Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745863
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6267nts
Open reading frame: 1 to 6267
ATGGAGGACACCCAGGCCATTGACTGGGACGTTGAGGAGGAGGAGGAGACAGAGCAATCC
AGTGAATCGTTGAGATGTAACGTGGAGCCCGTAGGGCGGCTACATATCTTTAGTGGTGCT
CATGGACCAGAAAAAGATTTCCCACTACACCTCGGGAAGAATGTGGTCGGCCGAATGCCT
GACTGCTCTGTGGCCCTGCCATTTCCATCTATCTCTAAACAACATGCCGAGATCGAAATC
TTAGCCTGGGATAAGGCACCTATTCTCCGAGACTGTGGGTCCCTTAATGGTACTCAAATC
CTGCGACCTCCCAAGGTTCTGAGTCCCGGGGTGAGTCACCGTCTCAGGGACCAGGAGTTA
ATTCTGTTTGCCGACTTGCTCTGCCAGTACCATCGCCTGGACGTTTCCCTGCCTTTTGTC
TCCCGCGGCCCTCTGACAGTCGAAGAGACACCTAGAGTACAGGGCGAAACTCAACCACAG
AGGCTTTTGTTGGCTGAGGACTCGGAGGAGGAAGTAGATTTTCTTTCTGAAAGGCGTATG
GTAAAAAAATCCAGGACCACATCTTCATCCGTGATAGTGCCAGAGAGCGATGAAGAGGGG
CATTCCCCGGTCCTCGGAGGCCTCGGACCGCCTTTTGCCTTCAATTTGAACAGCGATACA
GATGTCGAAGAAGGTCAGCAACCAGCTACAGAGGAGGCATCCTCCGCCGCCAGAAGAGGT
GCGACTGTAGAGGCAAAGCAGTCTGAGGCTGAGGTTGTAACTGAAATCCAGCTTGAAAAG
GATCAGCCTTTAGTGAAGGAGAGGGATAATGATACAAAAGTCAAGCGGGGTGCAGGGAAT
GGGGTGGTTCCAGCTGGGGTGATTTTAGAGAGGTCCCAACCTCCTGGAGAGGATAGCGAC
ACCGATGTCGATGATGACTCAAGGCCACCTGGAAGGCCCGCCGAGGTCCATTTGGAGCGG
GCTCAGCCGTTTGGCTTCATCGACTCCGACACTGATGCGGAAGAAGAGAGGATCCCCGCA
ACCCCAGTTGTCATTCCTATGAAGAAGCGCAAGATCTTCCACGGAGTGGGTACACGCGGA
CCTGGAGCACCAGGACTGGCCCATTTGCAGGAGTCCCAGGCCGGTAGTGATACCGATGTG
GAAGAAGGCAAGGCCCCACAGGCCGTCCCTCTGGAGAAATCCCAAGCTTCCATGGTTATT
AACAGCGACACAGATGACGAAGAAGAAGTCTCAGCAGCCCTGACTTTGGCACACCTGAAA
GAGAGTCAGCCTGCTATCTGGAACAGGGATGCCGAAGAGGACATGCCCCAACGTGTCGTC
CTTCTGCAGCGCAGCCAAACAACCACTGAGAGAGACAGTGACACCGACGTCGAGGAGGAA
GAGCTCCCCGTGGAAAATAGAGAAGCTGTGCTCAAGGATCACACAAAGATTAGGGCCCTT
GTTAGAGCACATTCAGAGAAGGACCAACCCCCTTTTGGGGACAGTGATGACAGTGTGGAA
GCCGATAAGAGCTCACCTGGGATCCACTTGGAAAGAAGCCAAGCCTCCACCACAGTGGAC
ATCAACACACAAGTTGAGAAGGAAGTCCCGCCAGGGTCAGCCATTATACATATAAAGAAA
CATCAGGTTTCTGTTGAGGGGACAAATCAAACTGATGTGAAAGCCGTTGGGGGACCAGCA
AAGCTGCTTGTGGTATCCCTAGAGGAAGCCTGGCCTCTGCATGGGGACTGTGAAACAGAT
GCCGAGGAGGGCACCTCTCTGACTGCATCAGTAGTTGCAGATGTAAGAAAGAGTCAGTTG
CCAGCCGAAGGGGATGCTGGGGCCGAGTGGGCTGCAGCTGTTCTTAAGCAGGAGAGAGCC
CATGAGGTTGGCGCCCAGGGTGGGCCACCCGTGGCACAAGTGGAGCAGGACCTCCCTATC
TCAAGAGAGAACCTGACAGATCTGGTGGTGGACACAGACACTCTAGGGGAATCCACCCAG
CCACAGCGAGAGGGAGCCCAGGTCCCGACAGGAAGGGAGAGAGAGCAACATGTGGGTGGG
ACCAAGGACTCCGAAGACAACTATGGTGATTCTGAGGATCTCGACCTACAAGCTACCCAG
TGCTTTTTGGAGAATCAGGGCCTGGAGGCAGTCCAGAGTATGGAGGATGAACCTACACAG
GCCTTCATGTTGACTCCACCTCAAGAGCTTGGCCCTTCCCATTGCAGCTTCCAGACAACA
GGTACACTAGATGAACCATGGGAGGTTCTCGCTACTCAGCCCTTCTGTCTTCGCGAGTCT
GAAGATTCTGAAACCCAGCCTTTTGACACGCACCTTGAGGCCTATGGACCTTGCTTGTCC
CCACCTAGGGCAATACCAGGAGATCAACATCCAGAGAGTCCAGTTCACACAGAGCCAATG
GGGATCCAGGGCAGAGGGAGGCAGACCGTGGATAAAGTGATGGGTATACCAAAAGAGACA
GCAGAGAGAGTGGGACCTGAGCGAGGGCCACTGGAAAGAGAAACCGAGAAACTGCTACCC
GAAAGGCAGACAGATGTGACAGGAGAAGAAGAATTGACCAAGGGGAAACAGGACCGAGAG
CAAAAGCAGTTGCTAGCTAGAGACACGCAGAGACAAGAATCTGACAAAAATGGGGAAAGT
GCAAGTCCTGAAAGGGATAGGGAAAGTTTGAAGGTAGAAATTGAAACCTCTGAGGAAATA
CAAGAAAAACAAGTACAGAAGCAGACCCTTCCAAGCAAAGCATTTGAGAGGGAAGTCGAG
CGACCAGTAGCCAACAGAGAGTGCGATCCAGCCGAGTTAGAAGAGAAGGTGCCTAAAGTC
ATCCTCGAGAGAGATACACAGAGGGGCGAGCCTGAGGGAGGGAGTCAGGACCAAAAAGGG
CAGGCCTCCAGCCCAACTCCAGAACCTGGGGTGGGCGCGGGGGACCTTCCGGGACCTACC
TCAGCTCCCGTGCCTTCTGGCAGCCAGTCAGGTGGCAGGGGATCTCCAGTGAGCCCCAGG
AGACATCAGAAAGGCCTCCTGAATTGCAAGATGCCACCCGCTGAAAAGGCTTCCAGGATC
AGAGCAGCTGAGAAGGTTTCCAGAGGCGATCAGGAATCCCCCGATGCTTGTCTGCCCCCT
ACAGTACCTGAAGCCCCAGCCCCACCGCAAAAGCCCCTTAACTCTCAGAGCCAGAAGCAT
CTGGCACCTCCCCCCCTTCTTTCTCCCTTGTTGCCTTCCATCAAGCCAACCGTTCGTAAG
ACAAGGCAAGATGGGTCCCAGGAAGCTCCCGAGGCTCCCCTGTCTTCAGAGTTGGAGCCT
TTCCACCCAAAGCCTAAAATTAGAACTCGGAAGTCCTCCAGAATGACACCCTTTCCAGCT
ACCTCTGCAGCACCTGAACCTCACCCTTCAACCTCCACAGCCCAGCCAGTTACTCCTAAG
CCGACCTCTCAGGCCACTAGGTCTAGGACAAATAGGTCCTCCGTCAAAACCCCTGAACCC
GTGGTCCCTACAGCCCCTGAATTACAGCCCTCAACCTCTACCGACCAGCCTGTGACATCT
GAACCCACGTCTCAAGTTACTAGGGGACGCAAAAGTAGATCTTCTGTTAAGACCCCGGAA
ACAGTTGTCCCAACAGCCCTTGAGCTCCAACCTAGCACATCCACGGACCGACCTGTGACC
TCTGAACCGACCTCGCAGGCTACCCGCGGAAGGAAAAATCGATCCTCCGTTAAGACTCCA
GAGCCTGTGGTGCCTACCGCACCCGAACTCCAGCCTTCAACATCTACCGATCAGCCCGTG
ACTTCGGAGCCTACCTATCAGGCTACTAGAGGCAGAAAAAATCGCTCCTCCGTGAAGACT
CCCGAACCAGTGGTGCCCACAGCACCAGAGCTCCGGCCTTCTACCTCCACCGACCGACCG
GTCACACCCAAGCCCACCTCTCGGACCACTAGGAGCAGAACAAATATGTCCTCGGTGAAG
ACGCCTGAAACTGTTGTTCCTACCGCCCCAGAACTGCAGATTTCCACCAGCACCGACCAA
CCTGTTACCCCTAAGCCCACTTCTCGCACCACAAGGAGTAGGACTAATATGTCTTCCGTC
AAGAACCCTGAATCAACCGTCCCTATCGCCCCTGAGTTACCACCCTCTACCTCTACTGAG
CAGCCTGTCACCCCCGAGCCCACATCTAGGGCTACTCGGGGACGAAAAAATAGATCCTCT
GGCAAGACACCTGAGACACTCGTCCCCACAGCGCCTAAGCTGGAGCCCTCCACTTCCACT
GACCAGCCGGTCACTCCTGAGCCAACATCACAGGCCACCAGAGGCCGGACCAATAGGTCT
TCAGTTAAGACACCGGAGACAGTCGTTCCCACAGCTCCTGAGTTGCAGCCATCCACCTCG
ACAGACCAGCCAGTGACGCCAGAGCCCACGTCGCAGGCCACAAGGGGCAGAACAGATAGA
TCCAGTGTGAAGACCCCAGAAACAGTGGTCCCCACTGCCCCAGAGCTACAGGCTTCTGCT
TCCACCGATCAACCTGTCACTTCTGAGCCGACATCTCGGACAACCAGGGGAAGAAAGAAT
CGGTCTTCTGTCAAGACTCCTGAAACCGTTGTGCCTGCCGCTCCTGAACTACAGCCTTCG
ACCTCGACTGACCAACCGGTTACCCCGGAGCCAACCAGTCGGGCAACTAGGGGCAGGACA
AACAGATCCTCGGTCAAGACGCCCGAATCAATTGTCCCTATAGCGCCTGAGCTTCAGCCT
TCCACCTCCAGGAACCAGCTCGTCACCCCTGAGCCCACCAGTCGCGCCACCAGGTGCAGA
ACCAACCGCTCCTCAGTGAAGACACCCGAGCCAGTTGTGCCCACCGCCCCCGAACCCCAT
CCTACCACCTCTACGGACCAGCCCGTCACACCTAAGCTCACATCTCGCGCTACTAGGAGA
AAAACCAACAGGTCCTCTGTCAAAACTCCCAAACCAGTTGAACCCGCAGCTTCTGATCTT
GAGCCTTTTACTCCCACAGACCAATCCGTCACTCCGGAGGCCATAGCTCAGGGTGGTCAG
AGTAAAACACTGAGGTCATCCACAGTAAGAGCTATGCCGGTTCCTACTACCCCCGAATTC
CAATCTCCCGTCACCACAGATCAGCCAATTTCACCTGAGCCTATTACTCAACCCAGTTGC
ATCAAGAGGCAGCGAGCCGCTGGGAACCCCGGCTCCCTGGCAGCTCCTATTGACCATAAG
CCTTGCTCCGCCCCCTTGGAACCTAAATCGCAGGCGTCAAGGAACCAAAGATGGGGAGCA
GTGAGAGCAGCCGAATCCCTTACAGCCATTCCTGAACCGGCTTCTCCCCAACTTCTTGAG
ACACCAATTCATGCCTCCCAGATCCAAAAGGTTGAGCCAGCAGGTAGATCTAGGTTCACA
CCGGAATTGCAGCCTAAGGCTTCTCAAAGCCGCAAGAGGTCTTTAGCTACCATGGATTCA
CCACCACATCAAAAACAGCCTCAAAGAGGCGAAGTCTCCCAGAAAACAGTGATTATCAAA
GAAGAGGAAGAAGATACTGCAGAGAAGCCAGGGAAGGAAGAGGATGTCGTTACTCCAAAA
CCAGGCAAGAGAAAGCGCGACCAGGCAGAGGAGGAGCCCAACAGAATACCAAGCCGCAGC
CTGCGCCGGACAAAACTTAACCAAGAATCAACAGCTCCCAAAGTGCTCTTCACAGGAGTG
GTGGATGCTCGGGGAGAACGGGCTGTGCTGGCACTCGGCGGAAGTCTCGCTGGTTCAGCG
GCAGAAGCTTCCCACCTGGTCACTGATCGCATCCGCCGGACCGTCAAGTTCCTGTGTGCC
CTCGGGCGGGGAATTCCTATTCTGTCCCTGGACTGGCTCCATCAGTCCCGCAAGGCTGGT
TTCTTCTTACCCCCGGATGAATATGTGGTCACCGACCCTGAACAGGAAAAGAACTTCGGC
TTTAGCCTTCAGGACGCCCTGAGCAGGGCCCGGGAGCGGAGGCTGCTGGAGGGATATGAG
ATCTATGTGACCCCTGGAGTCCAGCCCCCACCACCTCAGATGGGAGAGATTATTAGCTGC
TGTGGCGGCACATACCTACCCAGCATGCCTAGGTCCTATAAGCCCCAAAGAGTTGTGATC
ACTTGCCCTCAGGATTTCCCTCATTGCTCTATTCCACTGCGGGTCGGGCTGCCACTCCTC
TCGCCTGAGTTCCTGCTGACTGGAGTGCTGAAGCAAGAAGCTAAGCCCGAGGCATTTGTG
CTTTCCCCTCTAGAGATGTCATCTACC
Protein sequence predicted based on nucleotide sequence
MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMP DCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQEL ILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRM VKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRG ATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSD TDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRG PGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLK ESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRAL VRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKK HQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQL PAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQ PQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQ AFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLS PPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLP ERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEI QEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKG QASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRI RAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRK TRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPK PTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPE TVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPV TSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVK TPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTE QPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRS SVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASA STDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRT NRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPH PTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQ SKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHK PCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFT PELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPK PGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSA AEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFG FSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVI TCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
