DNASU Plasmid | GTF2A1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GTF2A1

Gene Name: general transcription factor IIA, 1, 19/37kDa

Map: pDONR cofig.pdf

Original Clone ID:

FLH257081.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pDONR221

Format:

FUSION

Source:

HIP

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HIP

HsCD00079572

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1131nts

Open reading frame: 1 to 1131

Reference Sequence Alignment

ATGGCGAACTCGGCAAATACAAACACCGTGCCTAAATTATACAGATCTGTGATTGAAGAT
GTCATTAATGATGTGAGAGACATCTTTCTGGATGATGGAGTGGATGAACAAGTACTGATG
GAACTAAAAACTTTATGGGAAAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCAT
TCAGAAGAGCAGCAGCTTCTACTGCAAGTTCAACAGCAGCATCAACCCCAGCAGCAGCAG
CATCACCACCATCACCATCATCAGCAAGCTCAGCCTCAGCAGACAGTACCTCAGCAAGCG
CAGACCCAGCAGGTTCTTATTCCTGCATCACAGCAAGCCACAGCACCACAAGTTATTGTT
CCAGATTCTAAGTTGATACAGCATATGAATGCATCAAACATGAGTGCTGCTGCTACAGCT
GCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATTAACAAATTCAGGC
CAGCTTCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAGCAG
TCAGTGGTTCTACAACAACAGGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCT
GTTATACAGCAGGTGCTGGCTCCTCTTCCTGGAGGGATTTCACCACAGACAGGTGTCATC
ATCCAGCCTCAGCAAATCTTATTTACAGGAAATAAGACTCAAGTTATACCTACGACAGTG
GCAGCACCTACACCAGCCCAAGCACAGATAACTGCAACTGGCCAGCAGCAACCGCAGGCC
CAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAGTTGATGGAACTGGGGATACATCA
TCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAGGAAGACAAAGAG
AAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGATGTG
AGTGATGAGGAAGGACAGGAACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGAT
AAGATACACAGAAGTAAAAACAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTT
AATGGAAGAGATTATATATTTTCCAAAGCCATTGGAGATGCAGAATGGTTG

Protein sequence predicted based on nucleotide sequence

MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFH
SEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIV
PDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQ
SVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTV
AAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKE
KDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNL
NGRDYIFSKAIGDAEWL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1302

Start on reference sequence 172

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pDONR221

Synonyms:

Sequencing Primer:

Forward: U19

Reverse: AttP2

Description:

Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;

Comments:

The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.

Size (bp):

4762

Parent Vector:

None

Properties:

Gateway, bacterial expression, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTAGTCGACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGCCAACTTAGTCGACCGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCAGCCTACTCGCTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAAGAAAAAGAGGTGCGAGCCTCTTTTTTGTGTGACAAAATAAAAACATCTACCTATTCATATACGCTAGTGTCATAGTCCTGAAAATCATCTGCATCAAGAACAATTTCACAACTCTTATACTTTTCTCTTACAAGTCGTTCGGCTTCATCTGGATTTTCAGCCTCTATACTTACTAAACGTGATAAAGTTTCTGTAATTTCTACTGTATCGACCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGGCGTTTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGACTACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

CDS

ccdB

CcdB, a bacterial toxin that poisons DNA gyrase

1197

1502

CDS

CmR

confers resistance to chloramphenicol

1847

2506

CDS

KanR

confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes

3156

3965

Terminator

rrnB T2

transcription terminator T2 from the E. coli rrnB gene

268

295

Terminator

rrnB T1

transcription terminator T1 from the E. coli rrnB gene

387

473

primer_bind

M13 forward

common sequencing primer, one of multiple similar variants

537

553

primer_bind

M13 reverse

common sequencing primer, one of multiple similar variants

3027

3043

promoter

cat_promoter-var002

promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase

2507

2609

promoter

T7 promoter

promoter for bacteriophage T7 RNA polymerase

3004

3022

protein binding site

attP1

recombination site for the Gateway BP reaction

570

801

protein binding site

attP2

recombination site for the Gateway BP reaction

2754

2985

rep_origin

ori

high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

4112

4700

Gene

Gene Symbol :

GTF2A1

Symbol Nomenclature :

GTF2A1

SYNONYM :

TF2A1; TFIIA; TFIIA-42; TFIIAL

Designation :

TFIIA alpha, p55|general transcription factor IIA subunit 1|glucose regulated protein, 58kD pseudogene|transcription initiation factor IIA subunit 1|transcription initiation factor TFIIA 42 kDa subunit

Full Nomenclature :

general transcription factor IIA, 1, 19/37kDa

GENEID :

2957

HGNC :

4646

MIM :

600520

Vega :

OTTHUMG00000171414

Target GenBank:

BC107156

Reference Sequence Alignment

  

HsCD00079572      ATGGCGAACTCGGCAAATACAAACACCGTGCCTAAATTATACAGATCTGTGATTGAAGAT
NM_015859.3       ATGGCGAACTCGGCAAATACAAACACCGTGCCTAAATTATACAGATCTGTGATTGAAGAT
                  ************************************************************

HsCD00079572      GTCATTAATGATGTGAGAGACATCTTTCTGGATGATGGAGTGGATGAACAAGTACTGATG
NM_015859.3       GTCATTAATGATGTGAGAGACATCTTTCTGGATGATGGAGTGGATGAACAAGTACTGATG
                  ************************************************************

HsCD00079572      GAACTAAAAACTTTATGGGAAAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCAT
NM_015859.3       GAACTAAAAACTTTATGGGAAAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCAT
                  ************************************************************

HsCD00079572      TCAGAAGAGCAGCAGCTTCTACTGCAAGTTCAACAGCAGCATCAACCCCAGCAGCAGCAG
NM_015859.3       TCAGAAGAGCAGCAGCTTCTACTGCAAGTTCAACAGCAGCATCAACCCCAGCAGCAGCAG
                  ************************************************************

HsCD00079572      CATCACCACCATCACCATCATCAGCAAGCTCAGCCTCAGCAGACAGTACCTCAGCAAGCG
NM_015859.3       CATCACCACCATCACCATCATCAGCAAGCTCAGCCTCAGCAGACAGTACCTCAGCAAGCG
                  ************************************************************

HsCD00079572      CAGACCCAGCAGGTTCTTATTCCTGCATCACAGCAAGCCACAGCACCACAAGTTATTGTT
NM_015859.3       CAGACCCAGCAGGTTCTTATTCCTGCATCACAGCAAGCCACAGCACCACAAGTTATTGTT
                  ************************************************************

HsCD00079572      CCAGATTCTAAGTTGATACAGCATATGAATGCATCAAACATGAGTGCTGCTGCTACAGCT
NM_015859.3       CCAGATTCTAAGTTGATACAGCATATGAATGCATCAAACATGAGTGCTGCTGCTACAGCT
                  ************************************************************

HsCD00079572      GCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATTAACAAATTCAGGC
NM_015859.3       GCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATTAACAAATTCAGGC
                  ************************************************************

HsCD00079572      CAGCTTCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAGCAG
NM_015859.3       CAGCTTCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAGCAG
                  ************************************************************

HsCD00079572      TCAGTGGTTCTACAACAACAGGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCT
NM_015859.3       TCAGTGGTTCTACAACAACAGGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCT
                  ************************************************************

HsCD00079572      GTTATACAGCAGGTGCTGGCTCCTCTTCCTGGAGGGATTTCACCACAGACAGGTGTCATC
NM_015859.3       GTTATACAGCAGGTGCTGGCTCCTCTTCCTGGAGGGATTTCACCACAGACAGGTGTCATC
                  ************************************************************

HsCD00079572      ATCCAGCCTCAGCAAATCTTATTTACAGGAAATAAGACTCAAGTTATACCTACGACAGTG
NM_015859.3       ATCCAGCCTCAGCAAATCTTATTTACAGGAAATAAGACTCAAGTTATACCTACGACAGTG
                  ************************************************************

HsCD00079572      GCAGCACCTACACCAGCCCAAGCACAGATAACTGCAACTGGCCAGCAGCAACCGCAGGCC
NM_015859.3       GCAGCACCTACACCAGCCCAAGCACAGATAACTGCAACTGGCCAGCAGCAACCGCAGGCC
                  ************************************************************

HsCD00079572      CAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAGTTGATGGAACTGGGGATACATCA
NM_015859.3       CAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAGTTGATGGAACTGGGGATACATCA
                  ************************************************************

HsCD00079572      TCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAGGAAGACAAAGAG
NM_015859.3       TCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAGGAAGACAAAGAG
                  ************************************************************

HsCD00079572      AAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGATGTG
NM_015859.3       AAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGATGTG
                  ************************************************************

HsCD00079572      AGTGATGAGGAAGGACAGGAACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGAT
NM_015859.3       AGTGATGAGGAAGGACAGGAACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGAT
                  ************************************************************

HsCD00079572      AAGATACACAGAAGTAAAAACAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTT
NM_015859.3       AAGATACACAGAAGTAAAAACAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTT
                  ************************************************************

HsCD00079572      AATGGAAGAGATTATATATTTTCCAAAGCCATTGGAGATGCAGAATGGTTG
NM_015859.3       AATGGAAGAGATTATATATTTTCCAAAGCCATTGGAGATGCAGAATGGTGA
                  ************************************************* .

Pathways

Panther :

General transcription regulation

Panther :

Transcription regulation by bZIP transcription factor

Reactome :

Disease

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

HIV Transcription Initiation

Reactome :

Late Phase of HIV Life Cycle

Reactome :

RNA Polymerase II HIV Promoter Escape

Reactome :

RNA Polymerase II Pre-transcription Events

Reactome :

RNA Polymerase II Promoter Escape

Reactome :

RNA Polymerase II Transcription

Reactome :

RNA Polymerase II Transcription Initiation

Reactome :

RNA Polymerase II Transcription Initiation And Promoter Clearance

Reactome :

RNA Polymerase II Transcription Pre-Initiation And Promoter Opening

Reactome :

Transcription

Reactome :

Transcription of the HIV genome

Protein

UniProt :

P52655

HPRD :

02748

Clone

Cloning Information :

257081

HIP Master Clone ID :

193129

Original Clone ID :

FLH257081.01L

Organism

Species Specific ID:

2957

Genome

Chromosome :

Map Location :

14q31.1

Ensembl :

ENSG00000165417

Reagents

Labome :

GTF2A1-antibody