Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00780188
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 1908nts
Open reading frame: 644 to 2551
TGAGCCAGCAGTTTGTGCAACCAGAGGAGCGCAGGCAGGGTTCCCTGCTGGGGCCCGGGC
TGCCCAGCCATGCTTTGGGCACTCTGGCCAAGGTGGCTGGCAGACAAGATGCTGCCCCTC
CTGGGGGCAGTGCTGCTTCAGAAGAGAGAGAAGAGGGGCCCTCTGTGGAGGCACTGGCGG
CGGGAAACCTACCCATACTATGACCTCCAGGTGAAGGTGCTGAGGGCCACAAACATCCGG
GGCACAGACCTGCTGTCCAAAGCCGACTGCTATGTGCAACTGTGGCTGCCCACGGCGTCC
CCAAGCCCTGCCCAGACTAGGATAGTGGCCAACTGCAGTGACCCCGAGTGGAATGAGACC
TTCCACTACCAGATCCATGGTGCTGTGAAGAACGTCCTGGAGCTCACCCTCTATGACAAG
GACATCCTGGGCAGCGACCAGCTCTCTCTGCTCCTGTTTGACCTGAGAAGCCTCAAGTGT
GGCCAACCTCACAAACACACCTTCCCACTCAACCACCAGGATTCACAAGAGCTGCAGGTG
GAATTTGTTCTGGAGAAGAGCCAGGTGCCTGCATCTGAAGTCATCACCAACGGGGTTCTG
GTGGCTCACCCCTGTCTGAGAATCCAGGGCACGCTCCGGGGAGATGGGACAGCCCCACGG
GAAGAGTACGCCTCCCACAGAGCCAGGCCTCCCACCCACCTTTACCTTCCACGTGAACCC
AGTGCTGAGCTCCAGGCTACACGTGGAGCTGATGGAGCTGCTGGCAGCTGTGCAGAGTGG
CCCCAGCGCAGAGTTGGAGGCTCAGACCAGCAAGCTGGGCGAGGGGGGCATCCTGCTCTC
CTCTCTGCCCCTAGGCCAGGAGGAACAGTGTTCTGTGGCCCTGGGGGAGGGCCAGGAGGT
GGCTCTGAGCATGAAGGTGGAAATGAGCTCCGGGGACCTAGACCTACGCCTTGGCTTTGA
CCTCTCTGACGGGGAGCAGGAGTTTCTGGACAGGAGGAAGCAGGTCGTGTCCAAGGCCCT
GCAGCAAGTGCTGGGATTGAGTGAGGCTCTGGACAGTGGCCAGGTGCCTGTAGTGGCTGT
GTTGGGTTCCGGGGGTGGAACCCGAGCCATGTCTTCTCTGTACGGCAGCCTGGCAGGGTT
GCAGGAGCTCGGCCTTCTAGACACTGTGACCTACCTGAGTGGGGTCTCTGGGTCTACCTG
GTGCATCTCCACACTCTACAGGGACCCAGCCTGGTCCCAGGTGGCCTTGCAGGGCCCCAT
TGAGCGTGCCCAGGTTCACGTCTGCAGCAGTAAGATGGGAGCTTTGTCCACGGAGCGGCT
ACAGTACTACACTCAGGAACTGGGGGTCCGGGAGCGCAGTGGCCACAGTGTGTCCCTCAT
CGACCTCTGGGGCCTCCTTGTTGAGTATCTCCTGTACCAGGAGGAGAACCCTGCCAAGCT
GTCTGACCAACAGGAGGCGGTCCGCCAGGGTCAGAACCCTTACCCCATTTACACCAGTGT
CAACGTCCGCACCAACTTGAGTGGGGAAGATTTTGCAGAGTGGTGCGAGTTCACGCCCTA
TGAGGTTGGCTTCCCCAAGTACGGGGCTTATGTTCCCACCGAGCTCTTCGGCTCAGAACT
CTTCATGGGACGATTGCTGCAGCTCCAGCCTGAACCCCGGATCTGTTACCTGCAAGGTAT
GTGGGGCAGCGCCTTTGCCACCAGCCTGGATGAGATCTTCCTAAAGACCGCCGGCTCGGG
CCTCAGCTTCCTGGAGTGGTACAGAGGCAGTGTGAATATCACAGACGACTGCCAGAAGCC
TCAGCTGCACAACCCCTCGAGGCTGCGAACGAGGCTCCTCACCCCACAGGGGCCCTTCTC
CCAGGCTGTGCTGGACATATTCACCTCCCGCTTCACTTCCGCCCAGAGCTTTAACTTCAC
CCGGGGTCTCTGCTTGCACAAGGACTATGTGGCTGGCAGGGAGTTCGTGGCCTGGAAAGA
CACACACCCGGACGCCTTCCCCAACCAGCTCACCCCCATGCGGGACTGCCTGTACCTGGT
GGACGGAGGCTTTGCCATCAACTCTCCGTTCCCACTGGCTCTGCTGCCTCAGAGAGCAGT
GGACCTCATTCTGTCCTTTGACTATTCCTTGGAAGCCCCTTTTGAGGTCTTGAAGATGAC
AGAGAAGTACTGCCTGGACCGAGGAATCCCCTTCCCTAGCATCGAGGTGGGCCCTGAGGA
CGTGGAGGAGGCCCGTGAGTGCTATCTGTTTGCCAAGGCTGAGGACCCCCGCTCCCCCAT
TGTGCTGCACTTCCCCCTGGTTAACCGTACCTTCCGCACACACCTGGCCCCAGGTGTGGA
GCGACAAACAGCTGAGGAGAAGGCCTTTGGGGACTTTGTCATCAACAGGCCAGACACCCC
CTATGGCATGATGAACTTCACCTATGAGCCCCAGGACTTTTATCGGCTGGTGGCCCTCAG
TCGATACAACGTCCTGAACAATGTGGAGACCTTGAAGTGCGCCCTCCAGCTGGCTCTGGA
CCGGCACCAGGCTCGGGAGAGGGCAGGGGCCTGACCAAGGCAGGAAGCGGAGGACTGTGA
CAGAGAGGAGACACACTGCTCATGGTCAGGGCTTGTAGAGGGAGGAGCGATGGGGACTCT
GTGCAGGATCTGCTTCCCTTCTCTCCAGGACCTGCCTCGAGGTGCCCCAGGCCCCGGAAA
GCTCTTGCAGAATTGCAGCTTGGACTGGGGCAGGGCTCTCCTTGTGTGTTTTTGGAGAAG
ATGGGCAGTAGATCGCTCCAGGGACTCTTGGGGATGTAGGGCAGAAGAGAACAGCACTCA
TTTCACAGCGGGGTGTGGAGAGAATCAGGTGAGCCACAGAGCCCACCCCAGACACAGAAG
GACCTCAGAGGGCCCAAGTCCTCAGACCCACACAGAACAGGGGCTGAGGGCACTGAGAAG
CCAGCTGTCCTCCTTACACTGAGATGGAAAGCAGAGATGCATCCATCCACACTTCCTGCA
GAGCGGCCCAAGCCCCAACCCCACCTCGAGCTCCTGGATGCACTGCTATCAAGAACAATG
AGGGGCTGAGGGGATGGCCAGCCTATGTTGCTGACTCCATCATCCTAACCCTCCTTCTGC
CTTCTGGTCTCCTCGTGCCTCCTCCCAGATCACCCTTCTCTTCCCAGCGCCCTAAAGCCT
GTGGGGTGATGTCCCATTCTGGCTGCTCCAGGTGGGAGATGTGCGCGTGTCTCCCTGCCA
GTTACCCAGGCTTCACTCTTCGAACCTGGACCACAGTCTCTGGTGATGTGTGTAGTGGCC
ACATCATGCAAATATAGTCTCACCATTCC
Protein sequence predicted based on nucleotide sequence
MGQPHGKSTPPTEPGLPPTFTFHVNPVLSSRLHVELMELLAAVQSGPSAELEAQTSKLGE GGILLSSLPLGQEEQCSVALGEGQEVALSMKVEMSSGDLDLRLGFDLSDGEQEFLDRRKQ VVSKALQQVLGLSEALDSGQVPVVAVLGSGGGTRAMSSLYGSLAGLQELGLLDTVTYLSG VSGSTWCISTLYRDPAWSQVALQGPIERAQVHVCSSKMGALSTERLQYYTQELGVRERSG HSVSLIDLWGLLVEYLLYQEENPAKLSDQQEAVRQGQNPYPIYTSVNVRTNLSGEDFAEW CEFTPYEVGFPKYGAYVPTELFGSELFMGRLLQLQPEPRICYLQGMWGSAFATSLDEIFL KTAGSGLSFLEWYRGSVNITDDCQKPQLHNPSRLRTRLLTPQGPFSQAVLDIFTSRFTSA QSFNFTRGLCLHKDYVAGREFVAWKDTHPDAFPNQLTPMRDCLYLVDGGFAINSPFPLAL LPQRAVDLILSFDYSLEAPFEVLKMTEKYCLDRGIPFPSIEVGPEDVEEARECYLFAKAE DPRSPIVLHFPLVNRTFRTHLAPGVERQTAEEKAFGDFVINRPDTPYGMMNFTYEPQDFY RLVALSRYNVLNNVETLKCALQLALDRHQARERAGA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
