DNASU Plasmid | CART1 (Homo sapiens) in pJP1520 (retroviral expression vector)

Explanation of Terms

Gene:

Gene Symbol: ALX1

Gene Name: ALX homeobox 1

Original Clone ID:

FLH212852.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJP1520

Format:

CLOSED

Source:

HIP

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HsCD00074313

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 981nts

Open reading frame: 1 to 981

Reference Sequence Alignment

ATGGAGTTTCTGAGCGAGAAGTTTGCCCTCAAGAGCCCTCCGAGTAAAAACAGTGACTTT
TACATGGGCGCAGGAGGTCCTCTGGAGCACGTTATGGAGACGCTGGACAATGAGTCCTTT
TACAGCAAAGCGTCTGCAGGCAAATGCGTGCAGGCCTTCGGACCCCTGCCCCGCGCCGAG
CATCACGTGCGCTTGGAGAGGACCTCGCCCTGTCAGGACAGCAGCGTGAACTATGGGATC
ACTAAAGTAGAAGGACAGCCCCTTCACACCGAACTGAATAGAGCTATGGACAACTGTAAC
AGTCTCCGAATGTCTCCCGTGAAAGGGATGCAAGAGAAGGGAGAGCTGGATGAACTTGGG
GATAAATGTGATAGCAATGTATCCAGCAGTAAGAAACGGAGGCACCGAACCACCTTCACC
AGTTTGCAGCTAGAGGAGCTGGAGAAAGTCTTTCAGAAAACTCATTACCCGGATGTGTAT
GTCAGAGAACAGCTTGCTCTGAGGACAGAGCTCACTGAGGCCAGGGTCCAGGTTTGGTTT
CAAAATCGAAGGGCCAAATGGAGAAAAAGGGAACGTTATGGCCAAATACAACAAGCGAAA
AGCCATTTTGCTGCCACCTATGATATATCAGTTTTGCCAAGGACTGACAGCTACCCACAG
ATTCAGAACAATTTGTGGGCAGGAAATGCAAGTGGTGGTTCTGTGGTTACTTCATGCATG
TTACCACGTGACACTTCCTCCTGTATGACACCTTATTCTCACTCGCCTCGGACAGATTCC
AGTTACACGGGGTTTTCAAACCACCAGAACCAGTTCAGCCACGTGCCCCTCAACAATTTT
TTCACTGACTCTCTTCTTACTGGGGCAACCAATGGACATGCATTTGAAACAAAGCCAGAG
TTTGAAAGGAGGTCTTCCAGTATCGCAGTTCTTCGAATGAAAGCCAAGGAGCACACCGCC
AATATTTCATGGGCCATGTAG

Protein sequence predicted based on nucleotide sequence

MEFLSEKFALKSPPSKNSDFYMGAGGPLEHVMETLDNESFYSKASAGKCVQAFGPLPRAE
HHVRLERTSPCQDSSVNYGITKVEGQPLHTELNRAMDNCNSLRMSPVKGMQEKGELDELG
DKCDSNVSSSKKRRHRTTFTSLQLEELEKVFQKTHYPDVYVREQLALRTELTEARVQVWF
QNRRAKWRKRERYGQIQQAKSHFAATYDISVLPRTDSYPQIQNNLWAGNASGGSVVTSCM
LPRDTSSCMTPYSHSPRTDSSYTGFSNHQNQFSHVPLNNFFTDSLLTGATNGHAFETKPE
FERRSSSIAVLRMKAKEHTANISWAM*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Insert was fully sequenced in parent vector; Verification by restriction enzyme digest

Reference Sequence Annotations:

Start on reference sequence 11

End on reference sequence 991

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial    Marker: ampicillin
Host Type: bacterial    Marker: chloramphenicol
Host Type: mammalian    Marker: puromycin

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pJP1520

Synonyms:

Sequencing Primer:

Forward: JPO113

Reverse: Unknown

Description:

Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.

Comments:

One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP

Size (bp):

7894

Parent Vector:

None

Properties:

Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector

Author Name:

Ed Harlow
Joseph Pearlberg

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGCTTGCATGCCTGCAGGTCGACTCTAGGCACATAAAGAAAAACATAACTAACCAAGCTGCAGCCGAGACAGTGAAAAGAACCGTTAAAACGGTTTGTTTTAAATAAACTGAATTATTTAGAGTCATTTCTTTGGTAGGAAAGTACATTGGCACGTAAAGGAGCCCAAAGCAATCTGTGGAAAGCCCAGGCTGGGAGCCCAGCAGTTTGCATCCCCTCCTGGCGTGTACCTAAGGGTTTCTTAATTGTGTGGTTTCTAAATCTTCCAGAGGGTTTGTCTCATTCACTTCCACTTCGGTGCACAATACTTGGACGCGGATTTACTGTCTTAGCATCTATCGGTGGCCCTTCGATTGAGGCTGAACCTGAGGCCCACTTCTTCAGCTTGTTAAGGAGAGCACAAGCACCAGAAGAGGCTGACCCGGCAGACCTGTGGGCATTTTTAACAAGGGCCTCCTGGGTCTGTGGGAGGCAGGCTTACATAAGGTGCAAATTAGAAATATAAATAATAAGCCCATATCAATTTGTCATCTTTTTTTAAGCTCAAGTTTTGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGCTCAGGGCCAAGAACAGTTGGAACAGGAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTTCTCAATAAACCCTCTTGCAGTTGCATCCGACTCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGTCTTTCATTTGGAGGTTCCACCGAGATTTGGAGACCCCTGCCCAGGGACCACCGACCCCCCCGCCGGGAGGTAAGCTGGCCAGCAACTTATCTGTGTCTGTCCGATTGTCTAGTGTCTATGACTGATTTTATGCGCCTGCGTCGGTACTAGTTAGCTAACTAGCTCTGTATCTGGCGGACCCGTGGTGGAACTGACGAGTTCGGAACACCCGGCCGCAACCCTGGGAGACGTCCCAGGGACTTCGGGGGCCGTTTTTGTGGCCCGACCTGAGTCCTAAAATCCCGATCGTTTAGGACTCTTTGGTGCACCCCCCTTAGAGGAGGGATATGTGGTTCTGGTAGGAGACGAGAACCTAAAACAGTTCCCGCCTCCGTCTGAATTTTTGCTTTCGGTTTGGGACCGAAGCCGCGCCGCGCGTCTTGTCTGCTGCAGCATCGTTCTGTGTTGTCTCTGTCTGACTGTGTTTCTGTATTTGTCTGAAAATATGGGCCCGGGCTAGACTGTTACCACTCCCTTAAGTTTGACCTTAGGTCACTGGAAAGATGTCGAGCGGATCGCTCACAACCAGTCGGTAGATGTCAAGAAGAGACGTTGGGTTACCTTCTGCTCTGCAGAATGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAACCGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGCCCGCATGGACACCCAGACCAGGTCCCCTACATCGTGACCTGGGAAGCCTTGGCTTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACACCCTAAGCCTCCGCCTCCTCTTCCTCCATCCGCCCCGTCTCTCCCCCTTGAACCTCCTCGTTCGACCCCGCCTCGATCCTCCCTTTATCCAGCCCTCACTCCTTCTCTAGGCGCCCCCATATGGCCATATGAGATCTTATATGGGGCACCCCCGCCCCTTGTAAACTTCCCTGACCCTGACATGACAAGAGTTACTAACAGCCCCTCTCTCCAAGCTCACTTACAGGCTCTCTACTTAGTCCAGCACGAAGTCTGGAGACCTCTGGCGGCAGCCTACCAAGAACAACTGGACCGACCGGTGGTACCTCACCCTTACCGAGTCGGCGACACAGTGTGGGTCCGCCGACACCAGACTAAGAACCTAGAACCTCGCTGGAAAGGACCTTACACAGTCCTGCTGACCACCCCCACCGCCCTCAAAGTAGACGGCATCGCAGCTTGGATACACGCCGCCCACGTGAAGGCTGCCGACCCCGGGGGTGGACCATCCTCTAGACTGCCATGGCCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCGGCCGCCTCCGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTATTGGCCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCATGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCCCAAGCTTATAACTTCGTATAGCATACATTATACGAAGTTATAGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTCGAGGATCCGGATTAGTCCAATTTGTTAAAGACAGGATATCAGTGGTCCAGGCTCTAGTTTTGACTCAACAATATCACCAGCTGAAGCCTATAGAGTACGAGCCATAGATAAAATAAAAGATTTTATTTAGTCTCCAGAAAAAGGGGGGAATGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGCTCAGGGCCAAGAACAGTTGGAACAGGAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTTCTCAATAAACCCTCTTGCAGTTGCATCCGACTCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGTCTTTCAGTTTCTCCCACCTACACAGGTCTCACTAACATTCCTGATGTGCCGCAGGGACTCCGTCAGCCCGGTTTTTGTTTATAATAAAATGCAAGAACAGTGTTCCCTTCAAGCCAGACTACATCCTGACTCTCGGCTTTATAAAAGAATGTTGAAGGGCTCTGTGGACTATCTGCCACACGACTTTTTAAGATTTTTATGCCTCCTGGATGAGGGATTTAGTCAATCTATCCTCGTCTATTTTGCTGGCTTCTCCGTATTTTAAATTTCTAGTTTGCACTCCCTTCCTGAGAGCACGGCGATTGCAGAGTAGTTAATACTCTGAGGGCAGGCTTCTGTGAAAAGGTTGCCTGGGCTCAGTGTGAGATTTTGCCATAAAAAGGGGTCCTGCCCCTGTGTACAGACAGATCGGAATCTAGAGTGCATACTCAGAGTCCCCGCGGTTCCGGGGCTCTGATCTCAGGGCATCTTTGCCTAGAGATCCTCTACGCCGGACGCATCGTGGCCCTCGAATTAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

5659

6341

gene fragment

gag/pol/env

non-functional gag/pol/env for packaging efficiency

1871

2644

primer

JPO113

forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG

3555

3581

primer site

PBSQ

primer binding sequence (glutamine tRNA primer binding site)

1140

1156

promoter

bacterial

bacterial promoter (for chlR ORF in Creator-type inserts)

3879

4005

recombination site

LoxP

LoxP site for recombinational cloning

3651

3684

selectable marker

AmpR

ampicillin resistance gene (beta-lactamase)

6237

7094

viral LTR

5p LTR

5p viral LTR (MPSV U3, R, U5)

550

1139

viral LTR

3p LTR

3p viral LTR (MPSV U3, R, U5)

3962

4551

Gene

Gene Symbol :

ALX1

Symbol Nomenclature :

ALX1

SYNONYM :

CART1; FND3; HEL23

Designation :

ALX homeobox protein 1|CART-1|cartilage paired-class homeoprotein 1|epididymis luminal protein 23

Full Nomenclature :

ALX homeobox 1

GENEID :

8092

HGNC :

1494

MIM :

601527

Vega :

OTTHUMG00000169820

Target GenBank:

BC010923

Reference Sequence Alignment

  

HsCD00074313      ATGGAGTTTCTGAGCGAGAAGTTTGCCCTCAAGAGCCCTCCGAGTAAAAACAGTGACTTT
NM_006982.2       ATGGAGTTTCTGAGCGAGAAGTTTGCCCTCAAGAGCCCTCCGAGTAAAAACAGTGACTTT
                  ************************************************************

HsCD00074313      TACATGGGCGCAGGAGGTCCTCTGGAGCACGTTATGGAGACGCTGGACAATGAGTCCTTT
NM_006982.2       TACATGGGCGCAGGAGGTCCTCTGGAGCACGTTATGGAGACGCTGGACAATGAGTCCTTT
                  ************************************************************

HsCD00074313      TACAGCAAAGCGTCTGCAGGCAAATGCGTGCAGGCCTTCGGACCCCTGCCCCGCGCCGAG
NM_006982.2       TACAGCAAAGCGTCTGCAGGCAAATGCGTGCAGGCCTTCGGACCCCTGCCCCGCGCCGAG
                  ************************************************************

HsCD00074313      CATCACGTGCGCTTGGAGAGGACCTCGCCCTGTCAGGACAGCAGCGTGAACTATGGGATC
NM_006982.2       CATCACGTGCGCTTGGAGAGGACCTCGCCCTGTCAGGACAGCAGCGTGAACTATGGGATC
                  ************************************************************

HsCD00074313      ACTAAAGTAGAAGGACAGCCCCTTCACACCGAACTGAATAGAGCTATGGACAACTGTAAC
NM_006982.2       ACTAAAGTAGAAGGACAGCCCCTTCACACCGAACTGAATAGAGCTATGGACAACTGTAAC
                  ************************************************************

HsCD00074313      AGTCTCCGAATGTCTCCCGTGAAAGGGATGCAAGAGAAGGGAGAGCTGGATGAACTTGGG
NM_006982.2       AGTCTCCGAATGTCTCCCGTGAAAGGGATGCAAGAGAAGGGAGAGCTGGATGAACTTGGG
                  ************************************************************

HsCD00074313      GATAAATGTGATAGCAATGTATCCAGCAGTAAGAAACGGAGGCACCGAACCACCTTCACC
NM_006982.2       GATAAATGTGATAGCAATGTATCCAGCAGTAAGAAACGGAGGCACCGAACCACCTTCACC
                  ************************************************************

HsCD00074313      AGTTTGCAGCTAGAGGAGCTGGAGAAAGTCTTTCAGAAAACTCATTACCCGGATGTGTAT
NM_006982.2       AGTTTGCAGCTAGAGGAGCTGGAGAAAGTCTTTCAGAAAACTCATTACCCGGATGTGTAT
                  ************************************************************

HsCD00074313      GTCAGAGAACAGCTTGCTCTGAGGACAGAGCTCACTGAGGCCAGGGTCCAGGTTTGGTTT
NM_006982.2       GTCAGAGAACAGCTTGCTCTGAGGACAGAGCTCACTGAGGCCAGGGTCCAGGTTTGGTTT
                  ************************************************************

HsCD00074313      CAAAATCGAAGGGCCAAATGGAGAAAAAGGGAACGTTATGGCCAAATACAACAAGCGAAA
NM_006982.2       CAAAATCGAAGGGCCAAATGGAGAAAAAGGGAACGTTATGGCCAAATACAACAAGCGAAA
                  ************************************************************

HsCD00074313      AGCCATTTTGCTGCCACCTATGATATATCAGTTTTGCCAAGGACTGACAGCTACCCACAG
NM_006982.2       AGCCATTTTGCTGCCACCTATGATATATCAGTTTTGCCAAGGACTGACAGCTACCCACAG
                  ************************************************************

HsCD00074313      ATTCAGAACAATTTGTGGGCAGGAAATGCAAGTGGTGGTTCTGTGGTTACTTCATGCATG
NM_006982.2       ATTCAGAACAATTTGTGGGCAGGAAATGCAAGTGGTGGTTCTGTGGTTACTTCATGCATG
                  ************************************************************

HsCD00074313      TTACCACGTGACACTTCCTCCTGTATGACACCTTATTCTCACTCGCCTCGGACAGATTCC
NM_006982.2       TTACCACGTGACACTTCCTCCTGTATGACACCTTATTCTCACTCGCCTCGGACAGATTCC
                  ************************************************************

HsCD00074313      AGTTACACGGGGTTTTCAAACCACCAGAACCAGTTCAGCCACGTGCCCCTCAACAATTTT
NM_006982.2       AGTTACACGGGGTTTTCAAACCACCAGAACCAGTTCAGCCACGTGCCCCTCAACAATTTT
                  ************************************************************

HsCD00074313      TTCACTGACTCTCTTCTTACTGGGGCAACCAATGGACATGCATTTGAAACAAAGCCAGAG
NM_006982.2       TTCACTGACTCTCTTCTTACTGGGGCAACCAATGGACATGCATTTGAAACAAAGCCAGAG
                  ************************************************************

HsCD00074313      TTTGAAAGGAGGTCTTCCAGTATCGCAGTTCTTCGAATGAAAGCCAAGGAGCACACCGCC
NM_006982.2       TTTGAAAGGAGGTCTTCCAGTATCGCAGTTCTTCGAATGAAAGCCAAGGAGCACACCGCC
                  ************************************************************

HsCD00074313      AATATTTCATGGGCCATGTAG
NM_006982.2       AATATTTCATGGGCCATGTAA
                  ********************.