Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
plate source, forward and reverse reads, failed to contig with refseq. BLAST returns HsCD00821095 (AATK gene)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745290
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4818nts
Open reading frame: 1 to 4818
ATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCC
CGTCTTCGTCAGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTC
TCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATT
GAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGA
ATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGA
CCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCAC
AATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTC
ATGGTTGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAG
CCCACCATTTCATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACT
ATACGAGGAGGAAAGCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTT
TGTGTTGGTACCAATATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTA
GAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCA
GAATTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGAT
GGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAG
GTGACAGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCT
GAAGCATCTGCTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAA
CCCCGTGACCAGGTTGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGA
AATCCTCAACCAGCTATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATAT
CAACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACT
AATGTCCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGC
ATCATCACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTT
ATTCGACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGT
GTGGCCACAGGCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCA
ACCCAAGACTCTCGAATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAG
CTGGGTGATACTGGTCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGG
AGTGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGAC
CCAAATTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACA
GTCACATTATCATGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATA
GAAGCCTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACA
GAAACATCTGCCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCA
GCTAATGCATATGGAATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGAT
GTCCTACCAACAAGTCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCT
GTTCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACA
GTAGATCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCC
AACCACGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTG
GTAATCCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAAT
GAATTTCAAGGAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGT
GCCCCACCCCAAGGTGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTT
AGTTGGCAGCCACCTCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGG
TGTCTGGGCAATGAAACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCC
GTGGTCATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACT
GGGGCTGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAAC
CCTGTGTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAG
CCGGCCTTCATAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATC
TGGCTTTATCGACACCGCAAGAAGAGAAACGGACTTACTAGTACATACGCGGGTATCAGA
AAAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTC
AACATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAAC
AACCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAAC
CTCACTACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAAC
AAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAAC
AAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAAT
CCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAAC
AACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAG
AAACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGAT
TATCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATCATACGACCAGAAC
ACAGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCAC
AAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAACTGGGCAGACCTG
CTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAACATTTCT
GTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTG
CAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGA
GCAGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCC
TCCCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGACTGGCCACATG
CAGCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATC
TCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGATATGGATACGGAT
GCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAACCAGAAGG
CTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCT
GTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGC
GGACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAG
GCAGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGAT
GCTGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCT
ACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGCCAAGAAACTGAAA
CACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACCTCCTGTG
CCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCT
GTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAA
GGAAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACA
AATCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAAC
AAGGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTA
CCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCCCAGTTCCTCAAGC
TCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGGTCGAAGA
AATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAA
TTAGAGGAAACTGAAAGC
Protein sequence predicted based on nucleotide sequence
MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTI EWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSH NASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERIT IRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSA EFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKA EASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSY QPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPV IRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAK LGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNT VTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRA ANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWT VDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFN EFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVW CLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGN PVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIR KVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSN LTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVN PSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKD YRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADL LPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRG AASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPI SPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESS VTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQD AAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPV PPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRT NPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSS SMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
