UBA2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  UBA2
Gene Name:  ubiquitin-like modifier activating enzyme 2
Original Clone ID:
FLH197169.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 25072996
Title: Analysis of Changes in SUMO-2/3 Modification during Breast Cancer Progression and Metastasis.
Authors:
HIP
HsCD00076329
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1920nts
Open reading frame: 1 to 1920

ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGGGGGCCGGGTG
CTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCAAGAATCTCGTGCTCACCGGT
TTCTCCCACATCGACCTGATTGATCTGGATACTATTGATGTAAGCAACCTCAACAGACAG
TTTTTGTTTCAAAAGAAACATGTTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTA
CTGCAGTTTTACCCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGAC
TATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGATAACAGAGCT
GCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATGTTCCTCTTATTGAAAGTGGA
ACAGCTGGGTATCTTGGACAAGTAACTACTATCAAAAAGGGTGTGACCGAGTGTTATGAG
TGTCATCCTAAGCCGACCCAGAGAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCA
GAACCTATACATTGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAA
GATGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTGGGAACCAACG
GAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTGACATTAAACGTATTTCTACT
AAGGAATGGGCTAAATCAACTGGATATGATCCAGTTAAACTTTTTACCAAGCTTTTTAAA
GATGACATCAGGTATCTGTTGACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTT
CCGTTGGACTGGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAG
AATGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAGCTATGCACGT
CTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAGCAGAAAAGGGGGATGGAGCT
GAGCTCATATGGGATAAGGATGACCCATCTGCAATGGATTTTGTCACCTCTGCTGCAAAC
CTCAGGATGCATATTTTCAGTATGAATATGAAGAGTAGATTTGATATCAAATCAATGGCA
GGGAACATTATTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTG
GAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTTTTTGAATAAA
CAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCACTGGATCCTCCCAACCCCAAT
TGTTATGTATGTGCCAGCAAGCCAGAGGTGACTGTGCGGCTGAATGTCCATAAAGTGACT
GTTCTCACCTTACAAGACAAGATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTC
CAAATTGAAGATGGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCT
AATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCTTCAAGCAGAT
GACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTCATAGTGAAGACCTAGGAAAG
GACGTTGAATTTGAAGTTGTTGGTGATGCCCCGGAAAAAGTGGGGCCCAAACAAGCTGAA
GATGCTGCCAAAAGCATAACCAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACA
GCTCAAGAGCAAGATGACGTTCTCATAGTTGATTCGGATGAAGAAGATTCTTCAAATAAT
GCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAAAGAGAATCTC
AGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTGATGATGTCATAGCATTAGAT

Protein sequence predicted based on nucleotide sequence

MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQ
FLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRA
ARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPS
EPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRIST
KEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQ
NEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAAN
LRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNK
QPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDV
QIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGK
DVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNN
ADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1971
Start on reference sequence 49
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable