Gene:
Gene Symbol:
ZFYVE9
Gene Name:
zinc finger, FYVE domain containing 9
Original Clone ID:
FLH170642.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00075991
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAATTACTTCCAAGCAGAAGCTTACAACCTGGACAAGGTGTTAGATGAATTTGAA
CAAAACGAAGATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTA
GATCCCCCTTCTCACCGGCTGTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCA
GTTTCTAATGAGTCACAACCACAACTGAAAGTCTTCTCCCTGGCTCATTCAGCTCCCCTG
ACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACTGTAATCTAAATCCAGAGATT
GCCACAATGTGGATTGATGAAAATGCTGTTGCAGAAGACCAGTTAATTAAGAGAAACTAT
AGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCT
TGTCTGCCAGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGG
ACATTACAAAACGATTTACAGGATTGTAATAATTATAATAGTCAATCCCTTATGGATGCT
TTTAGCTGTTCACTGGATAATGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAAT
GAGTCCACTGAAAAAGATATGAATTCAGAGAAACAAATGGATCCATTGAATAGACCGAAA
ACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCATCTGATAGTCTAGCCAGTGTC
TGTTCCCCTTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTCCATGTCTGCG
ATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGATGGATGTCCTGCT
GTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCT
AGGACAGATCTAGGGAGTCCAAATTCCTTTTCCCACATGAGTGAGGGGATTTTGATGAAA
AAAGAGCCAGCAGAGGAGAGCACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTT
CTCAAACCAGACATGCCTAATGGGTCTGGAAGGAATAATGACTGTGAACGGTGTTCAGAT
TGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAGGTTATGAACATGAAGAAACT
CTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCAGGACATGACT
AATTGGAAGTTGACTAAACTAAATGAGATGAATGATAGCCAAGTAAACGAAGAAAAGGAA
AAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTT
GGATTGGCAGATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGT
GATTTCTCCACTGTTATAGACACACCAGCAGCAAATTATCTATCTAATGGTTGTGATTCC
TATGGAATGCAAGACCCAGGTGTTTCTTTTGTTCCAAAGACTTTACCCTCCAAAGAAGAT
TCAGTAACAGAAGAAAAAGAAATAGAGGAAAGCAAGTCAGAATGCTACTCAAATATTTAT
GAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAGCACTGGTGAC
CTAATGAAGAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAA
TCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTGCAAGA
CCCAAGCAACCTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAA
AATGACTTTCCTGCAAACAGTGGAAATAATACTAAAAATAAAAATGATATTCTTGGGAAA
GCAAAATTAGGGGAAAACTCAGCAACCAATGTATGCAGTCCATCTTTGGGAAACATCTCT
AATGTCGATACAAATGGGGAACATTTAGAAAGTTATGAGGCTGAGATCTCCACTAGACCA
TGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGTCAGTTTGGAATT
TCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCT
CAGGCTCCAAATTGCATGAAATGTGAAGCCAGGTTTACATTCACCAAAAGGAGGCATCAC
TGCAGAGCATGTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTA
TACATGGACAGAAAGGAAGCTAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGCT
CAAGCCTGGGAGAACATGATGAGTGCCTCAAGCCAGAGCCCTAACCCTAACAATCCTGCT
GAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGGCCTCAGGAGCTCTGAGCTCT
CCACCTCCCACTGTGATGGTACCTGTGGGAGTTTTAAAGCACCCTGGAGCAGAAGTGGCT
CAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTGCCCAATGGAGAAGTT
GCTGATGCAGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCA
CACGACCCAGTCAAGCCAGTAACTACCAGTCCTCTACCAGCAGAGACGGATATTTGTCTA
TTCTCTGGGAGTATAACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCT
GAAGATGGCCTTCCTCCCATTCTCATCTCCACTGGTGTAAAAGGAGACTATGCTGTGGAA
GAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGGATGGTGGCCCTGACCCACTT
GTATTTGTTTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTATGTGAACAGG
AAGTGCTGGTGTTTCACAACCAAGGGAATGCATGCAGTGGGTCAGTCTGAGATAGTCATT
CTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTG
CAGCTTTATCGGGATGCTCTGGCAGGGAATGTGGTGAGCAACTTGGGACATTCCTTCTTC
AGTCAAAGTTTCCTTGGCAGTAAAGAACATGGTGGATTCTTATATGTGACATCTACCTAC
CAGTCACTGCAAGACCTAGTACTCCCAACCCCACCTTACTTGTTTGGGATTCTTATCCAG
AAATGGGAAACTCCTTGGGCTAAAGTATTTCCTATCCGTCTGATGTTGAGACTTGGAGCT
GAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCCATTGTTTGGA
GAGACGGGGCATACCATCATGAATCTTCTTGCAGACTTCAGAAATTACCAGTATACCTTG
CCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACTAGCATCAAAATTCCC
AGCAACAGATACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCA
GGAGGTGCCTGCTTCAATGAAAAGGCAGACTCTCATCTTGTGTGTGTACAGAATGATGAT
GGAAACTATCAGACCCAGGCTATCAGTATTCACAATCAGCCCAGAAAAGTGACTGGTGCC
AGTTTCTTTGTGTTCAGTGGCGCTCTGAAATCCTCTTCTGGATACCTTGCCAAGTCCAGT
ATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATGGATTCCTTGAGGCAG
GCACTGCGAGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCC
CAGGAGCACATCCACATCCAGTGGGTGGATGATGACAAGAACGTTAGCAAGGGTGTCGTA
AGTCCTATAGATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCA
GAATATAAAGCAAATGGAAAAGTAATCAGATGGACAGAGGTGTTTTTCCTAGAAAACGAT
GACCAGCACAATTGCCTCAGTGATCCTGCAGATCACAGTAGATTGACTGAGCATGTTGCC
AAAGCTTTTTGCCTTGCTCTCTGTCCTCACCTGAAACTTCTGAAGGAAGATGGAATGACC
AAACTGGGACTACGTGTGACACTTGACTCAGATCAGGTTGGCTATCAAGCAGGGAGCAAT
GGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATC
CATGGAGGGGCCTGCCAGCTTAGTGAGGGCCCCGTTGTCATGGAACTCATCTTTTATATT
CTGGAAAACATCGTA
Protein sequence predicted based on nucleotide sequence
MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESA VSNESQPQLKVFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNY SWDDQCSAVEVGEKKCGNLACLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDA FSCSLDNENRQTDQFSFSINESTEKDMNSEKQMDPLNRPKTEGRSVNHLCPTSSDSLASV CSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPAVKKQENYIPDEDLTGKISSP RTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSGRNNDCERCSD CLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKE KFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDS YGMQDPGVSFVPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGD LMKKNYLHNFCSQVPSVLGQSSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQ NDFPANSGNNTKNKNDILGKAKLGENSATNVCSPSLGNISNVDTNGEHLESYEAEISTRP CLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHH CRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPA EYCSTIPPLQQAQASGALSSPPPTVMVPVGVLKHPGAEVAQPREQRRVWFADGILPNGEV ADAAKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAETDICLFSGSITQVGSPVGSAMNLIP EDGLPPILISTGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFVLNANLLSMVKIVNYVNR KCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQLYRDALAGNVVSNLGHSFF SQSFLGSKEHGGFLYVTSTYQSLQDLVLPTPPYLFGILIQKWETPWAKVFPIRLMLRLGA EYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKTSIKIP SNRYNEMMKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGA SFFVFSGALKSSSGYLAKSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEEP QEHIHIQWVDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEYKANGKVIRWTEVFFLEND DQHNCLSDPADHSRLTEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQAGSN GQPLPSQYMNDLDSALVPVIHGGACQLSEGPVVMELIFYILENIV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
12
End on reference sequence
4289
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
