Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746319
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3900nts
Open reading frame: 1 to 3900
ATGCCCAAGATAGTCCTGAATGGTGTCACAGTAGACTTCCCTTTCCAGCCCTACAAATGC
CAACAGGAGTACATGACCAAAGTCCTGGAATGTCTGCAGCAGAAGGTGAATGGCATCCTG
GAGAGCCCTACGGGTACAGGCAAGACGCTGTGCCTGCTCTGCACCACGCTGGCCTGGCGA
GAACACCTCCGAGACGGCATCTCTGCCCGAAAGATTGCCGAGAGGGCGCAAGGAGAGCTT
TTCCCGGATAGGGCCTTGTCATCCTGGGGCAACGCTGCTGCTGCTGCTGGAGATCCCATA
GCTTGCTACACGGACATTCCAAAGATCATTTACGCCTCCAGGACCCACTCGCAACTGACC
CAGGTCATCAACGAGCTTCGGAACACCTCTTACCGGCCTAAGGTGTGTGTGCTCGGCTCC
CGCGAGCAGCTGTGCATCCATCCTGAGGTCAAGAAACAAGAGAGTAACCATCTACAGATC
CACTTGTGCCGTAAGAAGGTCGCAAGTCGCTCCTGTCATTTCTACAACAACGTAGAAGAA
AAAAGCCTGGAGCAGGAGCTTGCTAGCCCCATCCTGGACATTGAGGACTTGGTCAAGAGC
GGAAGTAAGCACAGGGTGTGCCCTTACTACCTGTCCCGGAACCTGAAGCAGCAAGCCGAC
ATCATATTCATGCCGTACAACTACTTGTTGGATGCCAAGAGCCGCCGAGCCCACAACATT
GACCTGAAGGGGACAGTCGTGATCTTTGACGAAGCTCACAACGTGGAGAAGATGTGTGAA
GAATCGGCATCCTTTGACCTGACTCCCCATGACCTGGCTTCAGGACTCGACGTCATAGAC
CAGGTGCTCGAGGAGCAGACCAAGGCAGCGCAGCAGGGTGAGCCCCACCCGGAGTTCAGC
GCGGACTCCCCCAGCCCAGGGCTGAACATGGAGCTGGAAGACATTGCAAAGCTGAAGATG
ATCCTGCTCCGCCTGGAGGGGGCCATCGATGCTGTTGAGCTGCCTGGAGACGACAGCGGT
GTCACCAAACCAGGGAGCTACATCTTTGAGCTGTTTGCCGAAGCCCAGATCACGTTTCAG
ACCAAGGGCTGCATCCTGGACTCGCTGGACCAGATCATCCAGCACCTCGCAGGCCGTGCT
GGAGTGTTCACCAACACGGCCGGACTGCAGAAGCTGGCGGACATAATCCAGATTGTGTTC
AGTGTGGACCCCTCCGAGGGCAGTCCTGGTTCCCCAGCAGGGCTGGGGGCCTTGCAGTCC
TATAAGGTGCACATCCATCCTGATGCTGGTCACCGGAGGACGGCTCAGCGGTCTGATGCC
TGGAGTACCACCGCAGCCCGGAAGCGAGGGAAGGTGCTCAGCTACTGGTGCTTTAGTCCC
GGCCACAGCATGCACGAGCTGGTCCGCCAGGGCGTCCGCTCCCTCATCCTTACCAGCGGA
ACGCTGGCCCCCGTGTCTTCCTTTGCTCTGGAGATGCAGATCCCTTTCCCAGTGTGCCTG
GAGAATCCACACATCATCGACAAGCACCAGATCTGGGTGGGGGTCGTCCCCAGAGGCCCC
GATGGAGCCCAGTTGAGCTCCGCGTTTGACAGACGGTTTTCCGAGGAGTGCTTATCCTCC
CTGGGGAAGGCCCTTGGCAACATCGCACGCGTGGTGCCCTATGGGCTCCTGATCTTCTTC
CCTTCCTATCCCGTCATGGAGAAGAGCCTGGAGTTCTGGCGGGCCCGCGATTTAGCCAGG
AAGATGGAGGCGCTGAAGCCGCTGTTTGTGGAGCCCAGGAGCAAAGGCAGCTTCTCCGAG
ACTATCAGTGCTTACTATGCAAGGGTTGCCGCCCCTGGGTCTACCGGCGCCACCTTCCTG
GCGGTCTGCCGGGGCAAGGCCAGCGAGGGACTTGACTTCTCAGACACGAATGGACGTGGT
GTGATTGTCACGGGCCTGCCGTACCCCCCACGCATGGACCCCCGGGTTGTCCTCAAGATG
CAGTTCCTGGATGAGATGAAGGGCCAGGGTGGGGCTGGGGGCCAGTTCCTTTCTGGGCAG
GAGTGGTACCGGCAGCAGGCGTCCAGGGCTGTGAACCAGGCCATTGGGCGAGTGATCCGG
CACCGCCAGGACTACGGAGCTGTCTTCCTCTGTGACCACAGGTTCGCCTTTGCTGACGCA
AGAGCCCAACTGCCCTCTTGGGTCCGTCCCCACGTGAGGGTGTATGACAACTTTGGCCAT
GTGATCCGAGACGTGGCCCAGTTCTTCCGTGTTGCCGAGCGAACTATGCCAGCGCCGGCC
CCCCGGGCTACAGCACCCAGTGTCCGTGGTGAAGATGCCGTCAGCGAGGCCAAGTCGCCT
GGACCCTTCTTCTCCACCAGGAAAGCTAAGAGCCTCGACCTGCATGTCCCCAGCCTGAAG
CAGAGGTCTTCAGGGTCACCAGCTGCCGGGGACCCCGAGAGTAGCCTGTGTGTGGAGTAT
GAGCAGGAGCCAGTTCCTGCTCGGCAGAGGCCTAGGGGGCTGCTGGCTGCCCTTGAGCAC
AGTGAACAGCGGGCGGGGAGTCCTGGCGAGGAGCAGGCTCACAGCTGTTCCACCCTGTCC
CTCCTGTCTGAGAAGAGGCCGGCAGAAGAACCGCGAGGAGGGAGGAAGAAAATCCGGCTG
GTCAGCCACCCGGAGGAGCCCGTGGCTGGTGCCCAGACGGACCGGGCCAAGCTCTTCATG
GTGGCCGTCAAGCAGGAGTTGAGCCAAGCCAACTTTGCCACCTTCACCCAGGCCCTGCAG
GACTACAAGGGTTCCGATGACTTCGCCGCCCTGGCCGCCTGTCTCGGCCCCCTCTTTGCT
GAGGACCCCAAGAAGCACAACCTGCTCCAAGGCTTCTACCAGTTTGTGCGGCCCCACCAT
AAGCAGCAGTTTGAGGAGGTCTGTATCCAGCTGACAGGACGAGGCTGTGGCTATCGGCCT
GAGCACAGCATTCCCCGAAGGCAAAGGGCACAGCCAGTCCTGGACCCCACTGGAAGAACG
GCGCCGGATCCCAAGCTGACCGTGTCCACGGCTGCAGCTCAGCAGTTGGACCCCCAAGAG
CACCTGAACCAGGGCAGGCCCCACCTGTCACCCAGGCCACCTCCAACAGGAGATCCTGGC
AGCCAACCCCAGTGGGGGTCTGGAGTTCCTAGAGCAGGCAAGCAAGGCCAGCACGCCGTG
AGCGCTTACCTGGCTGATGCCCGAAGGGCCCTGGGGTCCGCGGGCTGTAGCCAACTCTTG
GCAGCTCTGACAGCCTATAAGCAAGACGACGACCTCGACAAGGTGCTGGCTGTCTTGGCA
GCCCTGACCACTGCAAAGCCAGAGGACTTCCCTCTGCTGCACAGATTCAGCATGTTTGTG
CGTCCACACCACAAGCAGCGCTTCTCACAGACGTGCACAGACCTGACCGGCCGGCCCTAC
CCTGGCATGGAGCCTCCGGGACCCCAGGAGGAGAGGCTTGCAGTGCCTCCTGTGCTTACC
CACAGAGCTCCACAACCAGGCCCCTCACGGTCCGAGAAGACAGGGAAGACCCAGAGCAAG
ATCTCGTCCTTCCTTAGACAGAGGCCAGCAGGGACTGTCGGGGCGGGCGGTGAGGATGCA
GGTCCCAGCCAGTCCTCAGGACCTCCTCACGGGCCTGCAGCATCTGAGTGGGGTGAGCCT
CATGGGAGAGACATCGCTGGCCAGCAGGCCACGGGAGCTCCCGGTGGGCCTCTTTCAGCA
GGCTGTGTGTGCCAGGGCTGTGGGGCAGAGGACGTGGTTCCCTTCCAGTGCCCTGCCTGT
GACTTCCAGCGCTGCCAAGCCTGCTGGCAACGGCACCTTCAGGCCTCTAGGATGTGCCCA
GCCTGCCACACCGCCTCCAGGAAGCAGAGCGTCATGCAGGTCTTCTGGCCAGAGCCCCAG
Protein sequence predicted based on nucleotide sequence
MPKIVLNGVTVDFPFQPYKCQQEYMTKVLECLQQKVNGILESPTGTGKTLCLLCTTLAWR EHLRDGISARKIAERAQGELFPDRALSSWGNAAAAAGDPIACYTDIPKIIYASRTHSQLT QVINELRNTSYRPKVCVLGSREQLCIHPEVKKQESNHLQIHLCRKKVASRSCHFYNNVEE KSLEQELASPILDIEDLVKSGSKHRVCPYYLSRNLKQQADIIFMPYNYLLDAKSRRAHNI DLKGTVVIFDEAHNVEKMCEESASFDLTPHDLASGLDVIDQVLEEQTKAAQQGEPHPEFS ADSPSPGLNMELEDIAKLKMILLRLEGAIDAVELPGDDSGVTKPGSYIFELFAEAQITFQ TKGCILDSLDQIIQHLAGRAGVFTNTAGLQKLADIIQIVFSVDPSEGSPGSPAGLGALQS YKVHIHPDAGHRRTAQRSDAWSTTAARKRGKVLSYWCFSPGHSMHELVRQGVRSLILTSG TLAPVSSFALEMQIPFPVCLENPHIIDKHQIWVGVVPRGPDGAQLSSAFDRRFSEECLSS LGKALGNIARVVPYGLLIFFPSYPVMEKSLEFWRARDLARKMEALKPLFVEPRSKGSFSE TISAYYARVAAPGSTGATFLAVCRGKASEGLDFSDTNGRGVIVTGLPYPPRMDPRVVLKM QFLDEMKGQGGAGGQFLSGQEWYRQQASRAVNQAIGRVIRHRQDYGAVFLCDHRFAFADA RAQLPSWVRPHVRVYDNFGHVIRDVAQFFRVAERTMPAPAPRATAPSVRGEDAVSEAKSP GPFFSTRKAKSLDLHVPSLKQRSSGSPAAGDPESSLCVEYEQEPVPARQRPRGLLAALEH SEQRAGSPGEEQAHSCSTLSLLSEKRPAEEPRGGRKKIRLVSHPEEPVAGAQTDRAKLFM VAVKQELSQANFATFTQALQDYKGSDDFAALAACLGPLFAEDPKKHNLLQGFYQFVRPHH KQQFEEVCIQLTGRGCGYRPEHSIPRRQRAQPVLDPTGRTAPDPKLTVSTAAAQQLDPQE HLNQGRPHLSPRPPPTGDPGSQPQWGSGVPRAGKQGQHAVSAYLADARRALGSAGCSQLL AALTAYKQDDDLDKVLAVLAALTTAKPEDFPLLHRFSMFVRPHHKQRFSQTCTDLTGRPY PGMEPPGPQEERLAVPPVLTHRAPQPGPSRSEKTGKTQSKISSFLRQRPAGTVGAGGEDA GPSQSSGPPHGPAASEWGEPHGRDIAGQQATGAPGGPLSAGCVCQGCGAEDVVPFQCPAC DFQRCQACWQRHLQASRMCPACHTASRKQSVMQVFWPEPQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
