SKD3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  CLPB
Gene Name:  ClpB caseinolytic peptidase B homolog (E. coli)
Original Clone ID:
FLH194217.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00076279
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2121nts
Open reading frame: 1 to 2121

ATGCTGGGGTCCCTGGTGTTGAGGAGAAAAGCACTGGCGCCACGGCTACTCCTCCGGCTG
CTCAGGTCCCCAACGCTCCGGGGCCATGGAGGTGCTTCCGGCCGGAATGTGACTACTGGG
AGTCTCGGGGAGCCGCAGTGGCTGAGGGTAGCCACCGGGGGGCGCCCTGGAACATCGCCG
GCCTTGTTCTCCGGACGTGGGGCAGCCACCGGGGGGCGCCAGGGAGGACGCTTCGATACC
AAATGCCTCGCGGCTGCCACTTGGGGACGCCTTCCTGGTCCCGAAGAAACACTCCCAGGA
CAGGACAGCTGGAACGGGGTCCCCAGCAGGGCCGGACTGGGCATGTGCGCCCTGGCCGCA
GCGCTGGTGGTTCATTGCTACAGCAAGAGTCCGTCCAACAAGGATGCAGCCCTGTTGGAA
GCTGCCCGTGCCAACAATATGCAAGAAGTCAGCAGGCTGTTGTCAGAAGGTGCAGATGTC
AATGCAAAGCACAGACTTGGCTGGACAGCACTCATGGTGGCAGCCATCAACCGAAACAAC
AGTGTGGTACAGGTCCTGCTTGCTGCTGGGGCTGATCCAAACCTTGGAGATGATTTCAGC
AGTGTTTACAAGACTGCCAAGGAACAGGGAATCCATTCTTTGGAAGATGGGGGACAGGAC
GGTGCAAGCCGGCACATCACAAACCAGTGGACAAGTGCCCTGGAGTTCAGGAGATGGCTA
GGACTCCCCGCTGGCGTCCTGATCACCCGAGAGGATGACTTCAACAACAGGCTGAACAAC
CGCGCCAGTTTCAAGGGCTGCACGGCCTTGCACTATGCTGTTCTTGCTGATGACTACCGC
ACTGTCAAGGAGCTGCTTGATGGAGGAGCCAACCCCCTGCAGAGGAATGAAATGGGACAC
ACACCCTTGGATTATGCCCGAGAAGGGGAAGTGATGAAGCTTCTGAGGACTTCTGAAGCC
AAGTACCAAGAGAAGCAGCGGAAGCGTGAGGCTGAGGAGCGGCGCCGCTTCCCCCTGGAG
CAGCGACTAAAGGAGCACATCATTGGCCAGGAGAGCGCCATCGCCACAGTGGGTGCTGCG
ATCCGGAGGAAGGAGAATGGCTGGTACGATGAAGAACACCCTCTGGTCTTCCTCTTCTTG
GGATCATCTGGAATAGGAAAAACAGAGCTGGCCAAGCAGACAGCCAAATATATGCACAAA
GATGCTAAAAAGGGCTTCATCAGGCTGGACATGTCCGAGTTCCAGGAGCGACACGAGGTG
GCCAAGTTTATTGGGTCTCCACCAGGCTACGTTGGCCATGAGGAGGGTGGCCAGCTGACC
AAGAAGTTGAAGCAGTGCCCCAATGCTGTGGTGCTCTTTGATGAAGTAGACAAGGCCCAT
CCAGATGTGCTCACCATCATGCTGCAGCTGTTTGATGAGGGCCGGCTGACAGATGGAAAA
GGGAAGACCATTGATTGCAAGGACGCCATCTTCATCATGACCTCCAATGTGGCCAGCGAC
GAGATCGCACAGCACGCGCTGCAGCTGAGGCAGGAAGCTTTGGAGATGAGCCGTAACCGT
ATTGCCGAAAACCTGGGGGATGTCCAGATAAGTGACAAGATCACCATCTCAAAGAACTTC
AAGGAGAATGTGATTCGCCCTATCCTGAAAGCTCACTTCCGGAGGGATGAGTTTCTGGGA
CGGATCAATGAGATCGTCTACTTCCTCCCCTTCTGCCACTCGGAGCTCATCCAACTCGTC
AACAAGGAACTAAACTTCTGGGCCAAGAGAGCCAAGCAAAGGCACAACATCACGCTGCTC
TGGGACCGCGAGGTGGCAGATGTGCTGGTCGACGGCTACAATGTGCACTATGGCGCCCGC
TCCATCAAACATGAGGTAGAACGCCGTGTGGTGAACCAGCTGGCAGCAGCCTATGAGCAG
GACCTGCTGCCAGGGGGCTGTACTTTGCGCATCACGGTGGAGGACTCAGACAAGCAGCTA
CTCAAAAGCCCAGAACTGCCCTCACCCCAGGCTGAGAAGCGCCTCCCCAAGCTGCGTCTG
GAGATCATCGACAAGGACAGCAAGACTCGCAGACTGGACATCCGGGCACCACTGCACCCT
GAGAAGGTGTGCAACACCATC

Protein sequence predicted based on nucleotide sequence

MLGSLVLRRKALAPRLLLRLLRSPTLRGHGGASGRNVTTGSLGEPQWLRVATGGRPGTSP
ALFSGRGAATGGRQGGRFDTKCLAAATWGRLPGPEETLPGQDSWNGVPSRAGLGMCALAA
ALVVHCYSKSPSNKDAALLEAARANNMQEVSRLLSEGADVNAKHRLGWTALMVAAINRNN
SVVQVLLAAGADPNLGDDFSSVYKTAKEQGIHSLEDGGQDGASRHITNQWTSALEFRRWL
GLPAGVLITREDDFNNRLNNRASFKGCTALHYAVLADDYRTVKELLDGGANPLQRNEMGH
TPLDYAREGEVMKLLRTSEAKYQEKQRKREAEERRRFPLEQRLKEHIIGQESAIATVGAA
IRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEV
AKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGK
GKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKITISKNF
KENVIRPILKAHFRRDEFLGRINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLL
WDREVADVLVDGYNVHYGARSIKHEVERRVVNQLAAAYEQDLLPGGCTLRITVEDSDKQL
LKSPELPSPQAEKRLPKLRLEIIDKDSKTRRLDIRAPLHPEKVCNTI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2183
Start on reference sequence 60
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable