Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746261
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5547nts
Open reading frame: 1 to 5547
ATGCCGCGCAGCCCAACATCCAGCGAGGACGAAATGGCCCAGAGCTTCTCCGACTACTCC
GTGGGGTCGGAGTCCGACAGCTCCAAAGAAGAGACAATCTATGACACGATCCGGGCCACT
GCCGAGAAGCCGGGCGGCGCCAGGACGGAGGAGTCCCAGGGCAATACGCTGGTGATCCGC
GTGGTGATTCATGACCTGCAGCAAACGAAATGTATTCGATTTAACCCGGATGCTACAGTG
TGGGTTGCAAAGCAGCGGATCCTGTGTACATTAACCCAAAGCTTGAAAGATGTGCTGAAC
TACGGCCTGTTCCAACCGGCCAGCAATGGGCGTGACGGCAAGTTCCTGGACGAGGAGCGC
CTCCTTCGCGAGTACCCACAGCCTGTGGGTGAGGGCGTTCCCTCACTGGAGTTTAGATAC
AAGAAAAGGGTGTATAAACAAGCCAGTCTCGATGAGAAACAGTTGGCCAAGCTGCACACG
AAGACCAATCTGAAGAAATGCATGGATCACATTCAGCATCGCTTGGTGGAGAAGATCACC
AAGATGCTGGACCGAGGCCTTGATCCCAATTTCCACGACCCGGAGACAGGCGAGACACCC
CTGACCTTAGCCGCTCAGCTTGACGACTCTGTGGAGGTGATCAAAGCACTGAAAAATGGT
GGCGCTCACCTGGACTTCCGTGCTAAAGATGGGATGACCGCCCTACATAAAGCTGCCAGA
GCGAGGAACCAAGTTGCCCTGAAGACACTTTTAGAGCTTGGCGCATCCCCCGATTATAAA
GACAGTTACGGGCTCACCCCGCTGTATCACACAGCTATCGTCGGAGGAGATCCCTACTGC
TGCGAACTGCTCCTGCACGAACACGCCACTGTCTGCTGTAAAGATGAGAACGGCTGGCAC
GAGATCCACCAGGCTTGCAGGTACGGCCACGTGCAGCACCTGGAGCACCTCCTGTTCTAC
GGCGCAGACATGAGTGCCCAGAATGCCTCGGGCAACACAGCTTTGCACATCTGCGCCCTC
TACAACCAGGACAGCTGTGCAAGAGTGCTTCTGTTTCGAGGCGGAAATAAGGAGTTGAAA
AACTACAACAGCCAGACTCCATTTCAGGTGGCCATAATAGCAGGCAACTTTGAGCTGGCA
GAATACATCAAGAACCACAAGGAAACAGACATTGTCCCCTTCCGAGAGGCCCCGGCGTAC
TCCAACCGACGGCGGCGGCCCCCTAACACGCTGGCCGCTCCGCGGGTCCTGTTGCGCTCT
AACAGCGACAACAACCTGAACGCCAGCGCTCCCGACTGGGCCGTTTGCTCCACGGCCACC
TCACACCGTAGCCTCTCACCCCAGCTGCTCCAGCAGATGCCCAGCAAGCCCGAAGGGGCC
GCGAAGACAATTGGGAGTTACGTGCCCGGGCCCCGTAGCCGATCCCCATCGCTAAATAGG
CTGGGCGGTGCCGGCGAGGATGGAAAGAGGCCGCAGCCTCTCTGGCATGTCGGGTCGCCT
TTTGCTCTTGGTGCCAACAAGGACTCACTCTCGGCTTTTGAGTACCCGGGGCCCAAGCGG
AAGCTCTACTCTGCTGTGCCCGGCAGGCTCTTCGTCGCTGTCAAGCCATACCAACCTCAA
GTGGACGGTGAGATCCCCCTTCATCGCGGTGACAGGGTCAAAGTTCTGAGCATTGGTGAA
GGCGGCTTCTGGGAAGGCAGCGCCCGCGGCCATATCGGATGGTTTCCGGCGGAGTGCGTT
GAGGAGGTCCAGTGTAAGCCCAGAGACAGCCAAGCAGAAACCCGCGCTGACCGCAGTAAG
AAGCTTTTCAGGCACTACACAGTGGGCTCCTATGACAGCTTCGACACTTCAAGTGACTGC
ATTATTGAGGAGAAGACCGTGGTCCTGCAGAAAAAAGACAATGAAGGCTTTGGATTCGTG
CTTCGAGGGGCCAAAGCTGACACACCCATTGAAGAATTCACACCAACACCGGCTTTCCCA
GCCCTACAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCGTGGCAAGCCGGACTGCGG
ACCGGGGACTTCTTGATTGAAGTTAATAATGAGAATGTCGTCAAAGTCGGACACAGACAG
GTGGTGAACATGATCCGGCAGGGAGGGAATCACCTGGTCCTTAAGGTGGTCACGGTGACC
AGAAATCTGGACCCCGACGACACAGCCAGGAAGAAGGCTCCCCCACCTCCAAAGCGCGCA
CCGACCACAGCCCTCACACTCCGCTCTAAGTCCATGACCTCGGAGCTGGAGGAGCTCGTC
GATAAAGCCTCAGTCCGAAAGAAGAAGGATAAACCTGAGGAGATAGTCCCGGCCTCCAAG
CCCTCTCGCGCTGCTGAGAACATGGCTGTGGAACCGAGGGTTGCGACGATCAAGCAGCGA
CCCAGCAGCCGCTGTTTCCCGGCGGGCTCCGACATGAACTCTGTGTACGAACGCCAAGGA
ATCGCCGTGATGACTCCCACTGTTCCCGGGAGCCCAAAAGCCCCGTTCCTGGGCATACCT
CGAGGCACGATGCGACGGCAGAAATCAATAGACAGCAGGATCTTTCTATCAGGAATAACA
GAGGAAGAGAGGCAGTTTCTGGCCCCCCCAATGCTGAAGTTTACACGAAGCCTCTCCATG
CCGGACACCTCCGAGGACATCCCCCCTCCACCGCAGTCTGTGCCCCCGTCCCCCCCGCCA
CCTTCCCCAACCACTTACAACTGCCCCAAGTCCCCAACTCCTAGAGTCTACGGGACGATT
AAGCCTGCGTTCAATCAGAATTCCGCCGCCAAGGTGTCCCCCGCTACCAGGTCCGATACC
GTGGCCACCATGATGAGGGAGAAAGGGATGTACTTCAGAAGAGAGCTCGACCGCTACTCC
CTGGACTCTGAAGACCTCTACAGTCGGAATGCTGGCCCGCAAGCTAACTTCCGCAACAAG
CGCGGCCAGATGCCAGAAAACCCATACTCAGAGGTGGGCAAGATCGCCAGCAAAGCGGTC
TACGTCCCCGCCAAGCCCGCCAGGAGGAAGGGCATGTTGGTCAAGCAGTCTAACGTGGAG
GACAGCCCCGAGAAGACGTGCTCCATCCCTATCCCGACCATCATCGTGAAGGAGCCGAGT
ACCAGTAGTAGCGGCAAGAGTAGCCAGGGCAGCAGCATGGAAATCGACCCCCAAGCTCCC
GAGCCACCGAGCCAGCTGAGGCCTGACGAAAGCCTGACCGTCAGCAGCCCTTTTGCAGCC
GCCATCGCCGGAGCCGTCCGCGACCGTGAGAAGCGCTTGGAAGCCCGGAGGAACTCACCG
GCCTTCCTCTCCACTGACCTGGGGGATGAAGATGTGGGCCTGGGGCCTCCCGCACCCAGG
ACGCGCCCCTCCATGTTCCCCGAGGAGGGGGACTTTGCTGACGAGGACAGTGCTGAGCAG
CTCTCATCGCCCATGCCGAGTGCTACCCCCAGGGAGCCCGAAAACCATTTTGTGGGTGGC
GCCGAGGCTAGTGCTCCGGGTGAGGCTGGGAGGCCGCTCAATTCCACCAGTAAAGCTCAG
GGGCCCGAGAGCAGCCCAGCCGTGCCCTCAGCGAGCAGCGGTACAGCCGGCCCTGGCAAT
TATGTCCACCCACTCACAGGGCGGCTGTTGGATCCCAGTTCCCCTCTGGCTCTCGCACTC
TCCGCCAGGGACCGCGCCATGAAGGAGTCTCAACAGGGACCCAAAGGTGAGGCCCCCAAG
GCCGACCTCAACAAACCTCTCTACATTGATACCAAAATGCGGCCCTCCCTTGATGCCGGC
TTCCCTACGGTCACCAGGCAGAACACCCGGGGACCCCTGAGGCGACAGGAGACGGAGAAC
AAGTACGAGACAGACCTGGGCCGAGACAGGAAAGGCGATGACAAGAAGAACATGCTGATC
GATATCATGGACACGTCCCAACAGAAGTCCGCAGGCCTACTGATGGTGCACACCGTGGAC
GCCACTAAGCTGGACAACGCCCTGCAGGAAGAGGACGAGAAGGCCGAGGTGGAAATGAAG
CCAGACAGCTCGCCGTCCGAGGTGCCAGAAGGTGTTTCCGAAACCGAAGGTGCTTTACAG
ATCTCCGCTGCCCCCGAGCCTACCACCGTCCCCGGCAGAACCATCGTCGCGGTGGGGTCT
ATGGAAGAGGCTGTGATTTTGCCTTTCCGCATCCCTCCCCCCCCACTGGCATCCGTGGAC
TTGGATGAGGATTTTATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCTAATAGT
TTTGATATCCCCGATGACCGGGCAGCTTCTGTCCCTGCTCTGTCAGACCTAGTGAAGCAG
AAGAAAAGCGACACACCTCAGTCTCCTTCGTTGAACTCTAGCCAGCCAACCAACTCTGCA
GACAGCAAGAAGCCAGCTAGTCTTTCAAACTGTTTGCCTGCCTCATTCCTGCCACCCCCT
GAAAGCTTTGACGCCGTGGCCGACTCTGGGATCGAGGAGGTGGATAGCCGGAGTAGCTCT
GACCACCACCTTGAGACGACCAGCACAATCTCCACCGTGTCTAGCATCTCTACCTTGTCT
TCCGAGGGTGGAGAGAATGTGGATACCTGCACCGTCTATGCAGATGGGCAAGCATTTATG
GTGGACAAACCCCCAGTACCTCCTAAGCCAAAGATGAAGCCCATCATTCACAAAAGTAAT
GCACTTTATCAAGACGCGCTCGTGGAAGAAGATGTAGATAGCTTTGTTATCCCTCCGCCC
GCTCCCCCGCCGCCCCCTGGCAGTGCCCAACCGGGAATGGCCAAGGTTCTCCAGCCTAGG
ACCTCCAAGTTGTGGGGCGATGTCACAGAGATTAAAAGCCCGATTCTCTCAGGCCCAAAG
GCCAACGTTATTAGTGAATTGAACTCCATCCTACAGCAAATGAACCGAGAGAAATTGGCA
AAGCCGGGGGAAGGACTGGATTCCCCAATGGGAGCCAAATCCGCCAGCCTCGCTCCAAGA
TCCCCGGAGATCATGAGCACCATCTCAGGTACAAGGAGTACGACCGTCACCTTCACTGTT
CGCCCCGGCACCTCCCAACCCATCACCCTGCAGAGCCGGCCACCCGACTATGAAAGCAGG
ACCTCAGGAACCAGACGTGCACCAAGCCCAGTGGTGTCGCCAACAGAGATGAACAAAGAA
ACCCTCCCCGCCCCCCTGTCGGCTGCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTC
TTTAGCCTGCCAAGCCAGCCCCCTTCTGGGGATCTATTTGGCCTGAACCCAGCGGGACGC
AGTAGGTCGCCATCCCCCTCGATACTGCAACAACCAATCTCAAATAAGCCTTTTACAACT
AAACCTGTCCACCTGTGGACTAAACCAGATGTGGCCGATTGGCTGGAAAGTCTAAACTTG
GGAGAACATAAAGAGGCTTTTATGGACAATGAGATCGATGGAAGTCACTTACCAAATCTG
CAGAAGGAGGACCTGATTGATCTTGGGGTAACTAGAGTGGGCCACAGAATGAACATAGAA
AGGGCTTTGAAACAGCTGCTGGACAGA
Protein sequence predicted based on nucleotide sequence
MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATAEKPGGARTEESQGNTLVIR VVIHDLQQTKCIRFNPDATVWVAKQRILCTLTQSLKDVLNYGLFQPASNGRDGKFLDEER LLREYPQPVGEGVPSLEFRYKKRVYKQASLDEKQLAKLHTKTNLKKCMDHIQHRLVEKIT KMLDRGLDPNFHDPETGETPLTLAAQLDDSVEVIKALKNGGAHLDFRAKDGMTALHKAAR ARNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGGDPYCCELLLHEHATVCCKDENGWH EIHQACRYGHVQHLEHLLFYGADMSAQNASGNTALHICALYNQDSCARVLLFRGGNKELK NYNSQTPFQVAIIAGNFELAEYIKNHKETDIVPFREAPAYSNRRRRPPNTLAAPRVLLRS NSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPEGAAKTIGSYVPGPRSRSPSLNR LGGAGEDGKRPQPLWHVGSPFALGANKDSLSAFEYPGPKRKLYSAVPGRLFVAVKPYQPQ VDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSK KLFRHYTVGSYDSFDTSSDCIIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFP ALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVT RNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKASVRKKKDKPEEIVPASK PSRAAENMAVEPRVATIKQRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIP RGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPP PSPTTYNCPKSPTPRVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRELDRYS LDSEDLYSRNAGPQANFRNKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVE DSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQAPEPPSQLRPDESLTVSSPFAA AIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQ LSSPMPSATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGN YVHPLTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAG FPTVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVD ATKLDNALQEEDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGS MEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQ KKSDTPQSPSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVDSRSSS DHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPIIHKSN ALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPILSGPK ANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTV RPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDV FSLPSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNL GEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
