DNASU Plasmid | ATOH1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ATOH1

Gene Name: atonal homolog 1 (Drosophila)

Map: pDONR cofig.pdf

Original Clone ID:

FLH257241.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pDONR221

Format:

FUSION

Source:

HIP

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HIP

HsCD00079687

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1065nts

Open reading frame: 1 to 1065

Reference Sequence Alignment

ATGTCCCGCCTGCTGCATGCAGAAGAGTGGGCTGAAGTGAAGGAGTTGGGAGACCACCAT
CGCCAGCCCCAGCCGCATCATCTCCCGCAACCGCCGCCGCCGCCGCAGCCACCTGCAACT
TTGCAGGCGAGAGAGCATCCCGTCTACCCGCCTGAGCTGTCCCTCCTGGACAGCACCGAC
CCACGCGCCTGGCTGGCTCCCACTTTGCAGGGCATCTGCACGGCACGCGCCGCCCAGTAT
TTGCTACATTCCCCGGAGCTGGGTGCCTCAGAGGCCGCTGCGCCCCGGGACGAGGTGGAC
GGCCGGGGGGAGCTGGTAAGGAGGAGCAGCGGCGGTGCCAGCAGCAGCAAGAGCCCCGGG
CCGGTGAAAGTGCGGGAACAGCTGTGCAAGCTGAAAGGCGGGGTGGTGGTAGACGAGCTG
GGCTGCAGCCGCCAACGGGCCCCTTCCAGCAAACAGGTGAATGGGGTGCAGAAGCAGAGA
CGGCTAGCAGCCAACGCCAGGGAGCGGCGCAGGATGCATGGGCTGAACCACGCCTTCGAC
CAGCTGCGCAATGTTATCCCGTCGTTCAACAACGACAAGAAGCTGTCCAAATATGAGACC
CTGCAGATGGCCCAAATCTACATCAACGCCTTGTCCGAGCTGCTACAAACGCCCAGCGGA
GGGGAACAGCCACCGCCGCCTCCAGCCTCCTGCAAAAGCGACCACCACCACCTTCGCACC
GCGGCCTCCTATGAAGGGGGCGCGGGCAACGCGACCGCAGCTGGGGCTCAGCAGGCTTCC
GGAGGGAGCCAGCGGCCGACCCCGCCCGGGAGTTGCCGGACTCGCTTCTCAGCCCCAGCT
TCTGCGGGAGGGTACTCGGTGCAGCTGGACGCTCTGCACTTCTCGACTTTCGAGGACAGC
GCCCTGACAGCGATGATGGCGCAAAAGAATTTGTCTCCTTCTCTCCCCGGGAGCATCTTG
CAGCCAGTGCAGGAGGAAAACAGCAAAACTTCGCCTCGGTCCCACAGAAGCGACGGGGAA
TTTTCCCCCCATTCCCATTACAGTGACTCGGATGAGGCAAGTTTG

Protein sequence predicted based on nucleotide sequence

MSRLLHAEEWAEVKELGDHHRQPQPHHLPQPPPPPQPPATLQAREHPVYPPELSLLDSTD
PRAWLAPTLQGICTARAAQYLLHSPELGASEAAAPRDEVDGRGELVRRSSGGASSSKSPG
PVKVREQLCKLKGGVVVDELGCSRQRAPSSKQVNGVQKQRRLAANARERRRMHGLNHAFD
QLRNVIPSFNNDKKLSKYETLQMAQIYINALSELLQTPSGGEQPPPPPASCKSDHHHLRT
AASYEGGAGNATAAGAQQASGGSQRPTPPGSCRTRFSAPASAGGYSVQLDALHFSTFEDS
ALTAMMAQKNLSPSLPGSILQPVQEENSKTSPRSHRSDGEFSPHSHYSDSDEASL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1065

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pDONR221

Synonyms:

Sequencing Primer:

Forward: U19

Reverse: AttP2

Description:

Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;

Comments:

The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.

Size (bp):

4762

Parent Vector:

None

Properties:

Gateway, bacterial expression, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTAGTCGACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGCCAACTTAGTCGACCGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCAGCCTACTCGCTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAAGAAAAAGAGGTGCGAGCCTCTTTTTTGTGTGACAAAATAAAAACATCTACCTATTCATATACGCTAGTGTCATAGTCCTGAAAATCATCTGCATCAAGAACAATTTCACAACTCTTATACTTTTCTCTTACAAGTCGTTCGGCTTCATCTGGATTTTCAGCCTCTATACTTACTAAACGTGATAAAGTTTCTGTAATTTCTACTGTATCGACCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGGCGTTTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGACTACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

CDS

ccdB

CcdB, a bacterial toxin that poisons DNA gyrase

1197

1502

CDS

CmR

confers resistance to chloramphenicol

1847

2506

CDS

KanR

confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes

3156

3965

Terminator

rrnB T2

transcription terminator T2 from the E. coli rrnB gene

268

295

Terminator

rrnB T1

transcription terminator T1 from the E. coli rrnB gene

387

473

primer_bind

M13 forward

common sequencing primer, one of multiple similar variants

537

553

primer_bind

M13 reverse

common sequencing primer, one of multiple similar variants

3027

3043

promoter

cat_promoter-var002

promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase

2507

2609

promoter

T7 promoter

promoter for bacteriophage T7 RNA polymerase

3004

3022

protein binding site

attP1

recombination site for the Gateway BP reaction

570

801

protein binding site

attP2

recombination site for the Gateway BP reaction

2754

2985

rep_origin

ori

high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

4112

4700

Gene

Gene Symbol :

ATOH1

Symbol Nomenclature :

ATOH1

SYNONYM :

ATH1; HATH1; MATH-1; bHLHa14

Designation :

class A basic helix-loop-helix protein 14|helix-loop-helix protein hATH-1|protein atonal homolog 1

Full Nomenclature :

atonal homolog 1 (Drosophila)

GENEID :

474

HGNC :

797

MIM :

601461

Vega :

OTTHUMG00000130972

Target GenBank:

BC069604

Reference Sequence Alignment

  

HsCD00079687      ATGTCCCGCCTGCTGCATGCAGAAGAGTGGGCTGAAGTGAAGGAGTTGGGAGACCACCAT
NM_005172.1       ATGTCCCGCCTGCTGCATGCAGAAGAGTGGGCTGAAGTGAAGGAGTTGGGAGACCACCAT
                  ************************************************************

HsCD00079687      CGCCAGCCCCAGCCGCATCATCTCCCGCAACCGCCGCCGCCGCCGCAGCCACCTGCAACT
NM_005172.1       CGCCAGCCCCAGCCGCATCATCTCCCGCAACCGCCGCCGCCGCCGCAGCCACCTGCAACT
                  ************************************************************

HsCD00079687      TTGCAGGCGAGAGAGCATCCCGTCTACCCGCCTGAGCTGTCCCTCCTGGACAGCACCGAC
NM_005172.1       TTGCAGGCGAGAGAGCATCCCGTCTACCCGCCTGAGCTGTCCCTCCTGGACAGCACCGAC
                  ************************************************************

HsCD00079687      CCACGCGCCTGGCTGGCTCCCACTTTGCAGGGCATCTGCACGGCACGCGCCGCCCAGTAT
NM_005172.1       CCACGCGCCTGGCTGGCTCCCACTTTGCAGGGCATCTGCACGGCACGCGCCGCCCAGTAT
                  ************************************************************

HsCD00079687      TTGCTACATTCCCCGGAGCTGGGTGCCTCAGAGGCCGCTGCGCCCCGGGACGAGGTGGAC
NM_005172.1       TTGCTACATTCCCCGGAGCTGGGTGCCTCAGAGGCCGCTGCGCCCCGGGACGAGGTGGAC
                  ************************************************************

HsCD00079687      GGCCGGGGGGAGCTGGTAAGGAGGAGCAGCGGCGGTGCCAGCAGCAGCAAGAGCCCCGGG
NM_005172.1       GGCCGGGGGGAGCTGGTAAGGAGGAGCAGCGGCGGTGCCAGCAGCAGCAAGAGCCCCGGG
                  ************************************************************

HsCD00079687      CCGGTGAAAGTGCGGGAACAGCTGTGCAAGCTGAAAGGCGGGGTGGTGGTAGACGAGCTG
NM_005172.1       CCGGTGAAAGTGCGGGAACAGCTGTGCAAGCTGAAAGGCGGGGTGGTGGTAGACGAGCTG
                  ************************************************************

HsCD00079687      GGCTGCAGCCGCCAACGGGCCCCTTCCAGCAAACAGGTGAATGGGGTGCAGAAGCAGAGA
NM_005172.1       GGCTGCAGCCGCCAACGGGCCCCTTCCAGCAAACAGGTGAATGGGGTGCAGAAGCAGAGA
                  ************************************************************

HsCD00079687      CGGCTAGCAGCCAACGCCAGGGAGCGGCGCAGGATGCATGGGCTGAACCACGCCTTCGAC
NM_005172.1       CGGCTAGCAGCCAACGCCAGGGAGCGGCGCAGGATGCATGGGCTGAACCACGCCTTCGAC
                  ************************************************************

HsCD00079687      CAGCTGCGCAATGTTATCCCGTCGTTCAACAACGACAAGAAGCTGTCCAAATATGAGACC
NM_005172.1       CAGCTGCGCAATGTTATCCCGTCGTTCAACAACGACAAGAAGCTGTCCAAATATGAGACC
                  ************************************************************

HsCD00079687      CTGCAGATGGCCCAAATCTACATCAACGCCTTGTCCGAGCTGCTACAAACGCCCAGCGGA
NM_005172.1       CTGCAGATGGCCCAAATCTACATCAACGCCTTGTCCGAGCTGCTACAAACGCCCAGCGGA
                  ************************************************************

HsCD00079687      GGGGAACAGCCACCGCCGCCTCCAGCCTCCTGCAAAAGCGACCACCACCACCTTCGCACC
NM_005172.1       GGGGAACAGCCACCGCCGCCTCCAGCCTCCTGCAAAAGCGACCACCACCACCTTCGCACC
                  ************************************************************

HsCD00079687      GCGGCCTCCTATGAAGGGGGCGCGGGCAACGCGACCGCAGCTGGGGCTCAGCAGGCTTCC
NM_005172.1       GCGGCCTCCTATGAAGGGGGCGCGGGCAACGCGACCGCAGCTGGGGCTCAGCAGGCTTCC
                  ************************************************************

HsCD00079687      GGAGGGAGCCAGCGGCCGACCCCGCCCGGGAGTTGCCGGACTCGCTTCTCAGCCCCAGCT
NM_005172.1       GGAGGGAGCCAGCGGCCGACCCCGCCCGGGAGTTGCCGGACTCGCTTCTCAGCCCCAGCT
                  ************************************************************

HsCD00079687      TCTGCGGGAGGGTACTCGGTGCAGCTGGACGCTCTGCACTTCTCGACTTTCGAGGACAGC
NM_005172.1       TCTGCGGGAGGGTACTCGGTGCAGCTGGACGCTCTGCACTTCTCGACTTTCGAGGACAGC
                  ************************************************************

HsCD00079687      GCCCTGACAGCGATGATGGCGCAAAAGAATTTGTCTCCTTCTCTCCCCGGGAGCATCTTG
NM_005172.1       GCCCTGACAGCGATGATGGCGCAAAAGAATTTGTCTCCTTCTCTCCCCGGGAGCATCTTG
                  ************************************************************

HsCD00079687      CAGCCAGTGCAGGAGGAAAACAGCAAAACTTCGCCTCGGTCCCACAGAAGCGACGGGGAA
NM_005172.1       CAGCCAGTGCAGGAGGAAAACAGCAAAACTTCGCCTCGGTCCCACAGAAGCGACGGGGAA
                  ************************************************************

HsCD00079687      TTTTCCCCCCATTCCCATTACAGTGACTCGGATGAGGCAAGTTTG
NM_005172.1       TTTTCCCCCCATTCCCATTACAGTGACTCGGATGAGGCAAGTTAG
                  *******************************************:*