DNASU Plasmid | SLC18A3 (Homo sapiens) in pJP1520 (retroviral expression vector)

Explanation of Terms

Gene:

Gene Symbol: SLC18A3

Gene Name: solute carrier family 18 (vesicular acetylcholine transporter), member 3

Original Clone ID:

FLH212824.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJP1520

Format:

CLOSED

Source:

HIP

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HsCD00074291

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1599nts

Open reading frame: 1 to 1599

Reference Sequence Alignment

ATGGAATCCGCGGAACCTGCGGGCCAGGCCCGGGCGGCGGCCACCAAGCTGTCGGAGGCT
GTGGGCGCGGCGCTGCAGGAGCCCCGGCGGCAGAGGCGCCTGGTGCTTGTTATCGTGTGC
GTGGCGCTGTTACTGGACAACATGCTGTACATGGTCATCGTGCCCATAGTGCCCGACTAC
ATCGCCCACATGCGCGGGGGCGGCGAGGGCCCCACCCGGACTCCCGAGGTGTGGGAGCCC
ACCCTGCCGCTGCCCACTCCGGCCAATGCCAGCGCCTACACGGCCAACACCTCGGCGTCC
CCGACAGCTGCGTGGCCAGCGGGCTCAGCCCTTCGGCCCCGCTACCCTACGGAGAGCGAA
GACGTGAAGATCGGGGTGCTGTTTGCTTCCAAGGCTATCCTGCAGCTGCTAGTGAACCCC
TTGAGCGGGCCCTTCATCGACCGCATGAGCTACGACGTGCCGCTGCTGATCGGCCTGGGC
GTCATGTTCGCCTCTACAGTCCTGTTCGCCTTCGCCGAGGACTACGCCACGCTGTTCGCG
GCGCGCAGCCTGCAGGGCCTGGGCTCAGCCTTCGCCGACACGTCTGGCATAGCCATGATC
GCCGATAAGTACCCGGAGGAGCCGGAGCGCAGTCGTGCACTGGGCGTGGCGCTGGCCTTC
ATTAGCTTCGGAAGCCTAGTGGCCCCGCCCTTCGGGGGCATCCTCTATGAGTTCGCCGGC
AAGCGCGTGCCCTTCTTGGTGCTAGCTGCCGTGTCGCTCTTTGACGCGCTGTTGCTGCTG
GCAGTGGCCAAACCCTTCTCGGCGGCTGCACGGGCTCGGGCCAACCTGCCAGTGGGCACT
CCCATCCACCGCCTCATGCTAGACCCCTACATTGCCGTGGTGGCCGGCGCGCTCACCACC
TGTAACATTCCCCTCGCCTTCCTCGAACCCACCATTGCCACGTGGATGAAGCATACGATG
GCGGCTTCCGAGTGGGAGATGGGCATGGCCTGGCTGCCGGCCTTCGTGCCTCATGTGCTG
GGCGTCTACCTCACCGTGCGCCTGGCGGCGCGCTACCCACACCTGCAGTGGCTGTACGGC
GCGCTTGGGCTGGCTGTGATCGGCGCCAGCTCGTGCATCGTGCCCGCCTGCCGCTCCTTC
GCGCCGCTAGTGGTCTCACTATGCGGCCTCTGTTTTGGCATAGCCCTAGTCGACACAGCA
CTGCTGCCCACGCTCGCCTTCCTGGTGGACGTGCGCCATGTCTCAGTCTATGGCAGCGTC
TACGCCATCGCCGACATCTCCTATTCGGTGGCCTACGCGCTCGGGCCCATAGTGGCAGGC
CACATTGTGCACTCGCTGGGCTTTGAGCAGCTCAGCCTTGGCATGGGACTGGCCAACCTG
CTCTATGCTCCCGTCTTGCTGCTGCTCCGCAACGTGGGCCTCCTGACGCGCTCCCGTTCC
GAGCGCGATGTGCTGCTTGATGAGCCACCGCAAGGTCTGTACGATGCGGTGCGCCTGCGT
GAGCGTCCTGTGTCTGGCCAGGACGGCGAGCCTCGCAGCCCGCCTGGCCCTTTTGATGAG
TGCGAGGACGACTACAACTACTACTACACCCGCAGCTAG

Protein sequence predicted based on nucleotide sequence

MESAEPAGQARAAATKLSEAVGAALQEPRRQRRLVLVIVCVALLLDNMLYMVIVPIVPDY
IAHMRGGGEGPTRTPEVWEPTLPLPTPANASAYTANTSASPTAAWPAGSALRPRYPTESE
DVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVLFAFAEDYATLFA
ARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAG
KRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTT
CNIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARYPHLQWLYG
ALGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSV
YAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLANLLYAPVLLLLRNVGLLTRSRS
ERDVLLDEPPQGLYDAVRLRERPVSGQDGEPRSPPGPFDECEDDYNYYYTRS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Insert was fully sequenced in parent vector; Verification by restriction enzyme digest

Reference Sequence Annotations:

End on reference sequence 1974

Start on reference sequence 376

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial    Marker: ampicillin
Host Type: bacterial    Marker: chloramphenicol
Host Type: mammalian    Marker: puromycin

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pJP1520

Synonyms:

Sequencing Primer:

Forward: JPO113

Reverse: Unknown

Description:

Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.

Comments:

One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP

Size (bp):

7894

Parent Vector:

None

Properties:

Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector

Author Name:

Ed Harlow
Joseph Pearlberg

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGCTTGCATGCCTGCAGGTCGACTCTAGGCACATAAAGAAAAACATAACTAACCAAGCTGCAGCCGAGACAGTGAAAAGAACCGTTAAAACGGTTTGTTTTAAATAAACTGAATTATTTAGAGTCATTTCTTTGGTAGGAAAGTACATTGGCACGTAAAGGAGCCCAAAGCAATCTGTGGAAAGCCCAGGCTGGGAGCCCAGCAGTTTGCATCCCCTCCTGGCGTGTACCTAAGGGTTTCTTAATTGTGTGGTTTCTAAATCTTCCAGAGGGTTTGTCTCATTCACTTCCACTTCGGTGCACAATACTTGGACGCGGATTTACTGTCTTAGCATCTATCGGTGGCCCTTCGATTGAGGCTGAACCTGAGGCCCACTTCTTCAGCTTGTTAAGGAGAGCACAAGCACCAGAAGAGGCTGACCCGGCAGACCTGTGGGCATTTTTAACAAGGGCCTCCTGGGTCTGTGGGAGGCAGGCTTACATAAGGTGCAAATTAGAAATATAAATAATAAGCCCATATCAATTTGTCATCTTTTTTTAAGCTCAAGTTTTGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGCTCAGGGCCAAGAACAGTTGGAACAGGAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTTCTCAATAAACCCTCTTGCAGTTGCATCCGACTCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGTCTTTCATTTGGAGGTTCCACCGAGATTTGGAGACCCCTGCCCAGGGACCACCGACCCCCCCGCCGGGAGGTAAGCTGGCCAGCAACTTATCTGTGTCTGTCCGATTGTCTAGTGTCTATGACTGATTTTATGCGCCTGCGTCGGTACTAGTTAGCTAACTAGCTCTGTATCTGGCGGACCCGTGGTGGAACTGACGAGTTCGGAACACCCGGCCGCAACCCTGGGAGACGTCCCAGGGACTTCGGGGGCCGTTTTTGTGGCCCGACCTGAGTCCTAAAATCCCGATCGTTTAGGACTCTTTGGTGCACCCCCCTTAGAGGAGGGATATGTGGTTCTGGTAGGAGACGAGAACCTAAAACAGTTCCCGCCTCCGTCTGAATTTTTGCTTTCGGTTTGGGACCGAAGCCGCGCCGCGCGTCTTGTCTGCTGCAGCATCGTTCTGTGTTGTCTCTGTCTGACTGTGTTTCTGTATTTGTCTGAAAATATGGGCCCGGGCTAGACTGTTACCACTCCCTTAAGTTTGACCTTAGGTCACTGGAAAGATGTCGAGCGGATCGCTCACAACCAGTCGGTAGATGTCAAGAAGAGACGTTGGGTTACCTTCTGCTCTGCAGAATGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAACCGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGCCCGCATGGACACCCAGACCAGGTCCCCTACATCGTGACCTGGGAAGCCTTGGCTTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACACCCTAAGCCTCCGCCTCCTCTTCCTCCATCCGCCCCGTCTCTCCCCCTTGAACCTCCTCGTTCGACCCCGCCTCGATCCTCCCTTTATCCAGCCCTCACTCCTTCTCTAGGCGCCCCCATATGGCCATATGAGATCTTATATGGGGCACCCCCGCCCCTTGTAAACTTCCCTGACCCTGACATGACAAGAGTTACTAACAGCCCCTCTCTCCAAGCTCACTTACAGGCTCTCTACTTAGTCCAGCACGAAGTCTGGAGACCTCTGGCGGCAGCCTACCAAGAACAACTGGACCGACCGGTGGTACCTCACCCTTACCGAGTCGGCGACACAGTGTGGGTCCGCCGACACCAGACTAAGAACCTAGAACCTCGCTGGAAAGGACCTTACACAGTCCTGCTGACCACCCCCACCGCCCTCAAAGTAGACGGCATCGCAGCTTGGATACACGCCGCCCACGTGAAGGCTGCCGACCCCGGGGGTGGACCATCCTCTAGACTGCCATGGCCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCGGCCGCCTCCGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTATTGGCCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCATGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCCCAAGCTTATAACTTCGTATAGCATACATTATACGAAGTTATAGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTCGAGGATCCGGATTAGTCCAATTTGTTAAAGACAGGATATCAGTGGTCCAGGCTCTAGTTTTGACTCAACAATATCACCAGCTGAAGCCTATAGAGTACGAGCCATAGATAAAATAAAAGATTTTATTTAGTCTCCAGAAAAAGGGGGGAATGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGCTCAGGGCCAAGAACAGTTGGAACAGGAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTTCTCAATAAACCCTCTTGCAGTTGCATCCGACTCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGTCTTTCAGTTTCTCCCACCTACACAGGTCTCACTAACATTCCTGATGTGCCGCAGGGACTCCGTCAGCCCGGTTTTTGTTTATAATAAAATGCAAGAACAGTGTTCCCTTCAAGCCAGACTACATCCTGACTCTCGGCTTTATAAAAGAATGTTGAAGGGCTCTGTGGACTATCTGCCACACGACTTTTTAAGATTTTTATGCCTCCTGGATGAGGGATTTAGTCAATCTATCCTCGTCTATTTTGCTGGCTTCTCCGTATTTTAAATTTCTAGTTTGCACTCCCTTCCTGAGAGCACGGCGATTGCAGAGTAGTTAATACTCTGAGGGCAGGCTTCTGTGAAAAGGTTGCCTGGGCTCAGTGTGAGATTTTGCCATAAAAAGGGGTCCTGCCCCTGTGTACAGACAGATCGGAATCTAGAGTGCATACTCAGAGTCCCCGCGGTTCCGGGGCTCTGATCTCAGGGCATCTTTGCCTAGAGATCCTCTACGCCGGACGCATCGTGGCCCTCGAATTAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

5659

6341

gene fragment

gag/pol/env

non-functional gag/pol/env for packaging efficiency

1871

2644

primer

JPO113

forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG

3555

3581

primer site

PBSQ

primer binding sequence (glutamine tRNA primer binding site)

1140

1156

promoter

bacterial

bacterial promoter (for chlR ORF in Creator-type inserts)

3879

4005

recombination site

LoxP

LoxP site for recombinational cloning

3651

3684

selectable marker

AmpR

ampicillin resistance gene (beta-lactamase)

6237

7094

viral LTR

5p LTR

5p viral LTR (MPSV U3, R, U5)

550

1139

viral LTR

3p LTR

3p viral LTR (MPSV U3, R, U5)

3962

4551

Gene

Gene Symbol :

SLC18A3

Symbol Nomenclature :

SLC18A3

SYNONYM :

VACHT

Designation :

solute carrier family 18 (vesicular acetylcholine), member 3|solute carrier family 18 member 3|solute carrier family 18, member 3|vesicular acetylcholine transporter

Full Nomenclature :

solute carrier family 18 (vesicular acetylcholine transporter), member 3

GENEID :

6572

HGNC :

10936

MIM :

600336

Vega :

OTTHUMG00000018196

Target GenBank:

BC007765

Reference Sequence Alignment

  

HsCD00074291      ATGGAATCCGCGGAACCTGCGGGCCAGGCCCGGGCGGCGGCCACCAAGCTGTCGGAGGCT
NM_003055.2       ATGGAATCCGCGGAACCTGCGGGCCAGGCCCGGGCGGCGGCCACCAAGCTGTCGGAGGCT
                  ************************************************************

HsCD00074291      GTGGGCGCGGCGCTGCAGGAGCCCCGGCGGCAGAGGCGCCTGGTGCTTGTTATCGTGTGC
NM_003055.2       GTGGGCGCGGCGCTGCAGGAGCCCCGGCGGCAGAGGCGCCTGGTGCTTGTTATCGTGTGC
                  ************************************************************

HsCD00074291      GTGGCGCTGTTACTGGACAACATGCTGTACATGGTCATCGTGCCCATAGTGCCCGACTAC
NM_003055.2       GTGGCGCTGTTACTGGACAACATGCTGTACATGGTCATCGTGCCCATAGTGCCCGACTAC
                  ************************************************************

HsCD00074291      ATCGCCCACATGCGCGGGGGCGGCGAGGGCCCCACCCGGACTCCCGAGGTGTGGGAGCCC
NM_003055.2       ATCGCCCACATGCGCGGGGGCGGCGAGGGCCCCACCCGGACTCCCGAGGTGTGGGAGCCC
                  ************************************************************

HsCD00074291      ACCCTGCCGCTGCCCACTCCGGCCAATGCCAGCGCCTACACGGCCAACACCTCGGCGTCC
NM_003055.2       ACCCTGCCGCTGCCCACTCCGGCCAATGCCAGCGCCTACACGGCCAACACCTCGGCGTCC
                  ************************************************************

HsCD00074291      CCGACAGCTGCGTGGCCAGCGGGCTCAGCCCTTCGGCCCCGCTACCCTACGGAGAGCGAA
NM_003055.2       CCGACAGCTGCGTGGCCAGCGGGCTCAGCCCTTCGGCCCCGCTACCCTACGGAGAGCGAA
                  ************************************************************

HsCD00074291      GACGTGAAGATCGGGGTGCTGTTTGCTTCCAAGGCTATCCTGCAGCTGCTAGTGAACCCC
NM_003055.2       GACGTGAAGATCGGGGTGCTGTTTGCTTCCAAGGCTATCCTGCAGCTGCTAGTGAACCCC
                  ************************************************************

HsCD00074291      TTGAGCGGGCCCTTCATCGACCGCATGAGCTACGACGTGCCGCTGCTGATCGGCCTGGGC
NM_003055.2       TTGAGCGGGCCCTTCATCGACCGCATGAGCTACGACGTGCCGCTGCTGATCGGCCTGGGC
                  ************************************************************

HsCD00074291      GTCATGTTCGCCTCTACAGTCCTGTTCGCCTTCGCCGAGGACTACGCCACGCTGTTCGCG
NM_003055.2       GTCATGTTCGCCTCTACAGTCCTGTTCGCCTTCGCCGAGGACTACGCCACGCTGTTCGCG
                  ************************************************************

HsCD00074291      GCGCGCAGCCTGCAGGGCCTGGGCTCAGCCTTCGCCGACACGTCTGGCATAGCCATGATC
NM_003055.2       GCGCGCAGCCTGCAGGGCCTGGGCTCAGCCTTCGCCGACACGTCTGGCATAGCCATGATC
                  ************************************************************

HsCD00074291      GCCGATAAGTACCCGGAGGAGCCGGAGCGCAGTCGTGCACTGGGCGTGGCGCTGGCCTTC
NM_003055.2       GCCGATAAGTACCCGGAGGAGCCGGAGCGCAGTCGTGCACTGGGCGTGGCGCTGGCCTTC
                  ************************************************************

HsCD00074291      ATTAGCTTCGGAAGCCTAGTGGCCCCGCCCTTCGGGGGCATCCTCTATGAGTTCGCCGGC
NM_003055.2       ATTAGCTTCGGAAGCCTAGTGGCCCCGCCCTTCGGGGGCATCCTCTATGAGTTCGCCGGC
                  ************************************************************

HsCD00074291      AAGCGCGTGCCCTTCTTGGTGCTAGCTGCCGTGTCGCTCTTTGACGCGCTGTTGCTGCTG
NM_003055.2       AAGCGCGTGCCCTTCTTGGTGCTAGCTGCCGTGTCGCTCTTTGACGCGCTGTTGCTGCTG
                  ************************************************************

HsCD00074291      GCAGTGGCCAAACCCTTCTCGGCGGCTGCACGGGCTCGGGCCAACCTGCCAGTGGGCACT
NM_003055.2       GCAGTGGCCAAACCCTTCTCGGCGGCTGCACGGGCTCGGGCCAACCTGCCAGTGGGCACT
                  ************************************************************

HsCD00074291      CCCATCCACCGCCTCATGCTAGACCCCTACATTGCCGTGGTGGCCGGCGCGCTCACCACC
NM_003055.2       CCCATCCACCGCCTCATGCTAGACCCCTACATTGCCGTGGTGGCCGGCGCGCTCACCACC
                  ************************************************************

HsCD00074291      TGTAACATTCCCCTCGCCTTCCTCGAACCCACCATTGCCACGTGGATGAAGCATACGATG
NM_003055.2       TGTAACATTCCCCTCGCCTTCCTCGAACCCACCATTGCCACGTGGATGAAGCATACGATG
                  ************************************************************

HsCD00074291      GCGGCTTCCGAGTGGGAGATGGGCATGGCCTGGCTGCCGGCCTTCGTGCCTCATGTGCTG
NM_003055.2       GCGGCTTCCGAGTGGGAGATGGGCATGGCCTGGCTGCCGGCCTTCGTGCCTCATGTGCTG
                  ************************************************************

HsCD00074291      GGCGTCTACCTCACCGTGCGCCTGGCGGCGCGCTACCCACACCTGCAGTGGCTGTACGGC
NM_003055.2       GGCGTCTACCTCACCGTGCGCCTGGCGGCGCGCTACCCACACCTGCAGTGGCTGTACGGC
                  ************************************************************

HsCD00074291      GCGCTTGGGCTGGCTGTGATCGGCGCCAGCTCGTGCATCGTGCCCGCCTGCCGCTCCTTC
NM_003055.2       GCGCTTGGGCTGGCTGTGATCGGCGCCAGCTCGTGCATCGTGCCCGCCTGCCGCTCCTTC
                  ************************************************************

HsCD00074291      GCGCCGCTAGTGGTCTCACTATGCGGCCTCTGTTTTGGCATAGCCCTAGTCGACACAGCA
NM_003055.2       GCGCCGCTAGTGGTCTCACTATGCGGCCTCTGTTTTGGCATAGCCCTAGTCGACACAGCA
                  ************************************************************

HsCD00074291      CTGCTGCCCACGCTCGCCTTCCTGGTGGACGTGCGCCATGTCTCAGTCTATGGCAGCGTC
NM_003055.2       CTGCTGCCCACGCTCGCCTTCCTGGTGGACGTGCGCCATGTCTCAGTCTATGGCAGCGTC
                  ************************************************************

HsCD00074291      TACGCCATCGCCGACATCTCCTATTCGGTGGCCTACGCGCTCGGGCCCATAGTGGCAGGC
NM_003055.2       TACGCCATCGCCGACATCTCCTATTCGGTGGCCTACGCGCTCGGGCCCATAGTGGCAGGC
                  ************************************************************

HsCD00074291      CACATTGTGCACTCGCTGGGCTTTGAGCAGCTCAGCCTTGGCATGGGACTGGCCAACCTG
NM_003055.2       CACATTGTGCACTCGCTGGGCTTTGAGCAGCTCAGCCTTGGCATGGGACTGGCCAACCTG
                  ************************************************************

HsCD00074291      CTCTATGCTCCCGTCTTGCTGCTGCTCCGCAACGTGGGCCTCCTGACGCGCTCCCGTTCC
NM_003055.2       CTCTATGCTCCCGTCTTGCTGCTGCTCCGCAACGTGGGCCTCCTGACGCGCTCCCGTTCC
                  ************************************************************

HsCD00074291      GAGCGCGATGTGCTGCTTGATGAGCCACCGCAAGGTCTGTACGATGCGGTGCGCCTGCGT
NM_003055.2       GAGCGCGATGTGCTGCTTGATGAGCCACCGCAAGGTCTGTACGATGCGGTGCGCCTGCGT
                  ************************************************************

HsCD00074291      GAGCGTCCTGTGTCTGGCCAGGACGGCGAGCCTCGCAGCCCGCCTGGCCCTTTTGATGAG
NM_003055.2       GAGCGTCCTGTGTCTGGCCAGGACGGCGAGCCTCGCAGCCCGCCTGGCCCTTTTGATGCG
                  **********************************************************.*

HsCD00074291      TGCGAGGACGACTACAACTACTACTACACCCGCAGCTAG
NM_003055.2       TGCGAGGACGACTACAACTACTACTACACCCGCAGCTAG
                  ***************************************

Pathways

Panther :

Muscarinic acetylcholine receptor 1 and 3 signaling pathway

Panther :

Muscarinic acetylcholine receptor 2 and 4 signaling pathway

Panther :

Nicotinic acetylcholine receptor signaling pathway

Reactome :

Acetylcholine Neurotransmitter Release Cycle

Reactome :

Neuronal System

Reactome :

Neurotransmitter Release Cycle

Reactome :

Transmission across Chemical Synapses

Protein

UniProt :

Q16572

HPRD :

08979

Clone

Cloning Information :

212824

HIP Master Clone ID :

59501

Original Clone ID :

FLH212824.01X

Organism

Species Specific ID:

6572

Genome

Chromosome :

Map Location :

10q11.2

Ensembl :

ENSG00000187714

Reagents

Labome :

vesicular-acetylcholine-transporter-antibody