCAGATTTCAAGGAGCCGCCATCCCTGCCAGGGAAGGAGAAGCCATCCCCGAAG
ACAGTCTGTGGGTCCCCCCTGGCAGCACAGCCATCACCTTCTGCTGAGCTCCCCCAGGCT
GCCCCACCTCCTCCAGGCTCACCCTCCCTCCCTGGACGCCTGGAGGACTTCCTGGAGAGC
AGCACGGGGCTGCCCCTGCTGACCAGTGGGCATGACGGGCCAGAGCCCCTTTCCCTCATT
GACGACCTCCATAGCCAGATGCTGAGCAGCACTGCCATCCTGGACCACCCCCCGTCACCC
ATGGACACCTCGGAATTGCACTTTGTTCCTGAGCCCAGCAGCACCATGGGCCTGGACCTG
GCTGATGGCCACCTGGACAGCATGGACTGGCTGGAGCTGTCGTCAGGTGGTCCCGTGCTG
AGCCTAGCCCCCCTCAGCACCACAGCCCCCAGCCTCTTCTCCACAGACTTCCTCGATGGC
CATGATTTGCAGCTGCACTGGGATTCCTGCTTG

Protein sequence predicted based on nucleotide sequence

MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLK
LKRARLADDLNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSS
DALSPEQPASHESQGSVPSPLEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQP
PLPPPPLLPPSLTNGTTIPTAKSTPTLIKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQ
YIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILNQQQQQHHNYQAILPAPPKSAGEAL
GSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKPGALPANLDDMKVAELKQELK
LRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAARLSTGPA
LVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMV
TSPLTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRM
LRQKQQLVERLKLQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPS
LAAPATNHIDPCAVAPGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPGLIKGVAPPTLIT
DSTGTHLVLTVTNKNADSPGLSSGSPQQPSSQPGSPAPAPSAQMDLEHPLQPLFGTPTSL
LKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFDILIQSGEISADFKEPPSLPGKEKPSPK
TVCGSPLAAQPSPSAELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLTSGHDGPEPLSLI
DDLHSQMLSSTAILDHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGGPVL
SLAPLSTTAPSLFSTDFLDGHDLQLHWDSCL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 25
End on reference sequence 2820
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
Mach1 T1-R
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable